Anchoring Group Mediated Radiolabeling for Achieving Robust Nanoimaging Probes
Lei Chen
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorJianxian Ge
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorBaoxing Huang
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorDandan Zhou
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorGang Huang
Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai, 201318 China
Search for more papers by this authorCorresponding Author
Jianfeng Zeng
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
E-mail: [email protected]
Search for more papers by this authorMingyuan Gao
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorLei Chen
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorJianxian Ge
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorBaoxing Huang
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorDandan Zhou
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorGang Huang
Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai, 201318 China
Search for more papers by this authorCorresponding Author
Jianfeng Zeng
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
E-mail: [email protected]
Search for more papers by this authorMingyuan Gao
Center for Molecular Imaging and Nuclear Medicine, State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123 China
Search for more papers by this authorAbstract
Radiolabeling counts for much in the functionalization of inorganic nanoparticles (NPs) because it endows NPs with high-sensitive imaging capacities apart from providing accurate pharmacokinetic information on the labeled particles, which makes the development of relevant radiolabeling chemistry highly desirable. Herein, a novel Ligand Anchoring Group MEdiated RAdioLabeling (LAGMERAL) method is reported, in which a polyethylene glycol (PEG) ligand with a diphosphonate (DP) terminal group plays a key role. It offers possibilities to radiolabel NPs through the spare coordination sites of the DP anchoring group. Through X-ray absorption spectroscopy studies, the coordination states of the foreign metal ions on the particle surface are investigated. In addition, radioactive Fe3O4 NPs are prepared by colabeling the particles with 125I at the outskirt of the particles through a phenolic hydroxyl moiety of the PEG ligand, and 99mTc at the root of the ligand, respectively. In this way, the stabilities of these types of radiolabeling are compared both in vitro and in vivo to show the advantages of the LAGMERAL method. The outstanding stability of probe and simplicity of the labeling process make the current approach universal for creating advanced NPs with different combinations of functionalities of the inorganic NPs and radioactive properties of the metal radioisotopes.
Conflict of interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
Research data are not shared.
Supporting Information
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References
- 1J. Zeng, L. Jing, Y. Hou, M. Jiao, R. Qiao, Q. Jia, C. Liu, F. Fang, H. Lei, M. Gao, Adv. Mater. 2014, 26, 2694.
- 2F. Li, Z. Liang, J. Liu, J. Sun, X. Hu, M. Zhao, J. Liu, R. Bai, D. Kim, X. Sun, T. Hyeon, D. Ling, Nano Lett. 2019, 19, 4213.
- 3H. Wei, O. Bruns, M. Kaul, E. Hansen, M. Barch, A. Wisniowska, O. Chen, Y. Chen, N. Li, S. Okada, J. Cordero, M. Heine, C. Farrar, D. Montana, G. Adam, H. Ittrich, A. Jasanoff, P. Nielsen, M. Bawendi, Proc. Natl. Acad. Sci. USA 2017, 114, 2325.
- 4Y. Lu, Y. Xu, G. Zhang, D. Ling, M. Wang, Y. Zhou, Y. Wu, T. Wu, M. Hackett, B. Kim, H. Chang, J. Kim, X. Hu, L. Dong, N. Lee, F. Li, J. He, L. Zhang, H. Wen, S. C. B. Yang, T. Hyeon, D. Zou, Nat. Biomed. Eng. 2017, 1, 637.
- 5C. Liang, X. Zhang, X. Z. Cheng, M. Yang, W. Huang, X. Dong, View 2020, 1, 20200046.
- 6R. Zhang, B. Le, W. Xu, K. Guo, X. Sun, H. Su, L. Huang, J. Huang, T. Shen, T. Liao, Y. Liang, J. X. J. Zhang, H. Dai, K. Qian, Small Methods 2019, 3, 1800474.
- 7Z. Ma, F. Wang, Y. Zhong, F. Salazar, J. Li, M. Zhang, F. Ren, A. Wu, H. Dai, Angew. Chem., Int. Ed. 2020, 59, 20552.
- 8L. Ye, K. Yong, L. Liu, I. Roy, R. Hu, J. Zhu, H. Cai, W. Law, J. Liu, K. Wang, J. Liu, Y. Liu, Y. Hu, X. Zhang, M. Swihart, P. Prasad, Nat. Nanotechnol. 2012, 7, 453.
- 9D. Naczynski, M. Tan, M. Zevon, B. Wall, J. Kohl, A. Kulesa, S. Chen, C. Roth, R. Riman, P. Moghe, Nat. Commun. 2013, 4, 2199.
