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19-Norandrostenedione (4-estrene-3,17-dione) Inhibits Porcine Oocyte Maturation In Vitro

Corresponding Author

Dr. S. A. J. Daniel

MRC Group in Reproductive Biology, University of Western Ontario, London, Ontario, Canada

Departments of Obstetrics and Gynaecology, University of Western Ontario, London, Ontario, Canada

MRC Group in Reproductive Biology, Department of Obstetrics and Gynaecology, University of Western Ontario, 339 Windermere Rd., London, Ontario, Canada N6A 5A5Search for more papers by this author

M. W. Khalil,

M. W. Khalil

MRC Group in Reproductive Biology, University of Western Ontario, London, Ontario, Canada

Departments of Obstetrics and Gynaecology, University of Western Ontario, London, Ontario, Canada

Department of Biochemistry, University of Western Ontario, London, Ontario, Canada

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D. T. Armstrong,

D. T. Armstrong

MRC Group in Reproductive Biology, University of Western Ontario, London, Ontario, Canada

Departments of Obstetrics and Gynaecology, University of Western Ontario, London, Ontario, Canada

Department of Physiology, University of Western Ontario, London, Ontario, Canada

D. T. Armstrong is a career investigator of the MRC (Canada)

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Dr. S. A. J. Daniel,

Corresponding Author

Dr. S. A. J. Daniel

MRC Group in Reproductive Biology, University of Western Ontario, London, Ontario, Canada

Departments of Obstetrics and Gynaecology, University of Western Ontario, London, Ontario, Canada

MRC Group in Reproductive Biology, Department of Obstetrics and Gynaecology, University of Western Ontario, 339 Windermere Rd., London, Ontario, Canada N6A 5A5Search for more papers by this author

M. W. Khalil,

M. W. Khalil

MRC Group in Reproductive Biology, University of Western Ontario, London, Ontario, Canada

Departments of Obstetrics and Gynaecology, University of Western Ontario, London, Ontario, Canada

Department of Biochemistry, University of Western Ontario, London, Ontario, Canada

Search for more papers by this author

D. T. Armstrong,

D. T. Armstrong

MRC Group in Reproductive Biology, University of Western Ontario, London, Ontario, Canada

Departments of Obstetrics and Gynaecology, University of Western Ontario, London, Ontario, Canada

Department of Physiology, University of Western Ontario, London, Ontario, Canada

D. T. Armstrong is a career investigator of the MRC (Canada)

Search for more papers by this author

First published: February 1986

https://doi.org/10.1002/mrd.1120130210

Citations: 7

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Abstract

Recent work has shown that 19-norandrostenedione is a major steroidal component of porcine follicular fluid; however, little is known about its role(s) in the regulation of follicular function. This study was designed to examine the effect of 19-norandrostenedione on porcine oocyte maturation in vitro. Oocyte-cumulus complexes were isolated from medium (3–6-mm diameter)-sized prepubertal pig follicles and incubated for 12 h in medium with or without dibutyryl cyclic AMP ((Bu)₂cAMP, 1 mM) with or without testosterone (5 x 10⁻⁷ M) or 19-norandrostenedione (5 x 10⁻⁷ M). In medium alone, 70.8% of oocytes spontaneously resumed meiosis as evidenced by the occurrence of germinal vesicle breakdown. Oocyte maturation was inhibited by (Bu)₂cAMP (44.6% of oocytes matured). Although neither steroid alone affected maturation, both testosterone and 19-norandrostenedione enhanced the effect of (Bu)₂cAMP (22.5 and 19.6%, respectively, resumed meiosis).

The effects of testosterone and 19-norandrostenedione on (Bu)₂cAMP-inhibited oocyte maturation were dose dependent and there was no significant difference between the actions of the steroids. The effect of 19-norandrostenedione was reversible and dependent on the presence of an intact cumulus. Hydroxyflutamide (SCH-16423), a nonsteroidal compound known to block androgen receptors, abolished the effects of both testosterone and 19-norandrostenedione on germinal vesicle breakdown, indicating that the actions of these steroids are truly androgenic.

The results of this study suggest that 19-norandrostenedione may be of physiological importance in the regulation of porcine oocyte maturation.

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Volume13, Issue2

February 1986

Pages 173-184

19-Norandrostenedione (4-estrene-3,17-dione) Inhibits Porcine Oocyte Maturation In Vitro

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19-Norandrostenedione (4-estrene-3,17-dione) Inhibits Porcine Oocyte Maturation In Vitro

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