RESEARCH ARTICLE
Improving intraoperative storage conditions for autologous bone grafts: An experimental investigation in mice
Qiang Sun,
Zhijun Li,
Bo Liu,
Xue Yuan,
Shu Guo,
Jill A. Helms,
Qiang Sun
Department of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA
Qiang Sun, Zhijun Li, and Bo Liu contributed equally.Search for more papers by this authorZhijun Li
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA
Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China
Qiang Sun, Zhijun Li, and Bo Liu contributed equally.Search for more papers by this authorBo Liu
Ankasa Regenerative Therapeutics, Inc., South San Francisco, CA
Qiang Sun, Zhijun Li, and Bo Liu contributed equally.Search for more papers by this authorXue Yuan
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA
Search for more papers by this authorShu Guo
Department of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China
Search for more papers by this authorCorresponding Author
Jill A. Helms
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA
Ankasa Regenerative Therapeutics, Inc., South San Francisco, CA
Correspondence
Jill A. Helms, Stanford University, 1651 Page Mill Road, Palo Alto, CA 94304.
Email: [email protected]
Search for more papers by this authorQiang Sun,
Zhijun Li,
Bo Liu,
Xue Yuan,
Shu Guo,
Jill A. Helms,
Qiang Sun
Department of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA
Qiang Sun, Zhijun Li, and Bo Liu contributed equally.Search for more papers by this authorZhijun Li
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA
Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China
Qiang Sun, Zhijun Li, and Bo Liu contributed equally.Search for more papers by this authorBo Liu
Ankasa Regenerative Therapeutics, Inc., South San Francisco, CA
Qiang Sun, Zhijun Li, and Bo Liu contributed equally.Search for more papers by this authorXue Yuan
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA
Search for more papers by this authorShu Guo
Department of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China
Search for more papers by this authorCorresponding Author
Jill A. Helms
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA
Ankasa Regenerative Therapeutics, Inc., South San Francisco, CA
Correspondence
Jill A. Helms, Stanford University, 1651 Page Mill Road, Palo Alto, CA 94304.
Email: [email protected]
Search for more papers by this authorAbstract
Autologous bone grafts constitute the second most transplanted tissue in medicine today. The viability, and consequently the osteogenic capacity, of an autograft is directly impacted by the interval between harvest and transplantation, but how the temperature and the solution in which the graft is held intraoperatively affect viability is not clear. Using a syngeneic mouse model and in vivo bone-forming assays, these variables were tested for their effects on programmed cell death, osteoprogenitor cell proliferation, and the ability of the autograft to ultimately produce new bone in an ectopic site. Based on these results, the intraoperative treatment with a WNT protein therapeutic was tested for its effects on the viability and osteogenic capacity of an autograft. Viability, programmed cell death, mitotic activity, osteogenic protein expression, and bone-forming capacity were assessed. Experimental results demonstrated that the osteogenic capacity of an autograft is significantly improved by intraoperative storage in L-WNT3A at physiological temperature.
CONFLICT OF INTEREST
B.L and J.A.H. are employees of Ankasa Regenerative Therapeutics, a biopharmaceutic company developing L-WNT3A for commercial use.
Supporting Information
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Data S1. Supporting information |
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