Expression of the low-affinity NGF receptor during human muscle development, regeneration, and in tissue culture
Corresponding Author
Dr. Pierluigi Baron MD, PhD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Istituto di Clinica Neurologica, Università di Milano, Via F. Sforza 35, 20122 Milano, ItalySearch for more papers by this authorDr. Elio Scarpini MD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Search for more papers by this authorDr. Gianni Meola MD
Second Division of Neurology, San Donate Hospital, Milan, Italy
Search for more papers by this authorDr. Ignazio Santilli MD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Search for more papers by this authorDr. Giancarlo Conti MD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Search for more papers by this authorDr. David Pleasure MD
Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania
Search for more papers by this authorDr. Guglielmo Scarlato MD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Search for more papers by this authorCorresponding Author
Dr. Pierluigi Baron MD, PhD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Istituto di Clinica Neurologica, Università di Milano, Via F. Sforza 35, 20122 Milano, ItalySearch for more papers by this authorDr. Elio Scarpini MD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Search for more papers by this authorDr. Gianni Meola MD
Second Division of Neurology, San Donate Hospital, Milan, Italy
Search for more papers by this authorDr. Ignazio Santilli MD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Search for more papers by this authorDr. Giancarlo Conti MD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Search for more papers by this authorDr. David Pleasure MD
Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania
Search for more papers by this authorDr. Guglielmo Scarlato MD
Institute of Clinical Neurology, Dino Ferrari Center, University of Milan, Milan, Italy
Search for more papers by this authorAbstract
The expression of the low-affinity NGF receptor (LNGFR) during human muscle development, regeneration, and in tissue culture was analyzed using a murine monoclonal antibody to human LNGFR (MAb ME 20.4). Muscle cells from 12–22-week fetuses stained strongly for LNGFR. In adult normal muscle, only intramuscular nerve endings showed immunoreactivity with MAb ME 20.4, but no staining was detected in muscle fibers. In Duchenne muscular dystrophy, immunohistologically demonstrable LNGFR was present in regenerating muscle fibers. In these fibers, LNGFR gene expression was also demonstrated at the transcriptional level using in situ hybridization with riboprobes coding with human LNGFR. Cultures from human fetal and adult muscle were studied by double label indirect immunofluorescence microscopy with MAb ME 20.4 and antisera against human fetal myosin. Most myosin-positive cells, both at the myoblast and myotube stages, displayed surface LNGFR immunostaining. In cells from fetal muscle, LNGFR was detected during the first 2 weeks in vitro, whereas in cells from adult muscle the expression of LNGFR was observed for up to 7 weeks. These findings suggest a potential involvement of LNGFR in human muscle development and regeneration. © 1994 John Wiley & Sons, Inc.
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