- 10Y. Hou, R. Qiao, F. Fang, X. Wang, C. Dong, K. Liu, C. Liu, Z. Liu, H. Lei, F. Wang, M. Gao, ACS Nano 2013, 7, 330.
- 11J. Yang, R. Wang, L. Huang, M. Zhang, J. Niu, C. Bao, N. Shen, M. Dai, Q. Guo, Q. Wang, Q. Wang, Q. Fu, K. Qian, Angew. Chem., Int. Ed. 2020, 59, 1703.
- 12J. Cao, X. Shi, D. D. Gurav, L. Huang, H. Su, K. Li, J. Niu, M. Zhang, Q. Wang, M. Jiang, K. Qian, Adv. Mater. 2020, 32, 2000906.
- 13S. Zhang, C. Sun, J. Zeng, Q. Sun, G. Wang, Y. Wang, Y. Wu, S. Dou, M. Gao, Z. Li, Adv. Mater. 2016, 28, 8927.
- 14D. Kim, J. Kim, Y. Park, N. Lee, T. Hyeon, ACS Cent. Sci. 2018, 4, 324.
- 15B. R. Smith, S. S. Gambhir, Chem. Rev. 2017, 117, 901.
- 16L. Jing, C. Yang, P. Zhang, J. Zeng, Z. Li, M. Gao, View 2020, 1, e19.
10.1002/viw2.19 Google Scholar
- 17P. Bouziotis, D. Psimadas, T. Tsotakos, D. Stamopoulos, C. Tsoukalas, Curr. Top. Med. Chem. 2012, 12, 2694.
- 18J. Lamb, J. P. Holland, J. Nucl. Med. 2018, 59, 382.
- 19J. Ge, Q. Zhang, J. Zeng, Z. Gu, M. Gao, Biomaterials 2020, 228, 119553.
- 20C. T. Yang, K. K. Ghosh, P. Padmanabhan, O. Langer, J. Liu, D. N. C. Eng, C. Halldin, B. Gulyas, Theranostics 2018, 8, 6210.
- 21C. Perez-Medina, A. J. P. Teunissen, E. Kluza, W. J. M. Mulder, R. van der Meel, Adv. Drug Delivery Rev. 2020, 154–155, 123.
- 22R. Weissleder, D. D. Stark, B. L. Engelstad, B. R. Bacon, C. C. Compton, D. L. White, P. Jacobs, J. Lewis, Am. J. Roentgenol. 1989, 152, 167.
- 23S. Majumdar, S. S. Zoghbi, J. C. Gore, Invest. Radiol. 1990, 25, 771.
- 24J. Pellico, J. Ruiz-Cabello, M. Saiz-Alia, G. Del Rosario, S. Caja, M. Montoya, L. Fernandez de Manuel, M. P. Morales, L. Gutierrez, B. Galiana, J. A. Enriquez, F. Herranz, Contrast Media Mol. Imaging 2016, 11, 203.
- 25J. Llop, P. Jiang, M. Marradi, V. Gomez-Vallejo, M. Echeverria, S. Yu, M. Puigivila, Z. Baz, B. Szczupak, C. Perez-Campana, Z. Mao, C. Gao, S. E. Moya, J. Mater. Chem. B 2015, 3, 6293.
- 26R. M. Wong, D. A. Gilbert, K. Liu, A. Y. Louie, ACS Nano 2012, 6, 3461.
- 27J. Zeng, B. Jia, R. Qiao, C. Wang, L. Jing, F. Wang, M. Gao, Chem. Commun. 2014, 50, 2170.
- 28B. Freund, B. U. I. Tromsdorf, O. T. Bruns, M. Heine, A. Giemsa, A. Bartelt, S. C. Salmen, N. Raabe, J. Heeren, H. Ittrich, R. Reimer, H. Hohenberg, U. Schumacher, H. Weller, P. Nielsen, ACS Nano 2012, 6, 7318.
- 29R. Chakravarty, H. F. Valdovinos, F. Chen, C. M. Lewis, P. A. Ellison, H. Luo, M. E. Meyerand, R. J. Nickles, W. Cai, Adv. Mater. 2014, 26, 5119.
- 30E. Boros, A. M. Bowen, L. Josephson, N. Vasdev, J. P. Holland, Chem. Sci. 2015, 6, 225.
- 31H. Yuan, M. Q. Wilks, M. D. Normandin, G. El Fakhri, C. Kaittanis, L. Josephson, Nat. Protoc. 2018, 13, 392.
- 32M. D. Normandin, H. Yuan, M. Q. Wilks, H. H. Chen, J. M. Kinsella, H. Cho, N. J. Guehl, N. Absi-Halabi, S. M. Hosseini, G. El Fakhri, D. E. Sosnovik, L. Josephson, Angew. Chem., Int. Ed. 2015, 54, 13002.
