Quantitative T2 mapping of the mouse heart by segmented MLEV phase-cycled T2 preparation
Bram F. Coolen
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Department of Radiology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorFrank F.J. Simonis
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
University Medical Center Utrecht, Utrecht, The Netherlands
Search for more papers by this authorTessa Geelen
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Search for more papers by this authorRik P.M. Moonen
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Search for more papers by this authorFatih Arslan
University Medical Center Utrecht, Utrecht, The Netherlands
Search for more papers by this authorLeonie E.M. Paulis
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
Search for more papers by this authorKlaas Nicolay
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Search for more papers by this authorCorresponding Author
Gustav J. Strijkers
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Correspondence to: Gustav J. Strijkers, Ph.D., Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600MB Eindhoven, the Netherlands. E-mail: [email protected]Search for more papers by this authorBram F. Coolen
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Department of Radiology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorFrank F.J. Simonis
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
University Medical Center Utrecht, Utrecht, The Netherlands
Search for more papers by this authorTessa Geelen
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Search for more papers by this authorRik P.M. Moonen
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Search for more papers by this authorFatih Arslan
University Medical Center Utrecht, Utrecht, The Netherlands
Search for more papers by this authorLeonie E.M. Paulis
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
Search for more papers by this authorKlaas Nicolay
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Search for more papers by this authorCorresponding Author
Gustav J. Strijkers
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
Correspondence to: Gustav J. Strijkers, Ph.D., Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600MB Eindhoven, the Netherlands. E-mail: [email protected]Search for more papers by this authorAbstract
Purpose
A high-quality, reproducible, multi-slice T2-mapping protocol for the mouse heart is presented.
Methods
A T2-prepared sequence with composite 90° and 180° radiofrequency pulses in a segmented MLEV phase cycling scheme was developed. The T2-mapping protocol was optimized using simulations and evaluated with phantoms.
Results
Repeatability for determination of myocardial T2 values was assessed in vivo in n = 5 healthy mice on 2 different days. The average baseline T2 of the left ventricular myocardium was 22.5 ± 1.7 ms. The repeatability coefficient for R2 = 1/T2 for measurements at different days was ΔR2 = 6.3 s−1. Subsequently, T2 mapping was applied in comparison to late-gadolinium-enhancement (LGE) imaging, to assess 1-day-old ischemia/reperfusion (IR) myocardial injury in n = 8 mice. T2 in the infarcts was significantly higher than in remote tissue, whereas remote tissue was not significantly different from baseline. Infarct sizes based on T2 versus LGE showed strong correlation. To assess the time-course of T2 changes in the infarcts, T2 mapping was performed at day 1, 3, and 7 after IR injury in a separate group of mice (n = 16). T2 was highest at day 3, in agreement with the expected time course of edema formation and resolution after myocardial infarction.
Conclusion
T2 prepared imaging provides high quality reproducible T2 maps of healthy and diseased mouse myocardium. Magn Reson Med 72:409–417, 2014. © 2013 Wiley Periodicals, Inc.
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