RFC5, regulated by circ_0038985/miR-3614-5p, functions as an oncogene in the progression of colorectal cancer
Huihui Yao
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXin Zhou
Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorAina Zhou
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorJunjie Chen
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuoliang Chen
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXinyu Shi
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorBo Shi
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorQingliang Tai
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXiuwei Mi
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuoqiang Zhou
Department of Gastrointestinal Surgery, Changshu No. 2 Hospital, Suzhou, Jiangsu, China
Search for more papers by this authorSuo Wang
Department of Gastrointestinal Surgery, Changshu No. 1 Hospital, Suzhou, Jiangsu, China
Search for more papers by this authorJinbing Sun
Department of Gastrointestinal Surgery, Changshu No. 1 Hospital, Suzhou, Jiangsu, China
Search for more papers by this authorXiaodong Yang
Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorYi Yang
Department of Oncological Surgery, Kunshan Traditional Hospital Affiliated to Nanjing University of Chinese Medicine, Kunshan, Jiangsu, China
Search for more papers by this authorHuihua Cao
Department of Oncological Surgery, Kunshan Traditional Hospital Affiliated to Nanjing University of Chinese Medicine, Kunshan, Jiangsu, China
Search for more papers by this authorDiyuan Zhou
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorLiang Sun
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorYizhou Yao
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorCorresponding Author
Songbing He
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Correspondence Songbing He, Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.
Email: [email protected]
Search for more papers by this authorHuihui Yao
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXin Zhou
Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorAina Zhou
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorJunjie Chen
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuoliang Chen
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXinyu Shi
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorBo Shi
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorQingliang Tai
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXiuwei Mi
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuoqiang Zhou
Department of Gastrointestinal Surgery, Changshu No. 2 Hospital, Suzhou, Jiangsu, China
Search for more papers by this authorSuo Wang
Department of Gastrointestinal Surgery, Changshu No. 1 Hospital, Suzhou, Jiangsu, China
Search for more papers by this authorJinbing Sun
Department of Gastrointestinal Surgery, Changshu No. 1 Hospital, Suzhou, Jiangsu, China
Search for more papers by this authorXiaodong Yang
Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorYi Yang
Department of Oncological Surgery, Kunshan Traditional Hospital Affiliated to Nanjing University of Chinese Medicine, Kunshan, Jiangsu, China
Search for more papers by this authorHuihua Cao
Department of Oncological Surgery, Kunshan Traditional Hospital Affiliated to Nanjing University of Chinese Medicine, Kunshan, Jiangsu, China
Search for more papers by this authorDiyuan Zhou
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorLiang Sun
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorYizhou Yao
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorCorresponding Author
Songbing He
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Correspondence Songbing He, Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.
Email: [email protected]
Search for more papers by this authorHuihui Yao and Xin Zhou contributed equally to this work.
Abstract
Replication factor C 5 (RFC5) is involved in a variety of biological functions of cancer. However, the expression pattern of RFC5 and the underlying mechanisms in colorectal cancer (CRC) remain elusive. Here, we show that RFC5 is significantly upregulated in CRC tissues and cells. Patients with CRC and increased RFC5 levels have an unfavorable prognosis. RFC5 can promote the proliferation, migration, and invasion of CRC cells and inhibit the apoptosis of CRC cells. Additionally, upstream of RFC5, we constructed the competing endogenous RNA network and confirmed that RFC5 in this network was inhibited by miR-3614-5p by directly targeting its 3′-untranslated regions. We verified that circ_0038985, which is positively correlated with RFC5, directly targeted miR-3614-5p. Overexpression of circ_0038985 promoted CRC cell migration and invasion, and these effects were partially reversed by the reintroduction of miR-3614-5p. Moreover, we found that RFC5 may promote the vascular endothelial growth factor A (VEGFa)/vascular endothelial growth factor receptor 2 (VEGFR2)/extracellular signal-regulated protein kinase (ERK) pathway. The knockdown of RFC5 reduced CRC tumorigenesis in vivo. Collectively, these data demonstrate that the circ_0038985/miR-3614-5p/RFC5 axis plays a critical role in the progression of CRC, and RFC5 may promote CRC progression by affecting the VEGFa/VEGFR2/ERK pathway.
CONFLICT OF INTEREST STATEMENT
The authors declare no conflicts of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request. The original contributions presented in the study are included in the article/supplementary material. Further inquiries can be directed to the corresponding authors.
Supporting Information
| Filename | Description |
|---|---|
| mc23523-sup-0001-Supplementary_materials.7z13.3 MB | Supplementary information. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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