- 33C. Glaus, R. Rossin, M. J. Welch, G. Bao, Bioconjugate Chem. 2010, 21, 715.
- 34H. Y. Lee, Z. Li, K. Chen, A. R. Hsu, C. Xu, J. Xie, S. Sun, X. Chen, J. Nucl. Med. 2008, 49, 1371.
- 35H. Wang, R. Kumar, D. Nagesha, R. I. Duclos, S. Sridhar, S. J. Gatley, Nucl. Med. Biol. 2015, 42, 65.
- 36S. Shanehsazzadeh, C. Grüttner, H. Yousefnia, A. Lahooti, A. Gholami, S. Nosrati, S. Zolghadri, S. H. M. Anijdan, A. Lotfabadi, B. Shiri Varnamkhasti, F. J. Daha, A. R. Jalilian, Radiochim. Acta 2016, 104, 337.
- 37B. P. Burke, N. Baghdadi, G. S. Clemente, N. Camus, A. Guillou, A. E. Kownacka, J. Domarkas, Z. Halime, R. Tripier, S. J. Archibald, Faraday Discuss. 2014, 175, 59.
- 38B.-B. Cho, J. H. Park, S. J. Jung, J. Lee, J. H. Lee, M. G. Hur, C. Justin Raj, K.-H. Yu, J. Radioanal. Nucl. Chem. 2015, 305, 169.
- 39S. Liu, B. Jia, R. Qiao, Z. Yang, Z. Yu, Z. Liu, K. Liu, J. Shi, H. Ouyang, F. Wang, M. Gao, Mol. Pharmaceutics 2009, 6, 1074.
- 40Y. Zhao, M. Liang, X. Li, K. Fan, J. Xiao, Y. Li, H. Shi, F. Wang, H. S. Choi, D. Cheng, X. Yan, ACS Nano 2016, 10, 4184.
- 41J. Wang, H. Zhao, Z. Zhou, P. Zhou, Y. Yan, M. Wang, H. Yang, Y. Zhang, S. Yang, ACS Appl. Mater. Interfaces 2016, 8, 19872.
- 42W. G. Kreyling, A. M. Abdelmonem, Z. Ali, F. Alves, M. Geiser, N. Haberl, R. Hartmann, S. Hirn, D. J. de Aberasturi, K. Kantner, G. Khadem-Saba, J. M. Montenegro, J. Rejman, T. Rojo, I. R. de Larramendi, R. Ufartes, A. Wenk, W. J. Parak, Nat. Nanotechnol. 2015, 10, 619.
- 43Z. Gao, Y. Hou, J. Zeng, L. Chen, C. Liu, W. Yang, M. Gao, Adv. Mater. 2017, 29, 1701095.
- 44C. Liu, Z. Gao, J. Zeng, Y. Hou, F. Fang, Y. Li, R. Qiao, L. Shen, H. Lei, W. Yang, M. Gao, ACS Nano 2013, 7, 7227.
- 45M. Jiao, L. Jing, C. Liu, Y. Hou, J. Huang, X. Wei, M. Gao, Chem. Commun. 2016, 52, 5872.
- 46R. Torres Martin de Rosales, R. Tavare, A. Glaria, G. Varma, A. Protti, P. J. Blower, Bioconjugate Chem. 2011, 22, 455.
- 47J. Peng, Y. Sun, L. Zhao, Y. Wu, W. Feng, Y. Gao, F. Li, Biomaterials 2013, 34, 9535.
- 48L. Sandiford, A. Phinikaridou, A. Protti, L. K. Meszaros, X. Cui, Y. Yan, G. Frodsham, P. A. Williamson, N. Gaddum, R. M. Botnar, P. J. Blower, M. A. Green, R. T. de Rosales, ACS Nano 2013, 7, 500.
- 49S. Banerjee, G. Samuel, K. Kothari, P. R. Unni, H. D. Sarma, M. R. A. Pillai, Nucl. Med. Biol. 2001, 28, 205.
- 50B. Qiao, A. Wang, X. Yang, L. F. Allard, Z. Jiang, Y. Cui, J. Liu, J. Li, T. Zhang, Nat. Chem. 2011, 3, 634.
- 51B. Ravel, M. Newville, J. Synchrotron Radiat. 2005, 12, 537.
- 52K. Richard, R. Hume, E. Kaptein, J. P. Sanders, H. van Toor, W. W. De Herder, J. C. den Hollander, E. P. Krenning, T. J. Visser, J. Clin. Endocrinol. Metab. 1998, 83, 2868.
