Gallic acid-loaded electrospun cellulose acetate nanofibers as potential wound dressing materials
Corresponding Author
Patcharaporn Wutticharoenmongkol
Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Pathumthani, Thailand, 12120
Correspondence
Patcharaporn Wutticharoenmongkol, Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Pathumthani 12120, Thailand.
Email: [email protected]
Search for more papers by this authorPornchita Hannirojram
Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Pathumthani, Thailand, 12120
Search for more papers by this authorPimchanok Nuthong
Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Pathumthani, Thailand, 12120
Search for more papers by this authorCorresponding Author
Patcharaporn Wutticharoenmongkol
Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Pathumthani, Thailand, 12120
Correspondence
Patcharaporn Wutticharoenmongkol, Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Pathumthani 12120, Thailand.
Email: [email protected]
Search for more papers by this authorPornchita Hannirojram
Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Pathumthani, Thailand, 12120
Search for more papers by this authorPimchanok Nuthong
Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Pathumthani, Thailand, 12120
Search for more papers by this authorAbstract
Gallic acid (GA)–loaded cellulose acetate (CA) nanofiber mats with 10 to 40 wt.% GA contents (based on the weight of CA) were fabricated by electrospinning. The effects of GA contents and applied potential on the morphology and the average diameters of fibers were studied. The electrospun fiber mats containing 20 and 40 wt.% GA were investigated for their potential use as carrier of GA in wound dressing application. The GA-loaded CA films were prepared by solvent casting technique for use in comparative studies. Determination of the release characteristics of GA from the GA-loaded fiber mats and films was carried out by the total immersion and the transdermal diffusion through a pig skin method in acetate buffer solution (pH 5.5) or normal saline (pH 7.0) at either 32 or 37°C, respectively. In the total immersion method, the maximum amounts of the GA released from the fiber mats containing 20 and 40 wt.% GA in the acetate buffer were approximately 97% and 71% (based on the weight of initial GA), while those of the GA released into the normal saline were approximately 96% and 81%, respectively. Lower values were observed in the experiments of the transdermal diffusion through a pig skin method. The corresponding GA-loaded CA films showed the lower amounts of GA released into media. The as-loaded and the as-released GA remained its antioxidant activity as investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Lastly, the GA-loaded CA fiber mats exhibited antibacterial activity against Staphylococcus aureus, which showed the potential for use as wound dressing materials.
Supporting Information
| Filename | Description |
|---|---|
| pat4547-sup-0001-SI.docxWord 2007 document , 526 KB |
Table S1. Values of ln(t) and ln (Mt/M∞) for the release of GA from EF20GA in acetate buffer by total immersion method. Table S2. Values of ln(t) and ln (Mt/M∞) for the release of GA from CF20GA in acetate buffer by total immersion method. Table S3. Values of ln(t) and ln (Mt/M∞) for the release of GA from EF40GA in acetate buffer by total immersion method. Table S4. Values of ln(t) and ln (Mt/M∞) for the release of GA from CF40GA in acetate buffer by total immersion method. Table S5. Values of ln(t) and ln (Mt/M∞) for the release of GA from EF20GA in normal saline by total immersion method. Table S6. Values of ln(t) and ln (Mt/M∞) for the release of GA from CF20GA in normal saline by total immersion method. Table S7. Values of ln(t) and ln (Mt/M∞) for the release of GA from EF40GA in normal saline by total immersion method. Table S8. Values of ln(t) and ln (Mt/M∞) for the release of GA from CF40GA in normal saline by total immersion method. Table S9. Values of ln(t) and ln (Mt/M∞) for the release of GA from EF20GA in acetate buffer by transdermal diffusion through a pig skin method. Table S10. Values of ln(t) and ln (Mt/M∞) for the release of GA from CF20GA in acetate buffer by transdermal diffusion through a pig skin method. Table S11. Values of ln(t) and ln (Mt/M∞) for the release of GA from EF40GA in acetate buffer by transdermal diffusion through a pig skin method. Table S12. Values of ln(t) and ln (Mt/M∞) for the release of GA from CF40GA in acetate buffer by transdermal diffusion through a pig skin method. Table S13. Values of ln(t) and ln (Mt/M∞) for the release of GA from EF20GA in normal saline by transdermal diffusion through a pig skin method. Table S14. Values of ln(t) and ln (Mt/M∞) for the release of GA from CF20GA in normal saline by transdermal diffusion through a pig skin method. Table S15. Values of ln(t) and ln (Mt/M∞) for the release of GA from EF40GA in normal saline by transdermal diffusion through a pig skin method. Table S16. Values of ln(t) and ln (Mt/M∞) for the release of GA from CF40GA in normal saline by transdermal diffusion through a pig skin method. Figure S1. Plot of ln(t) and ln (Mt/M∞) for the release of GA from EF20GA in acetate buffer by total immersion method. Figure S2. Plot of ln(t) and ln (Mt/M∞) for the release of GA from CF20GA in acetate buffer by total immersion method. Figure S3. Plot of ln(t) and ln (Mt/M∞) for the release of GA from EF40GA in acetate buffer by total immersion method. Figure S4. Plot of ln(t) and ln (Mt/M∞) for the release of GA from CF40GA in acetate buffer by total immersion method. Figure S5. Plot of ln(t) and ln (Mt/M∞) for the release of GA from EF20GA in normal saline by total immersion method. Figure S6. Plot of ln(t) and ln (Mt/M∞) for the release of GA from CF20GA in normal saline by total immersion method. Figure S7. Plot of ln(t) and ln (Mt/M∞) for the release of GA from EF40GA in normal saline by total immersion method. Figure S8. Plot of ln(t) and ln (Mt/M∞) for the release of GA from CF40GA in normal saline by total immersion method. Figure S9. Plot of ln(t) and ln (Mt/M∞) for the release of GA from EF20GA in acetate buffer by transdermal diffusion through a pig skin method. Figure S10. Plot of ln(t) and ln (Mt/M∞) for the release of GA from CF20GA in acetate buffer by transdermal diffusion through a pig skin method. Figure S11. Plot of ln(t) and ln (Mt/M∞) for the release of GA from EF40GA in acetate buffer by transdermal diffusion through a pig skin method. Figure S12. Plot of ln(t) and ln (Mt/M∞) for the release of GA from CF40GA in acetate buffer by transdermal diffusion through a pig skin method. Figure S13. Plot of ln(t) and ln (Mt/M∞) for the release of GA from EF20GA in normal saline by transdermal diffusion through a pig skin method. Figure S14. Plot of ln(t) and ln (Mt/M∞) for the release of GA from CF20GA in normal saline by transdermal diffusion through a pig skin method. Figure S15. Plot of ln(t) and ln (Mt/M∞) for the release of GA from EF40GA in normal saline by transdermal diffusion through a pig skin method. Figure S16. Plot of ln(t) and ln (Mt/M∞) for the release of GA from CF40GA in normal saline by transdermal diffusion through a pig skin method. |
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