Article
Full Access
Isolation and characterization of sarcoplasmic reticulum from normal and dystrophic chicken
Dr. Richard M. Kawamoto PhD,
Dr. Ronald J. Baskin PhD,
Dr. Richard M. Kawamoto PhD
The Department of Zoology, University of California at Davis, Davis, CA
Search for more papers by this authorCorresponding Author
Dr. Ronald J. Baskin PhD
The Department of Zoology, University of California at Davis, Davis, CA
Department of Zoology, University of California at Davis, Davis, CA 95616Search for more papers by this authorDr. Richard M. Kawamoto PhD,
Dr. Ronald J. Baskin PhD,
Dr. Richard M. Kawamoto PhD
The Department of Zoology, University of California at Davis, Davis, CA
Search for more papers by this authorCorresponding Author
Dr. Ronald J. Baskin PhD
The Department of Zoology, University of California at Davis, Davis, CA
Department of Zoology, University of California at Davis, Davis, CA 95616Search for more papers by this authorAbstract
Purified sarcoplasmic reticulum vesicles from normal and dystrophic chicken skeletal muscle have been isolated by a procedure employing pressure disruption of a microsomal suspension. The dystrophic sarcoplasmic reticulum (SR) exhibited reduced Ca+ + transport and phosphoenzyme formation, but the Ca + + -ATPase activity was normal. Normal and dystrophic SR showed similar lipid profiles, except for a significant increase in free fatty acids in the dystrophic SR. Investigations involving the interaction of oleic acid with normal SR showed fatty acids can induce conditions similar to those found in dystrophic SR.
References
- 1
Asmundson VS,
Julian LM:
Inherited muscle abnormality in the domestic fowl.
J Hered
47: 248–252
1956.
10.1093/oxfordjournals.jhered.a106655 Google Scholar
- 2 Boland R, Martonosi A, Tillack TW: Developmental changes in the composition and function of sarcoplasmic reticulum. J Biol Chem 249: 612–623 1974.
- 3 Bradford M: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254 1976.
- 4 Brandt NR, Caswell AH, Brunschwig J-P: ATP-energized Ca++ pump in isolated transverse tubules of skeletal muscle. J Biol Chem 255: 6290–6298 1980.
- 5 Bryant SH: Myotonia in the goat. Ann NY Acad Sci 317: 314–325 1979.
- 6 Caswell AH, Lau YH, Brunschwig J-P: Ouabain-binding vesicles from skeletal muscle. Arch Biochem Biophys 176: 417–430 1976.
- 7 Chio LF, Peterson DW, Kratzer FH: Lipid composition and synthesis in the muscles of normal and dystrophic chickens. Can J Biochem 50: 1267–1272 1972.
- 8 Crowe LE, Baskin RJ: Stereologic analysis of dystrophic chicken muscle. J Ultrastruct Res 62: 147–154 1978.
- 9 Cullis PR, DeKruijff B: Polymorphic phase behavior of lipid mixtures as detected by 31P NMR. Biochim Biophys Acta 507: 207–218 1978.
- 10 Cullis PR, DeKruijff B: Lipid polymorphism and the functional roles of lipids in biological membranes. Biochim Biophys Acta 559: 399–420 1979.
- 11 Cullis PR, Hope MJ: The bilayer stabilizing role of sphingomyelin in the presence of cholesterol—31P NMR study. Biochim Biophys Acta 597: 533–542 1980.
- 12 Deamer DW, Boatman DE: An enzymatically driven membrane reconstitution from solubilized components. J Cell Biol 84: 461–467 1980.
- 13 Fernandez JL, Rosemblatt M, Hidalgo C: Highly purified sarcoplasmic reticulum vesicles are devoid of Ca++-independent (basal) ATPase activity. Biochim Biophys Acta 599: 552–568 1980.
- 14 Garaham PJ, Pouchan MI, Rega AF: Potassium activated phosphatase from human red blood cells. The mechanism of potassium activation. J Physiol (Lond) 202: 305–327 1969.
- 15 Hanna SD, Baskin RJ: Calcium transport and phosphoenzyme formation in sarcoplasmic reticulum. Biochem Med 17: 300–309 1977.
- 16 Hanna SD, Baskin RJ: Comparison of the (Ca++ + Mg+ +)-ATPase proteins from normal and dystrophic chicken sarcoplasmic reticulum. Biochim Biophys Acta 540: 144–150 1978.
- 17 Hanna SD, Kawamoto R, McNamee M, Baskin RJ: Enzymatic activity of dystrophic chicken sarcoplasmic reticulum. Biochim Biophys Acta 643: 41–54 1981.
