RESEARCH ARTICLE
Screening and optimization of potential injection vehicles for storage of retinal pigment epithelial stem cell before transplantation
Yangzi Tian,
Richard Davis,
Michael R. Zonca Jr,
Jeffrey H. Stern,
Sally Temple,
Yubing Xie,
Yangzi Tian
Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York
Search for more papers by this authorRichard Davis
Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York
Search for more papers by this authorMichael R. Zonca Jr
Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York
Search for more papers by this authorJeffrey H. Stern
Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York
Search for more papers by this authorSally Temple
Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York
Search for more papers by this authorCorresponding Author
Yubing Xie
Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York
Correspondence
Yubing Xie, Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203.
Email: [email protected]
Search for more papers by this authorYangzi Tian,
Richard Davis,
Michael R. Zonca Jr,
Jeffrey H. Stern,
Sally Temple,
Yubing Xie,
Yangzi Tian
Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York
Search for more papers by this authorRichard Davis
Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York
Search for more papers by this authorMichael R. Zonca Jr
Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York
Search for more papers by this authorJeffrey H. Stern
Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York
Search for more papers by this authorSally Temple
Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York
Search for more papers by this authorCorresponding Author
Yubing Xie
Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York
Correspondence
Yubing Xie, Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, 257 Fuller Road, Albany, NY 12203.
Email: [email protected]
Search for more papers by this authorAbstract
Retinal pigment epithelial (RPE) cells are highly specialized neural cells that have several functions essential for vision. Progressive deterioration of RPE cells in elderly individuals can result in visual impairment and ultimately the blinding disease age-related macular degeneration. Subretinal transplantation of stem cell-derived RPE cell suspensions is being explored as a strategy to recover the damaged retina and improve vision. This approach may be improved by developing a vehicle that increases postinjection cell viability and distribution and integration of RPE cells. In this study, Food and Drug Administration-approved natural polymers, including alginate, methylcellulose, and hyaluronic acid (HA), were examined for performance as cell vehicles for adult human RPE stem cells (RPESCs). We compared the effect of RPESC storage as a cell suspension in these delivery vehicles for 1–96 hr at different temperatures on subsequent cell performance in a cell culture model. RPESC survival, attachment, distribution, proliferation, and differentiation into RPE cells were monitored by microscopy over the course of 8 weeks. Our in vitro results demonstrate that RPESC suspension in a 0.2% HA solution promotes better initial cell distribution, proliferation, cobblestone formation, and expression of RPE cell markers (microphthalmia-associated transcription factor and orthodenticle homeobox 2) after 96 hr of storage. These data suggest that HA addition to the vehicle can significantly enhance RPESC performance in vitro and is a promising strategy to pursue an improved delivery vehicle supporting in vivo RPE cell transplantation.
CONFLICT OF INTEREST
The authors have no conflict of interest to declare.
Supporting Information
| Filename | Description |
|---|---|
| term2770-sup-0001-sf1-sf2.docxWord 2007 document , 902 KB |
Figure S1 Viability of RPESCs after storage in HA from Sigma-Aldrich at different temperatures, (A) Room temperature. (B) 12°C. (C) 4°C. * p < 0.05. ** p < 0.01. *** p < 0.001. **** p < 0.0001. Figure S2 Viability of RPESCs after storage in Provisc HA at different temperatures, (A) Room temperature. (B) 12°C. (C) 4°C. * p < 0.05. * p < 0.05. ** p < 0.01. *** p < 0.001. **** p < 0.0001. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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