An Integrated, Portable, and Automatic Digital Detection System for Hepatitis B Virus Using Hybrid Magnetic System
Xiaoying Han
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058 China
Search for more papers by this authorCorresponding Author
Juxin Yin
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
School of Information and Electrical Engineering, Hangzhou City University, Hangzhou, Zhejiang Province, 310015 China
Academy of Edge Intelligence, Hangzhou City University, Hangzhou, Zhejiang Province, 310015 China
E-mail: [email protected][email protected]
Search for more papers by this authorYu Wang
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
Search for more papers by this authorJianjian Zhuang
Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, 310006 China
Search for more papers by this authorKai Hu
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
Search for more papers by this authorYehong Gui
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058 China
Search for more papers by this authorHaohua Mei
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058 China
Search for more papers by this authorJizhi Tong
School of Information and Electrical Engineering, Hangzhou City University, Hangzhou, Zhejiang Province, 310015 China
Search for more papers by this authorCorresponding Author
Ying Mu
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
E-mail: [email protected][email protected]
Search for more papers by this authorXiaoying Han
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058 China
Search for more papers by this authorCorresponding Author
Juxin Yin
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
School of Information and Electrical Engineering, Hangzhou City University, Hangzhou, Zhejiang Province, 310015 China
Academy of Edge Intelligence, Hangzhou City University, Hangzhou, Zhejiang Province, 310015 China
E-mail: [email protected][email protected]
Search for more papers by this authorYu Wang
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
Search for more papers by this authorJianjian Zhuang
Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, 310006 China
Search for more papers by this authorKai Hu
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
Search for more papers by this authorYehong Gui
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058 China
Search for more papers by this authorHaohua Mei
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
School of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058 China
Search for more papers by this authorJizhi Tong
School of Information and Electrical Engineering, Hangzhou City University, Hangzhou, Zhejiang Province, 310015 China
Search for more papers by this authorCorresponding Author
Ying Mu
College of Control Science and Engineering, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027 China
E-mail: [email protected][email protected]
Search for more papers by this authorAbstract
The rapid, precise, and automated diagnosis of infectious diseases is crucial for effective disease management and control. Herein, the integrated portable and automatic digital detection system (IPADS), a novel diagnostic platform for nucleic acid detection is introduced. The device employs the hybrid magnetic system (HMS), which uses an electromagnet and a movable permanent magnet to modulate the magnetic field and control bead movement, increasing nucleic acid extraction efficiency to over 80%, while simplifying the traditional labor-intensive process and enabling quick, low-risk sample processing. Additionally, a disposable cartridge is designed for integrated HMS based preprocessing, with detection performed using digital RPA-Cas12a, enabling rapid, enclosed, and automation-friendly detection across a dynamic range spanning five orders of magnitude, with a sensitivity as low as 100 copies mL−1 in serum samples. An automated platform further optimizes workflow. As a proof of concept, IPADS is applied to detect hepatitis B virus (HBV) DNA in 20 clinical serum samples, demonstrating high concordance with gold-standard quantitative PCR (qPCR) methods. These results validate the potential of IPADS as a reliable point-of-care testing solution.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| smtd202401971-sup-0001-SuppMat.docx1.7 MB | Supporting Information |
| smtd202401971-sup-0002-VideoS1.mp427.9 MB | Supplemental Video 1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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