Comprehensive gene expression analysis of semaphorins in oral squamous cell carcinoma†
Aika Tanzawa
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Search for more papers by this authorCorresponding Author
Masashi Shiiba
Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan
Correspondence
Masashi Shiiba, Department of Medical Oncology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Japan.
Email: [email protected]
Search for more papers by this authorTomoaki Saito
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Search for more papers by this authorAtsushi Kasamatsu
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Search for more papers by this authorYosuke Endo-Sakamoto
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Search for more papers by this authorMasataka Sunohara
Department of Anatomy, School of Life Dentistry at Tokyo, Nippon Dental University, Chiyoda-ku, Japan
Search for more papers by this authorKatsuhiro Uzawa
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan
Search for more papers by this authorYuichi Takiguchi
Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan
Search for more papers by this authorHideki Tanzawa
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan
Search for more papers by this authorHiroshi Shirasawa
Department of Molecular Virology, Graduate School of Medicine, Chiba University, Chiba, Japan
Search for more papers by this authorAika Tanzawa
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Search for more papers by this authorCorresponding Author
Masashi Shiiba
Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan
Correspondence
Masashi Shiiba, Department of Medical Oncology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Japan.
Email: [email protected]
Search for more papers by this authorTomoaki Saito
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Search for more papers by this authorAtsushi Kasamatsu
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Search for more papers by this authorYosuke Endo-Sakamoto
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Search for more papers by this authorMasataka Sunohara
Department of Anatomy, School of Life Dentistry at Tokyo, Nippon Dental University, Chiyoda-ku, Japan
Search for more papers by this authorKatsuhiro Uzawa
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan
Search for more papers by this authorYuichi Takiguchi
Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan
Search for more papers by this authorHideki Tanzawa
Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan
Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan
Search for more papers by this authorHiroshi Shirasawa
Department of Molecular Virology, Graduate School of Medicine, Chiba University, Chiba, Japan
Search for more papers by this authorFunding information
This work was supported in part by JSPS KAKENHI Grant Number JP16K20559.
Abstract
Semaphorin family members (Semaphorins; SEMAs) consist of a large family that includes both secreted and membrane-associated proteins that are originally found to provide axon guidance in selected areas for neural development. The SEMAs have diverse biologic activities such as neuronal cellular migration, axon guidance, vasculogenesis, branching morphogenesis, and cardiac organogenesis. Recent studies have reported that SEMAs also play crucial roles in various carcinomas. However, the association and their role in oral squamous cell carcinoma (OSCC) are still poorly understood. In this study, we evaluated SEMAs mRNA expression in OSCC-derived cell lines using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis and revealed the altered gene expression profile of the SEMAs in OSCC cell lines compared with human normal oral keratinocytes. We found that tendency for up-regulation of SEMA3E, 4C, 5A, 6B, and 7A in OSCC-derived cell lines. Especially, SEMA3E and SEMA7A mRNA were significantly up-regulated in all OSCC-derived cell lines. On the other hands, SEMA3A, 3C, 3F, and 6A mRNA were significantly down-regulated in all OSCC-derived cell lines. The expression and role of SEMAs in OSCC depend on the forms and may provide novel insights and therapeutic target for OSCCs.
CONFLICT OF INTEREST
The authors declare that they have no competing interests.
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