Effect of actinomycin D on early axial development in chick embryos^†

Rochelle M. Wolkowski,

Rochelle M. Wolkowski

Department of Animal Genetics, University of Connecticut, Storrs, Connecticut 06268

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Rochelle M. Wolkowski,

Rochelle M. Wolkowski

Department of Animal Genetics, University of Connecticut, Storrs, Connecticut 06268

Search for more papers by this author

First published: November 1970

https://doi.org/10.1002/tera.1420030416

Citations: 2

^†

Scientific contribution no. 413 of the Agricultural Experiment Station, University of Connecticut. Contribution no. 185 of the Institute of Cellular Biology. Supported in part by Fellowship GM-33,242 from the National Institute of General Medical Sciences, Public Health Service and by grant GB 4714 from the National Science Foundation (administered by L. J. Pierro).

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Abstract

A study was made of the uptake of actinomycin D, ³H-thymidine, and ³H-uridine in the axial regions of 11- to 13-somite chick embryos cultured in vitro. Actinomycin D was taken up into all regions of embryos, with significantly more actinomycin D per cell in the axial region, including the node and unsegmented axial mesoderm (the lower axis). Demonstrable effects on RNA synthesis were restricted to tissue in the lower axial region. However, inhibition of DNA synthesis was seen in tissues of the upper axis already segmented, as well as in the lower axis. The differential response of the embryonic axis to actinomycin D is considered to be based on a greater demand for cells in the unsegmented and nodal region. Actinomycin D inhibited RNA synthesis, thereby inhibited DNA synthesis and cell division, and reduced availability of cells for growth and development. The paucity of cells in the node and unsegmented axial mesoderm restricted the formation of new somites and the laying down of new nerve cord and notochordal tissue. The upper axial region was not permanently affected.

