Membrane molecules involved in adhesion properties of cultured sertoli cells
Corresponding Author
Dr. Bianca M. Zani
Istituto di Istologia ed Embriologia, Università degli Studi di Roma “La Sapienza,” Rome, Italy
Istituto di Istologia ed Embriologia generale, Via A. Scarpa 14, 00161 Roma, ItalySearch for more papers by this authorElio Ziparo
Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università di L'Aquila, L'Aquila, Italy
Search for more papers by this authorMario A. Russo
Istituto di Istologia ed Embriologia, Università degli Studi di Roma “La Sapienza,” Rome, Italy
Search for more papers by this authorAntonio Filippini
Istituto di Istologia ed Embriologia, Università degli Studi di Roma “La Sapienza,” Rome, Italy
Search for more papers by this authorMario Stefanini
Istituto di Istologia ed Embriologia, Università degli Studi di Roma “La Sapienza,” Rome, Italy
Search for more papers by this authorCorresponding Author
Dr. Bianca M. Zani
Istituto di Istologia ed Embriologia, Università degli Studi di Roma “La Sapienza,” Rome, Italy
Istituto di Istologia ed Embriologia generale, Via A. Scarpa 14, 00161 Roma, ItalySearch for more papers by this authorElio Ziparo
Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università di L'Aquila, L'Aquila, Italy
Search for more papers by this authorMario A. Russo
Istituto di Istologia ed Embriologia, Università degli Studi di Roma “La Sapienza,” Rome, Italy
Search for more papers by this authorAntonio Filippini
Istituto di Istologia ed Embriologia, Università degli Studi di Roma “La Sapienza,” Rome, Italy
Search for more papers by this authorMario Stefanini
Istituto di Istologia ed Embriologia, Università degli Studi di Roma “La Sapienza,” Rome, Italy
Search for more papers by this authorAbstract
Membrane components involved in adhesion properties of cultured Sertoli cells have been studied by a combination of immunological and biochemical methods. An antiserum prepared against Sertoli cells induced reversible rounding and detachment of the cells from the culture dishes. The cell surface morphology during detachment was studied by scanning electron microscopy and indirect immunofluorescence. A Triton soluble fraction of crude membrane preparations inhibited the antibody-induced detachment. The antibodies recognized a restricted number of membrane glycoproteins [detectable as prominent bands on Sodium dodecylsulphate polyacrilamide gel electrophoresis (SDS-PAGE), Mr 170, 140, 80, and 48K] both in the Triton soluble fraction of crude membrane preparation and on intact Sertoli cells. The data suggest that the molecules involved in adhesion properties of cultured Sertoli cells are integral membrane glycoproteins exposing antigenic determinants at the cell surface.
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