Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection
Corresponding Author
Tomoharu Osada
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii
Department of Regenerative and Developmental Biology, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.Search for more papers by this authorAtsushi Toyoda
Human Genome Research Group, RIKEN Genomic Science Center, Tsurumi-ku. Yokohama, Kanagawa, Japan
Search for more papers by this authorStefan Moisyadi
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii
Search for more papers by this authorHidenori Akutsu
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii
Search for more papers by this authorMasahira Hattori
Human Genome Research Group, RIKEN Genomic Science Center, Tsurumi-ku. Yokohama, Kanagawa, Japan
Search for more papers by this authorYoshiyuki Sakaki
Human Genome Research Group, RIKEN Genomic Science Center, Tsurumi-ku. Yokohama, Kanagawa, Japan
Search for more papers by this authorRyuzo Yanagimachi
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii
Search for more papers by this authorCorresponding Author
Tomoharu Osada
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii
Department of Regenerative and Developmental Biology, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.Search for more papers by this authorAtsushi Toyoda
Human Genome Research Group, RIKEN Genomic Science Center, Tsurumi-ku. Yokohama, Kanagawa, Japan
Search for more papers by this authorStefan Moisyadi
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii
Search for more papers by this authorHidenori Akutsu
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii
Search for more papers by this authorMasahira Hattori
Human Genome Research Group, RIKEN Genomic Science Center, Tsurumi-ku. Yokohama, Kanagawa, Japan
Search for more papers by this authorYoshiyuki Sakaki
Human Genome Research Group, RIKEN Genomic Science Center, Tsurumi-ku. Yokohama, Kanagawa, Japan
Search for more papers by this authorRyuzo Yanagimachi
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii
Search for more papers by this authorAbstract
We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology. Mol. Reprod. Dev. © 2005 Wiley-Liss, Inc.
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