Research Article
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Multi-ink pattern generation by dip-pen nanolithography®
Jae-Won Jang,
Alexander Smetana, Paul Stiles,
Corresponding Author
Jae-Won Jang
NanoInk Inc., Skokie, Illinois
NanoInk Inc., 8025 Lamon Avenue, Skokie, IL 60077Search for more papers by this authorJae-Won Jang,
Alexander Smetana, Paul Stiles,
Corresponding Author
Jae-Won Jang
NanoInk Inc., Skokie, Illinois
NanoInk Inc., 8025 Lamon Avenue, Skokie, IL 60077Search for more papers by this authorAbstract
Multiplexed patterns of hydrogels and phospholipids with fluorescent dyes are accomplished by dip-pen nanolithography® (DPN®). For example, four different dye-labeled hydrogel dot arrays are DPN® patterned within 50×50 µm2 area, and two different dye-doped phospholipids dots and letters with less than 1 µm line-width are also DPN® patterned. We demonstrate that multi-ink patterns with precise alignment are able to be printed by DPN® within a micron-scale. Moreover, this multi-ink DPN® patterning methodology can be extended to delicate bio-materials printing in a subcellular scale with accurate positioning control. SCANNING 32: 24–29, 2010. © 2010 Wiley Periodicals, Inc.
References
- Agarwal G, Sowards LA, Naik RR, Stone MO: Dip-pen nanolithography in tapping mode. J Am Chem Soc 125, 580–583 (2003).
- Arnold M, Cavalcanti-Adam EA, Glass R, Blummel J, Eck W, et al.: Activation of integrin function by nanopatterned adhesive interfaces. Chem Phys Chem 5, 383–388 (2004).
- Basnar B, Weizmann Y, Cheglakov Z, Willner I: Synthesis of nanowires using dip-pen nanolithography and biocatalytic inks. Adv Mater 18, 713–718 (2006).
- Bruinink CM, Nijhuis CA, Peter M, Dordi B, Crespo-Biel O, et al.: Supramolecular microcontact printing and dip-pen nanolithography on molecular printboards. Chem-A Eur J 11, 3988–3996 (2005).
- Doh J, Irvine DJ: Immunological synapse arrays: patterned protein surfaces that modulate immunological synapse structure formation in T cells. Proc Natl Acad Sci USA 103, 5700–5705 (2006).
- Ginger DS, Zhang H, Mirkin CA: The evolution of dip-pen nanolithography. Angew Chem Int Ed Engl 43, 30–45 (2004).
- Groves JT: Spatial mutation of the T cell immunological synapse. Curr Opin Chem Biol 10, 544–550 (2006).
- Hampton JR, Dameron AA, Weiss PA: Double-ink dip-pen nanolithography studies elucidate molecular transport. J Am Chem Soc 128, 1648–1653 (2006).
- Hampton JR, Dameron AA, Weiss PS: Transport rates vary with deposition time in dip-pen nanolithography. J Phys Chem B 109, 23118–23120 (2005).
- Huang L, Braunschweig AB, Shim W, Qin L, Lim JK, et al.: Matrix-assisted dip-pen nanolithography and polymer pen lithography. Small in press (2009).
- Hyun J, Ahn SJ, Lee WK, Chilkoti A, Zauscher S: Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography. Nano Lett 2, 1203–1207 (2002).
- Jung H, Kulkarni R, Collier CP: Dip-pen nanolithography of reactive alkoxysilanes on glass. J Am Chem Soc 125, 12096–12097 (2003).
- Lenhert S, Sun P, Wang YH, Fuchs H, Mirkin CA: Massively parallel dip-pen nanolithography of heterogeneous supported phospholipid multilayer patterns. Small 3, 71–75 (2007).
- Li Y, Maynor BW, Liu J: Electrochemical AFM “dip-pen” nanolithography. J Am Chem Soc 123, 2105–2106 (2001).
- McKendry R, Huck WTS, Weeks B, Florini M, Abell C, et al.: Creating nanoscale patterns of dendrimers on silicon surfaces with dip-pen nanolithography. Nano Lett 2, 713–716 (2002).
- Mirkin CA: The power of the pen: development of massively parallel dip-pen nanolithography. ACS Nano 1, 79–83 (2007).
- Mossman KD, Campi G, Groves JT, Dustin ML: Altered TCR signaling from geometrically repatterned immunological synapses. Science 310, 1191–1193 (2005).
- Nafday OA, Haaheim JR, Villagran F: Site-specific dual Ink dip-pen nanolithographyTM. Scanning 31, 122–126 (2009).
- Nafday OA, Weeks BL: Relative humidity effects in dip-pen nanolithography of alkanethiol mixtures. Langmuir 22, 10912–10914 (2006).
- Nelson BA, King WP, Laracuente AR, Sheehan PE, Whitman LJ: Direct deposition of continuous metal nanostructures by thermal dip-pen nanolithography. Appl Phys Lett 88, 033104 (2006).
- Noy A, Miller AE, Klare JE, Weeks BL, Woods BW, et al.: Fabrication of luminescent nanostructures and polymer nanowires using dip-den nanolithography. Nano Lett 2, 109–112 (2002).
- Nyamjav D, Ivanisevic A: Alignment of long DNA molecules on templates generated via dip-pen nanolithography. Adv Mater 15, 1805–1809 (2003).
- Pena DJ, Raphael MP, Byers JM: “Dip-pen” nanolithography in registry with photolithography for biosensor development. Langmuir 19, 9028–9032 (2003).
- Salaita K, Lee SW, Wang XF, Huang L, Dellinger TM, et al.: Sub-100 nm, centimeter-scale, parallel dip-pen nanolithography. Small 1, 940–945 (2005).
- Salaita K, Wang YH, Fragala J, Vega RA, Liu C, et al.: Massively parallel dip-pen nanolithography with 55000-pen two-dimensional arrays. Angew Chem Int Ed Engl 45, 7220–7223 (2006).
- Salaita K, Wang YH, Mirkin CA: Applications of dip-pen nanolithography. Nat Nanotechnol 2, 145–155 (2007).
- Sekula S, Fuchs J, Weg-Remers S, Nagel P, Schuppler S, et al.: Multiplexed lipid dip-pen nanolithography on subcellular scales for the templating of functional proteins and cell culture. Small 4, 1785–1793 (2008).
- Sheehan PE, Whitman LJ: Thiol diffusion and the role of humidity in “dip-pen nanolithography”. Phys Rev Lett 88, 156104 (2002).
