Multivariate Optimization for the Production of OSA-Starch Microcapsules Containing Clove and White Thyme Essential Oils: Antifungal Potential Against Penicillium digitatum
Corresponding Author
Estefania Júlia Dierings de Souza
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Correspondence:
Estefania Júlia Dierings de Souza ([email protected])
Search for more papers by this authorFelipe Nardo dos Santos
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorDianini Hüttner Kringel
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorJaqueline Ferreira de Souza
Laboratory of Technology and Development of Composites and Polymeric Materials (LaCoPol), Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorDanielle Tapia Bueno
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorAmanda Fonseca Leitzke
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorAndré Ricardo Fajardo
Laboratory of Technology and Development of Composites and Polymeric Materials (LaCoPol), Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorEliezer Avila Gandra
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorClaudio Martin Pereira de Pereira
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorAlvaro Renato Guerra Dias
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorElessandra da Rosa Zavareze
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorCorresponding Author
Estefania Júlia Dierings de Souza
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Correspondence:
Estefania Júlia Dierings de Souza ([email protected])
Search for more papers by this authorFelipe Nardo dos Santos
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorDianini Hüttner Kringel
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorJaqueline Ferreira de Souza
Laboratory of Technology and Development of Composites and Polymeric Materials (LaCoPol), Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorDanielle Tapia Bueno
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorAmanda Fonseca Leitzke
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorAndré Ricardo Fajardo
Laboratory of Technology and Development of Composites and Polymeric Materials (LaCoPol), Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorEliezer Avila Gandra
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorClaudio Martin Pereira de Pereira
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorAlvaro Renato Guerra Dias
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorElessandra da Rosa Zavareze
Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil
Search for more papers by this authorFunding: This work was supported by Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, 17/255100009126. Conselho Nacional de Desenvolvimento Científico e Tecnológico, 306378/2015-9. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, 001.
ABSTRACT
The objective of this study was to optimize the conditions for the production of microcapsules based on OSA-starch and clove (CEO) and white thyme (WTEO) essential oils (EOs) obtained by electrospraying. The antifungal activity against the fungus Penicillium digitatum was assessed using both the well diffusion method and volatile release action. The microcapsules were characterized by morphology, loading capacity (LC) presence of functional groups (FTIR), and thermal properties (TGA). Spherical microcapsules were formed in all treatments evaluated, with an average diameter ranging from 0.803 to 1.500 μm. The morphology of the materials was not affected by the variation of the solution parameters (ethanol: 20% and 30%, OSA-starch: 40% and 50%, CEO and WTEO, and EO concentration: 0.2% and 0.4%). The LC ranged from 30.6% to 97.9%, and TGA and FTIR analyses for all treatments confirmed the interaction of EOs with OSA-starch. Microcapsules from OSA-starch with CEO showed greater efficiency in reducing the mycelial growth of P. digitatum through the release of volatiles. These results serve as a basis for future studies to evaluate the technological applicability of these capsules in a food matrix or packaging material for controlling the fungal growth of P. digitatum.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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