Mechanisms underlying immobilization-induced muscle pain in rats
Satoshi Oga MS
Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Department of Rehabilitation, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan
Search for more papers by this authorKyo Goto PhD
Department of Rehabilitation, Nagasaki Memorial Hospital, Nagasaki, Japan
Search for more papers by this authorCorresponding Author
Junya Sakamoto PhD
Department of Physical Therapy Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Correspondence
Junya Sakamoto PhD, Department of Physical Therapy Science, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8520, Japan.
Email: [email protected]
Search for more papers by this authorYuichiro Honda PhD
Department of Rehabilitation, Nagasaki University Hospital, Nagasaki, Japan
Search for more papers by this authorRyo Sasaki MS
Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Department of Rehabilitation, Juzenkai Hospital, Nagasaki, Japan
Search for more papers by this authorKumiko Ishikawa MS
Department of Rehabilitation, Nagasaki University Hospital, Nagasaki, Japan
Search for more papers by this authorHideki Kataoka PhD
Department of Rehabilitation, Nagasaki Memorial Hospital, Nagasaki, Japan
Search for more papers by this authorJiro Nakano PhD
Department of Physical Therapy Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Search for more papers by this authorTomoki Origuchi MD
Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Search for more papers by this authorMinoru Okita PhD
Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Search for more papers by this authorSatoshi Oga MS
Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Department of Rehabilitation, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan
Search for more papers by this authorKyo Goto PhD
Department of Rehabilitation, Nagasaki Memorial Hospital, Nagasaki, Japan
Search for more papers by this authorCorresponding Author
Junya Sakamoto PhD
Department of Physical Therapy Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Correspondence
Junya Sakamoto PhD, Department of Physical Therapy Science, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8520, Japan.
Email: [email protected]
Search for more papers by this authorYuichiro Honda PhD
Department of Rehabilitation, Nagasaki University Hospital, Nagasaki, Japan
Search for more papers by this authorRyo Sasaki MS
Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Department of Rehabilitation, Juzenkai Hospital, Nagasaki, Japan
Search for more papers by this authorKumiko Ishikawa MS
Department of Rehabilitation, Nagasaki University Hospital, Nagasaki, Japan
Search for more papers by this authorHideki Kataoka PhD
Department of Rehabilitation, Nagasaki Memorial Hospital, Nagasaki, Japan
Search for more papers by this authorJiro Nakano PhD
Department of Physical Therapy Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Search for more papers by this authorTomoki Origuchi MD
Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Search for more papers by this authorMinoru Okita PhD
Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Search for more papers by this authorFunding information: JSPS KAKENHI, Grant/Award Number: JP18K17751
Abstract
Introduction
We investigated the mechanisms underlying immobilization-induced muscle pain in rats.
Methods
In rat skeletal muscle, pressure pain threshold (PPT) of the gastrocnemius muscle was measured, and nerve growth factor (NGF) level, peripheral nerve fiber density, macrophage number, and interleukin-1β (IL-1β) mRNA expression were examined. An NGF receptor inhibitor was injected intramuscularly to assess the relationship between PPT and NGF levels.
Results
Immobilization resulted in a decrease in PPT and increases in NGF level, C-fiber density, M1 macrophage number, and IL-1β mRNA expression. Injection of NGF receptor inhibitor reversed the decrease in PPT.
Discussion
NGF upregulation may be a major contributor to immobilization-induced muscle pain. The increases in C-fiber density, M1 macrophage number, and IL-1β mRNA expression may be related to immobilization-induced muscle pain.
5 CONFLICT OF INTEREST
The authors declare no potential conflicts of interest.
Supporting Information
| Filename | Description |
|---|---|
| mus26840-sup-0001-TableS1.docxWord 2007 document , 22.8 KB | Supplemental Table 1 Sequences of primers used in real-time RT PCR |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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