Bioglass enhanced wound healing ability of urine-derived stem cells through promoting paracrine effects between stem cells and recipient cells
Correction(s) for this article
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Corrigendum
- Volume 13Issue 11Journal of Tissue Engineering and Regenerative Medicine
- pages: 2126-2126
- First Published online: November 20, 2019
Yunlong Zhang
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorXin Niu
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorXin Dong
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorYang Wang
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorCorresponding Author
Haiyan Li
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Correspondence
Haiyan Li, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.
Email: [email protected]
Search for more papers by this authorYunlong Zhang
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorXin Niu
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorXin Dong
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorYang Wang
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorCorresponding Author
Haiyan Li
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
Correspondence
Haiyan Li, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.
Email: [email protected]
Search for more papers by this authorAbstract
In cell therapy, tissue regeneration ability of stem cells relies on the paracrine effects between stem cells and recipient cells. Our recent studies demonstrated that, in tissue engineering, bioactive silicates could stimulate paracrine effects between stem cells and recipient cells, which enhanced tissue generation. Therefore, we proposed that, in cell therapy, it may be an effective method to improve tissue regeneration ability of stem cells through activating the paracrine effects between stem cells and recipient cells with bioactive silicates. As urine-derived stem cells (USCs) have been injected for wound healing and bioglass (BG) have shown bioactivity for various types of stem cells, in this study, we activated USCs with effective BG ionic products. Then the conditioned medium of BG-activated USCs were used to culture endothelial cells and fibroblasts as well as co-cultures of endothelial cells and fibroblasts. Results showed that growth factor expression in BG-activated USCs was upregulated. In addition, paracrine effects between USCs and recipient cells in wound healing were stimulated, which resulted in enhanced capillary-like network formation of endothelial cells and matrix protein production as well as myofibroblast differentiation of fibroblasts. Finally, the BG-activated USCs were applied on full-thickness excisional wounds. Results confirmed that BG-activated USCs had better wound healing ability through improving angiogenesis and collagen deposition in wound sites as compared with USCs without any treatment. Taken together, BG can be used to promote wound healing ability of USCs by enhancing paracrine effects between USCs and recipient cells.
Supporting Information
| Filename | Description |
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| term2587-sup-0001-DataS1.docxWord 2007 document , 229 KB |
The Supporting Information demonstrates the effects of USC CM-BG on downstreaming angiogenic factors in a direct HDF-HUVEC co-culture and the gene sequence of primers used in this study. Data S1. Figure S1 Effects of USC CM-BG on downstreaming angiogenic factors in a direct HDF-HUVEC co-culture. (A) Gene expression of VE-cad was upregulated by USC CM-BG as compared to USC CM. (B) Gene expression of eNOS was upregulated by USC CM-BG as compared to USC CM. (C) Immunofluorescence staining further confirmed the upregulation of VE-cad by USC CM-BG. In addition, NO expression in the co-cultures was also enhanced by USC CM-BG. Table S1 Gene sequence of primers used in this study. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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