Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid–Quantum-Dot Hybrid Particles
Correction(s) for this article
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Imaging Viral Behaviors in Mammalian Cells with Self-Assembled Capsid–Quantum-Dot Hybrid Particles
- F. Li,
- Z.-P. Zhang,
- J. Peng,
- Z.-Q. Cui,
- D.-W. Pang,
- K. Li,
- H.-P. Wei,
- Y.-F. Zhou,
- J.-K. Wen,
- X.-E. Zhang,
- Volume 5Issue 9Small
- First Published online: April 22, 2009
Feng Li
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Graduate School, Chinese Academy of Sciences No.19, Yuquan Road, Shijingshan, Beijing 100049 (China)
Search for more papers by this authorZhi-Ping Zhang
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Search for more papers by this authorJun Peng
College of Chemistry and Molecular Sciences, Wuhan University Luojiashan, Wuhan 430072 (China)
Search for more papers by this authorZong-Qiang Cui
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Search for more papers by this authorDai-Wen Pang
College of Chemistry and Molecular Sciences, Wuhan University Luojiashan, Wuhan 430072 (China)
Search for more papers by this authorKe Li
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Graduate School, Chinese Academy of Sciences No.19, Yuquan Road, Shijingshan, Beijing 100049 (China)
Search for more papers by this authorHong-Ping Wei
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Search for more papers by this authorYa-Feng Zhou
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Search for more papers by this authorJi-Kai Wen
School of Biosciences, The University of Birmingham Edgbaston, Birmingham B15 2TT (UK)
Search for more papers by this authorCorresponding Author
Xian-En Zhang
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China).Search for more papers by this authorFeng Li
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Graduate School, Chinese Academy of Sciences No.19, Yuquan Road, Shijingshan, Beijing 100049 (China)
Search for more papers by this authorZhi-Ping Zhang
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Search for more papers by this authorJun Peng
College of Chemistry and Molecular Sciences, Wuhan University Luojiashan, Wuhan 430072 (China)
Search for more papers by this authorZong-Qiang Cui
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Search for more papers by this authorDai-Wen Pang
College of Chemistry and Molecular Sciences, Wuhan University Luojiashan, Wuhan 430072 (China)
Search for more papers by this authorKe Li
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Graduate School, Chinese Academy of Sciences No.19, Yuquan Road, Shijingshan, Beijing 100049 (China)
Search for more papers by this authorHong-Ping Wei
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Search for more papers by this authorYa-Feng Zhou
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
Search for more papers by this authorJi-Kai Wen
School of Biosciences, The University of Birmingham Edgbaston, Birmingham B15 2TT (UK)
Search for more papers by this authorCorresponding Author
Xian-En Zhang
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China)
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071 (China).Search for more papers by this authorAbstract
Unique spectral properties of quantum dots (QDs) enable ultrasensitive and long-term biolabeling. Aiming to trace the infection, movement, and localization of viruses in living cells, QD-containing virus-like particles (VLPs) of simian virus 40 (SV40), termed SVLP-QDs, are constructed by in vitro self-assembly of the major capsid protein of SV40. SVLP-QDs show homogeneity in size (≈24 nm), similarity in spectral properties to unencapsidated QDs, and considerable stability. When incubated with living cells, SVLP-QDs are shown to enter the cells by caveolar endocytosis, travel along the microtubules, and accumulate in the endoplasmic reticulum. This process mimics the early infection steps of SV40. This is the first paradigm of imaging viral behaviors with encapsidated QDs in living cells. The method may provide a new alternative for various purposes, such as tracing viruses or viral components, targeted nanoparticle delivery, and probing of drug delivery.
Supporting Information
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