Literature Cited

Baserga, R., R. D. Estensen, R. O. Peterson and J. P. Layde 1965a Inhibition of DNA synthesis in Ehrlich ascites cells by actinomycin D. 1. Delayed inhibition by low doses. Proc. Nat. Acad. Sci. U. S., 54: 745–751.
10.1073/pnas.54.3.745
CAS PubMed Web of Science® Google Scholar
Baserga, R., R. D. Estensen and R. O. Peterson 1965b Inhibition of DNA synthesis in Ehrlich ascites cells by actinomycin D. 2. The presynthetic block in the cell cycle. Proc. Nat. Acad. Sci. U. S., 54: 1141–1148.
10.1073/pnas.54.4.1141
CAS PubMed Web of Science® Google Scholar
Britt, L. G., and H. Herrmann 1959 Protein accumulation in early chick embryos grown under different conditions of explantation. J. Embryol. Exp. Morph., 7: 66–72.
CAS PubMed Web of Science® Google Scholar
Burton, K. 1956 A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J., 62: 315–323.
10.1042/bj0620315
CAS PubMed Web of Science® Google Scholar
Dingman, C. W., and M. B. Sporn 1964 Studies on chromatin. I. Isolation and characterization of nuclear complexes of deoxyribonucleic acid, ribonucleic acid, and protein from embryonic and adult tissues of the chicken. J. Biol. Chem., 239: 3483–3492.
CAS PubMed Web of Science® Google Scholar
Dingman, C. W., 1965 Actinomycin D and hydrocortisone: intracellular binding in rat liver. Science, 149: 1251–1254.
10.1126/science.149.3689.1251
PubMed Web of Science® Google Scholar
Giles, K. W., and A. Myers 1965 An improved diphenylamine method for the estimation of deoxyribonucleic acid. Nature, 205: 93.
10.1038/206093a0
PubMed Google Scholar
Girard, M., S. Penman and J. E. Darnell 1969 The effect of actinomycin on ribosome formation in HeLa cells. Proc. Nat. Acad. Sci. U. S., 51: 205–211.
10.1073/pnas.51.2.205
Web of Science® Google Scholar
Goldberg, I. H., and E. Reich 1964 Actinomycin inhibition of RNA synthesis directed by DNA. Fed. Proc., 23: 958–964.
CAS PubMed Web of Science® Google Scholar
Hamilton, H. L. 1952 Lillie's Development of the Chick. 3rd Ed. Holt, New York.
Google Scholar
Harel, L., J. Harel., A. Boer, J. Imbenotte and N. Carpeni 1964 Persistance d'une synthese de l'RNA dans le foie de rat traité par l'actinomycine. Biochim. Biophys. Acta, 87: 212–218.
CAS PubMed Web of Science® Google Scholar
Harris, H. 1964 Breakdown of nuclear ribonucleic acid in the presence of actinomycin D. Nature, 202: 1301–1303.
10.1038/2021301a0
CAS PubMed Web of Science® Google Scholar
Heilporn-Pohl, V. 1964 Effets de l'actinomycine D sur la morphogenèse et le métabolisme des acides nucleiques chez l'embryon de poulet. J. Embryol. Exp. Morph., 12: 439–446.
CAS PubMed Web of Science® Google Scholar
Honig, G. R., and M. Rabinovitz 1965 Actinomycin D: inhibition of protein synthesis unrelated to effect on template RNA synthesis. Science, 149: 1504–1506.
10.1126/science.149.3691.1504
CAS PubMed Web of Science® Google Scholar
Hutchison, W. C., and H. N. Munro 1961 The determination of nucleic acids in biological materials. A review. Analyst, 86: 768–813.
10.1039/an9618600768
CAS PubMed Web of Science® Google Scholar
Kay, J. E., and H. L. Cooper 1969 Differential inhibition of 28S and 18S ribosomal RNA synthesis by actinomycin. Biochem. Biophys. Res. Comm., 35: 526–530.
10.1016/0006-291X(69)90378-7
CAS PubMed Web of Science® Google Scholar
Klein, N. W., E. McConnell and B. J. Buckingham 1962a Growth of explanted chick embryos on a chemically defined medium and effects of specific amino acid deficiencies. Dev. Biol., 5: 296–308.
10.1016/0012-1606(62)90015-5
CAS PubMed Web of Science® Google Scholar
Klein, N. W., E. McConnell and D. J. Riquier 1962b Effects of high oxygen levels on the growth and survival of explanted chick embryos. Am. Zool., 2: 420 (abst.).
Web of Science® Google Scholar
Klein, N. W., 1964 Enhanced growth and survival of explanted chick embryos cultured under high levels of oxygen. Dev. Biol., 10: 17–44.
10.1016/0012-1606(64)90003-X
CAS PubMed Web of Science® Google Scholar
Klein, N. W., and L. J. Pierro 1963 Actinomycin D: specific inhibitory effects on the explanted chick embryo. Science, 142: 967–969.
10.1126/science.142.3594.967
CAS PubMed Web of Science® Google Scholar
Levinthal, C., D. P. Fan, A. Higa and R. A. Zimmerman 1963 The decay and protection of messenger RNA in bacteria. C. S. H. Symp. Quant. Biol., 28: 183–190.
10.1101/SQB.1963.028.01.030
CAS Web of Science® Google Scholar
Liao, S., R. W. Barton and A. H. Lin 1966 Differential synthesis of ribonucleic acid in prostatic nuclei: evidence for selective gene transcription induced by androgens. Proc. Nat. Acad. Sci. U. S., 55: 1593–1600.
10.1073/pnas.55.6.1593
CAS PubMed Web of Science® Google Scholar
Liao, S., and A. H. Lin 1967 Prostatic nuclear chromat In: an effect of testosterone on the synthesis of ribonucleic acid rich in cytidylyl-(3′,5′)guanosine. Proc. Nat. Acad. Sci. U. S., 57: 379–386.
10.1073/pnas.57.2.379
CAS PubMed Web of Science® Google Scholar
Love, R., G. P. Studzinski and K. A. O. Ellem 1965 Use of antimetabolites in the study of normal and abnormal cell structure and function. Fed. Proc., 24: 1206–1216.
CAS PubMed Google Scholar
Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall 1951 Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265–275.
10.1016/S0021-9258(19)52451-6
CAS PubMed Web of Science® Google Scholar
Oyama, V. I., and H. Eagle 1956 Measurements of cell growth in tissue culture with phenol reagent (Folin-Ciocalteau). Proc. Soc. Exp. Biol. Med., 91: 305–307.
10.3181/00379727-91-22245
CAS PubMed Web of Science® Google Scholar
Perry, R. P. 1963 Selective effects of actinomycin D on the intracellular distribution of RNA synthesis in tissue culture cells. Exp. Cell Res., 29: 400–406.
10.1016/S0014-4827(63)80003-8
CAS Web of Science® Google Scholar
Pierro, L. J. 1961a Teratogenic action of actinomycin D in the embryonic chick. J. Exp. Zool., 147: 203–210.
10.1002/jez.1401470302
CAS PubMed Web of Science® Google Scholar
Pierro, L. J. 1961b Teratogenic action of actinomycin D in the embryonic chick. 2. Early development. J. Exp. Zool., 148: 241–249.
10.1002/jez.1401480306
CAS PubMed Web of Science® Google Scholar
Pierro, L. J., and J. E. Coghill 1968 Interaction in teratogenesis: actinomycin D, puromycin and deoxyguanosine. Teratology, 1: 21–32.
10.1002/tera.1420010105
CAS PubMed Web of Science® Google Scholar
Piper, J. A., and N. W. Klein 1969 The differential sensitivity of cultured chick mesodermal cells to actinomycin D. J. Cell Biol., 40: 209–215.
10.1083/jcb.40.1.209
CAS PubMed Web of Science® Google Scholar
Scherrer, K., H. Latham and J. E. Darnell 1963 Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells. Proc. Nat. Acad. Sci. U. S., 49: 240–248.
10.1073/pnas.49.2.240
CAS PubMed Web of Science® Google Scholar
Schmidt, G., and J. J. Thannhauser 1945 A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues. J. Biol. Chem., 161: 83–89.
10.1016/S0021-9258(17)41524-9
CAS PubMed Web of Science® Google Scholar
Schneider, W. C. 1957 Determination of nucleic acids in tissues by pentose analysis. In: Methods in Enzymology. Vol. 3. S. P. Colowick and N. O. Kaplan, eds. Academic Press, New York, pp. 315–323.
Google Scholar
Spratt, N. T.Jr., 1947 Development in vitro of the early chick blastoderm explanted on yolk and albumen extract saline-agar substrata. J. Exp. Zool., 106: 345–366.
10.1002/jez.1401060308
PubMed Web of Science® Google Scholar
Weisner, R., E. Acs, E. Reich and A. Shatig 1965 Degradation of ribonucleic acid in mouse fibroblasts treated with actinomycin. J. Cell Biol., 27: 47–52.
10.1083/jcb.27.1.47
PubMed Web of Science® Google Scholar

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Volume3, Issue4

November 1970

Pages 389-397

Effect of actinomycin D on early axial development in chick embryos^†

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Effect of actinomycin D on early axial development in chick embryos†

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Effect of actinomycin D on early axial development in chick embryos^†