In Vitro and In Vivo Inhibition of Glucocorticoid-induced Osteoporosis by the Hexane Extract of Poncirus trifoliata
Bo-Young Kim
Departments of Medical Genetics, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
These authors contributed equally to this work.Search for more papers by this authorHyung-Young Yoon
Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
These authors contributed equally to this work.Search for more papers by this authorSun-Il Yun
Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
Search for more papers by this authorEun-Rhan Woo
Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759 Republic of Korea
Search for more papers by this authorNan-Kyu Song
HL Genomics Co., Ltd, Yongin, 449-873 Republic of Korea
Search for more papers by this authorHyun-Gyu Kim
HL Genomics Co., Ltd, Yongin, 449-873 Republic of Korea
Search for more papers by this authorCorresponding Author
Seon-Yong Jeong
Departments of Medical Genetics, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
Professor Yoon-Sok Chung, Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, San 5, Wonchon-Dong, Yeongtong-Gu, Suwon 443-721, Republic of Korea. Professor Seon-Yong Jeong, Departments of Medical Genetics, School of Medicine, Ajou University, San 5, Wonchon-Dong, Yeongtong-Gu, Suwon 443-721, Republic of Korea.
E-mail: [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Yoon-Sok Chung
Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
Professor Yoon-Sok Chung, Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, San 5, Wonchon-Dong, Yeongtong-Gu, Suwon 443-721, Republic of Korea. Professor Seon-Yong Jeong, Departments of Medical Genetics, School of Medicine, Ajou University, San 5, Wonchon-Dong, Yeongtong-Gu, Suwon 443-721, Republic of Korea.
E-mail: [email protected]; [email protected]
Search for more papers by this authorBo-Young Kim
Departments of Medical Genetics, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
These authors contributed equally to this work.Search for more papers by this authorHyung-Young Yoon
Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
These authors contributed equally to this work.Search for more papers by this authorSun-Il Yun
Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
Search for more papers by this authorEun-Rhan Woo
Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759 Republic of Korea
Search for more papers by this authorNan-Kyu Song
HL Genomics Co., Ltd, Yongin, 449-873 Republic of Korea
Search for more papers by this authorHyun-Gyu Kim
HL Genomics Co., Ltd, Yongin, 449-873 Republic of Korea
Search for more papers by this authorCorresponding Author
Seon-Yong Jeong
Departments of Medical Genetics, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
Professor Yoon-Sok Chung, Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, San 5, Wonchon-Dong, Yeongtong-Gu, Suwon 443-721, Republic of Korea. Professor Seon-Yong Jeong, Departments of Medical Genetics, School of Medicine, Ajou University, San 5, Wonchon-Dong, Yeongtong-Gu, Suwon 443-721, Republic of Korea.
E-mail: [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Yoon-Sok Chung
Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, Suwon, 443-721 Republic of Korea
Professor Yoon-Sok Chung, Departments of Endocrinology and Metabolism, School of Medicine, Ajou University, San 5, Wonchon-Dong, Yeongtong-Gu, Suwon 443-721, Republic of Korea. Professor Seon-Yong Jeong, Departments of Medical Genetics, School of Medicine, Ajou University, San 5, Wonchon-Dong, Yeongtong-Gu, Suwon 443-721, Republic of Korea.
E-mail: [email protected]; [email protected]
Search for more papers by this authorAbstract
This study was performed to discover a novel herbal therapeutic for effective glucocorticoid-induced osteoporosis (GIO) treatment and further to clarify its molecular mechanism of action. Ethanol or methanol extracts of 68 edible Korean native plants were screened to find effective natural plant sources for the treatment of GIO, and Poncirus trifoliata (L.) (Rutaceae, PT) was selected as a final candidate because of its high inhibitory activity plus its novelty. The hexane extract of PT (PT-H) inhibited apoptotic cell death in dexamethasone-induced osteoblastic cell lines, C3H10T1/2 and MC3T3-E1. In vivo mouse results indicated that PT-H not only had an inhibitory effect on the bone loss caused by glucocorticoid, but also promoted bone formation. The molecular mechanisms behind the effect of PT-H on GIO were further clarified by screening of differentially expressed genes (DEGs) between dexamethasone (Dex)-induced osteoblastic cells with or without PT-H treatment. Finally, it was found that the expression level of AnxA6 in Dex-induced osteoblastic cells and prednisolone (PD)-treated GIO-model mice was significantly decreased by PT-H treatment. These findings suggest that PT-H has a strong in vitro and in vivo inhibitory effect on GIO, and decreased expression of AnxA6 may play a key role in this inhibition. Copyright © 2011 John Wiley & Sons, Ltd.
Supporting Information
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| Filename | Description |
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| Word document | Inhibitory effects of the ethanol or methanol extracts from 68 Korean plants against dexamethasone (Dex)-induced apoptosis of osteoblastic C3H10T1/2 cell line |
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