- 18 Hsu QS, Kaldor G: Studies on the lipid composition of the fragmented sarcoplasmic reticulum of normal and dystrophic chicken. Proc Soc Exp Biol Med 138: 733–737 1971.
- 19 Kates M: Lipid extraction procedures, in TS Work, E Work, (eds): Techniques of Lipidology, Chapter 3. New York, American Elsevier, 1972, pp 351–352.
- 20 Kawamoto RM, Baskin RJ: Isolation and characterization of SR and T-tubules from normal and dystrophic chicken muscle. J Cell Biol 91: 257a, 1981.
- 21 Kidd PM, Yasumura T: T-system abnormalities in differentiating skeletal fibers of dystrophic chicken. Muscle Nerve 5: 472–478 1982.
- 22 Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 1970.
- 23 Lau YH, Caswell AH, Brunschwig J-P: Isolation of transverse tubules by fractionation of triad junctions of skeletal muscle. J Biol Chem 252: 5565–5574 1977.
- 24 Lau YH, Caswell AH, Brunschwig J-P, Baerwald RJ, Garcia M: Lipid analysis and freeze fracture studies on isolated transverse tubules and sarcoplasmic reticulum subfractions of skeletal muscle. J Biol Chem 254: 540–546 1979.
- 25 Malouf N, Sommer J: Chicken dystrophy. The geometry of the transverse tubules. Am J Pathol 84: 299–316 1976.
- 26 Meissner G, Fleischer S: Characterization of sarcoplasmic reticulum from skeletal muscle. Biochim Biophys Acta 241: 356–378 1971.
- 27 Mrak RE, Fleischer S: Lipid composition of sarcoplasmic reticulum from mice with muscular dystrophy. Muscle Nerve 5: 439–446 1982.
- 28 Owens K, Hughes BP: Lipids of dystrophic and normal mouse muscle: whole tissue and particulate fractions. J Lipid Res 11: 486–495 1970.
- 29 Polinski WJ, Rall JA: Mechanics and energetics of muscle contraction in normal and dystrophic chickens. Am J Physiol 242: C19–C24, 1982.
- 30 Rodan SB, Hintz RL, Sha'afi RI, Rodan GA: The activity of membrane bound enzymes in muscular dystrophic chicks. Nature 252: 589–591 1974.
- 31 Rosemblatt M, Hidalgo C, Vergara C, Ikemoto N: Immunological and biochemical properties of transverse tubule membranes isolated from rabbit skeletal muscle. J Biol Chem 256: 8140–8148 1981.
- 32 Rushbrook JI, Yuan AI, Stacher A: Two major allelic forms of myosin light chain-1 in strains of normal and dystrophic chicken. Muscle Nerve 5: 505–514 1982.
- 33 Scales D, Sabbadini R, Inesi G: The involvement of sarcotubular membranes in genetic muscular dystrophy. Biochim Biophys Acta 465: 535–549 1977.
- 34 Scales DJ, Sabbadini RA: Microsomal T-system—stereological analysis of purified microsomes derived from normal and dystrophic skeletal muscle. J Cell Biol 83: 33–46 1979.
- 35 Sreter FA, Martonosi A, Gergely J: Sarcoplasmic reticulum in the dystrophic mouse and chicken. Fed Proc 23: 530, 1964.
- 36 Stewart P, Werstiuk E, Vickers J, Rathbone M: Elevated cholesterol in tissues of chicken embryos with hereditary myotonic muscular dystrophy. Exp Neurol 57: 475–485 1977.
- 37 Sumnicht GE, Sabbadini RA: Lipid composition of transverse tubular membranes from normal and dystrophic skeletal muscle. Arch Biochem Biophys 215: 628–637 1982.
- 38 Verjovski-Almeida S, Inesi G: Rapid kinetics of calcium ion transport and ATPase activity in the sarcoplasmic reticulum of dystrophic muscle. Biochim Biophys Acta 558: 119–125 1979.
- 39 Warren G, Toon P, Birdsall N, Lee A, Metcalfe J: Reconstitution of a calcium pump using defined membrane components. Proc Natl Acad Sci USA 71: 622–626 1974.
- 40
Weber K,
Osborn M:
Proteins and SOS molecular weight determination on polyacrylamide gels and related procedures,
in H Neurath,
R Hill (eds):
The Proteins, Chapter 3, Vol. 1.
Orlando, FL, Academic Press,
1975,
pp 179–233.
10.1016/B978-0-12-516301-9.50007-3 Google Scholar
- 41 Wilson BW, Randall WR, Patterson GT, Entrikin RK: Major physiologic and histochemical characteristics of inherited dystrophy of the chicken. Ann NY Acad Sci 317: 224–246 1979.
Citing Literature
