Basic Science Article

Effects of the phosphodiesterase 5 inhibitor Tadalafil on bladder function in a rat model of partial bladder outlet obstruction

Corresponding Author

Yuko Kawai

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

Correspondence to: Ryohei Yoshinaga, Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, 14 Nishinosho-monguchi-cho, Minami-ku, Kisshoin, Kyoto, Japan.

E-mail: [email protected]

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Michiko Oka,

Michiko Oka

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

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Ryohei Yoshinaga,

Corresponding Author

Ryohei Yoshinaga

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

Correspondence to: Ryohei Yoshinaga, Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, 14 Nishinosho-monguchi-cho, Minami-ku, Kisshoin, Kyoto, Japan.

E-mail: [email protected]

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Chiaki Fuchikami,

Chiaki Fuchikami

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

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Tatsuya Oyama,

Tatsuya Oyama

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

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Yuko Kawai,

Corresponding Author

Yuko Kawai

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

Correspondence to: Ryohei Yoshinaga, Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, 14 Nishinosho-monguchi-cho, Minami-ku, Kisshoin, Kyoto, Japan.

E-mail: [email protected]

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Michiko Oka,

Michiko Oka

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

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Ryohei Yoshinaga,

Corresponding Author

Ryohei Yoshinaga

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

Correspondence to: Ryohei Yoshinaga, Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, 14 Nishinosho-monguchi-cho, Minami-ku, Kisshoin, Kyoto, Japan.

E-mail: [email protected]

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Chiaki Fuchikami,

Chiaki Fuchikami

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

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Tatsuya Oyama,

Tatsuya Oyama

Discovery Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan

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First published: 19 February 2015

https://doi.org/10.1002/nau.22732

Citations: 24

Karl-Erik Andersson led the peer-review process as the Associate Editor responsible for the paper.

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Abstract

Aims

To investigate the effect of the phosphodiesterase 5 (PDE5) inhibitor Tadalafil on bladder blood flow and bladder function in a rat model of partial bladder outlet obstruction (BOO).

Methods

Female 14–15-week-old Sprague–Dawley rats were divided into three groups. BOO was surgically induced in rats by placing a rubber ring around the urethra. BOO rats were administered daily oral Tadalafil (BOO-Tadalafil) or vehicle (BOO-Vehicle), while sham-operated animals were treated with vehicle (Sham). On the 14th day after surgery, micturition behavior was recorded for 24 hr by using a metabolic cage. On the 15th day after surgery, bladder blood flow and bladder weight were measured. The expression of PDE5 mRNA in the vesical and iliac arteries of intact rats was measured by reverse transcriptase-polymerase chain reaction.

Results

BOO led to a significant decrease in bladder blood flow and a significant increase in bladder weight. These changes were partially suppressed by Tadalafil treatment. The number of micturitions in the BOO group was significantly increased and the average micturition volume was significantly decreased, without affecting the total micturition volume. Repeated Tadalafil treatment markedly inhibited the increase in micturition frequency and the decrease in average micturition volume. PDE5 mRNA was expressed in the vesical and iliac arteries.

Conclusion

Tadalafil suppressed the reduction in bladder blood flow caused by BOO in rats and improved urinary function. This action of Tadalafil may contribute to its amelioration of bladder function. Neurourol. Urodynam. 35:444–449, 2016. © 2015 Wiley Periodicals, Inc.

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Citing Literature

Volume35, Issue4

April 2016

Pages 444-449

Effects of the phosphodiesterase 5 inhibitor Tadalafil on bladder function in a rat model of partial bladder outlet obstruction

Abstract

Aims

Methods

Results

Conclusion

REFERENCES

Citing Literature

References

Information

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Effects of the phosphodiesterase 5 inhibitor Tadalafil on bladder function in a rat model of partial bladder outlet obstruction

Abstract

Aims

Methods

Results

Conclusion

REFERENCES

Citing Literature

References

Related

Information