Water- and fat-suppressed proton projection MRI (WASPI) of rat femur bone
Corresponding Author
Yaotang Wu
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, Boston, Massachusetts, USA
Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, 300 Longwood Ave., Boston, MA 02115===Search for more papers by this authorGuangping Dai
Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorJerome L. Ackerman
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, Boston, Massachusetts, USA
Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorMelvin J. Glimcher
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, Boston, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorBrian D. Snyder
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, Boston, Massachusetts, USA
Orthopedic Biomechanics Laboratory, Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorAra Nazarian
Orthopedic Biomechanics Laboratory, Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorDavid A. Chesler
Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorCorresponding Author
Yaotang Wu
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, Boston, Massachusetts, USA
Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, 300 Longwood Ave., Boston, MA 02115===Search for more papers by this authorGuangping Dai
Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorJerome L. Ackerman
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, Boston, Massachusetts, USA
Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorMelvin J. Glimcher
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, Boston, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorBrian D. Snyder
Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Children's Hospital, Boston, Massachusetts, USA
Orthopedic Biomechanics Laboratory, Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorAra Nazarian
Orthopedic Biomechanics Laboratory, Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorDavid A. Chesler
Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorAbstract
Investigators often study rats by μCT to investigate the pathogenesis and treatment of skeletal disorders in humans. However, μCT measurements provide information only on bone mineral content and not the solid matrix. CT scans are often carried out on cancellous bone, which contains a significant volume of marrow cells, stroma, water, and fat, and thus the apparent bone mineral density (BMD) does not reflect the mineral density within the matrix, where the mineral crystals are localized. Water- and fat-suppressed solid-state proton projection imaging (WASPI) was utilized in this study to image the solid matrix content (collagen, tightly bound water, and other immobile molecules) of rat femur specimens, and meet the challenges of small sample size and demanding submillimeter resolution. A method is introduced to recover the central region of k-space, which is always lost in the receiver dead time when free induction decays (FIDs) are acquired. With this approach, points near the k-space origin are sampled under a small number of radial projections at reduced gradient strength. The typical scan time for the current WASPI experiments was 2 hr. Proton solid-matrix images of rat femurs with 0.4-mm resolution and 12-mm field of view (FOV) were obtained. This method provides a noninvasive means of studying bone matrix in small animals. Magn Reson Med 57:554–567, 2007. © 2007 Wiley-Liss, Inc.
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