An endocytotic process in Xenopus eggs was studied by direct visualization of the fluorescent tracer lucifer yellow CH in 1-μm-thick sections. Eggs fixed 3 min after activation show small fluorescent vesicles in the cortex; those fixed after 30 min show mainly large vesicles. This process is related to membrane recycling after cortical granule exocytosis and can account for the observed decrease of the effective membrane capacity.

References

Carron CP, Longo FJ (1984): Pinocytosis in fertilized sea urchin (Arbacia punctulata) eggs. J Exp Zool 231: 413–422.
10.1002/jez.1402310315
CAS Web of Science® Google Scholar
Charbonneau M, Picheral B (1983): Early events in Anuran Amphibian fertilization: An ultrastructural study of changes occurring in the course of monospermic fertilization and artificial activation. Dev Growth Differ 25: 23–37.
10.1111/j.1440-169X.1983.00023.x
Web of Science® Google Scholar
Donovan M, Hart NH (1982): Uptake of ferritin by mosaic egg surface of Brachydanio. J Exp Zool 223: 299–304.
10.1002/jez.1402230312
CAS PubMed Web of Science® Google Scholar
Fisher G, Rebhun LI (1983): Sea urchin egg cortical granule exocytosis is followed by a burst of membrane retrieval via uptake into coated vesicles. Dev Biol 99: 456–472.
10.1016/0012-1606(83)90295-6
CAS PubMed Web of Science® Google Scholar
Jaffe LA, Schlichter LC (1985): Fertilization-induced ionic conductances in eggs of the frog, Rana pipiens. J Physiol 358: 299–319.
10.1113/jphysiol.1985.sp015552
PubMed Web of Science® Google Scholar
Miller DK, Griffiths E, Lenard J, Firestone RA (1983): Cell killing by lysosomotropic detergents. J Cell Biol 97: 1841–1851.
10.1083/jcb.97.6.1841
CAS PubMed Web of Science® Google Scholar
Peres A, Bernardini G (1984): Conductance and capacity of the Xenopus egg membrane during activation. In: “8th Int Biophysics Cong Abstracts.” p 221.
Google Scholar
Peres A, Bernardini G (1985): The effective membrane capacity of Xenopus eggs: its relations with membrane conductance and cortical granule exocytosis. Pflugers Arch 404: 266–272.
10.1007/BF00581249
PubMed Web of Science® Google Scholar
Riezman H (1985): Endocytosis in yeast: several of the yeast secretory mutants are defective in endocytosis. Cell 40: 1001–1009.
10.1016/0092-8674(85)90360-5
CAS PubMed Web of Science® Google Scholar
Sardet C (1984): The ultrastructure of the sea urchin egg cortex isolated before and after fertilization. Dev Biol 105: 196–210.
10.1016/0012-1606(84)90275-6
CAS PubMed Web of Science® Google Scholar
Schlichter LC, Jaffe LA (1984): Fertilization-induced chloride and potassium conductances in eggs of the frog, Rana pipiens. Biophys J 45: 23a.
Google Scholar
Stewart WW (1978): Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell 14: 741–759.
10.1016/0092-8674(78)90256-8
CAS PubMed Web of Science® Google Scholar
Stewart WW (1981): Lucifer dyes-highly fluorescent dyes for biological tracing. Nature 292: 17–21.
10.1038/292017a0
CAS PubMed Web of Science® Google Scholar

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Volume14, Issue2

June 1986

Pages 123-127

The decrease of Xenopus egg membrane capacity during activation might be due to endocytosis

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The decrease of Xenopus egg membrane capacity during activation might be due to endocytosis

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