MiR-15a-3p regulates ferroptosis via targeting glutathione peroxidase GPX4 in colorectal cancer
Liurong Liu
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Department of General Surgery, Yancheng Third People's Hospital, Yancheng, Jiangsu, China
Search for more papers by this authorHuihui Yao
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXin Zhou
Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorJunjie Chen
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuoliang Chen
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXinyu Shi
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuanting Wu
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuoqiang Zhou
Department of Gastrointestinal Surgery, Changshu No. 2 Hospital, Suzhou, Jiangsu, China
Search for more papers by this authorCorresponding Author
Songbing He
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Correspondence Songbing He, Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.
Email: [email protected].
Search for more papers by this authorLiurong Liu
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Department of General Surgery, Yancheng Third People's Hospital, Yancheng, Jiangsu, China
Search for more papers by this authorHuihui Yao
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXin Zhou
Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorJunjie Chen
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuoliang Chen
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorXinyu Shi
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuanting Wu
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Search for more papers by this authorGuoqiang Zhou
Department of Gastrointestinal Surgery, Changshu No. 2 Hospital, Suzhou, Jiangsu, China
Search for more papers by this authorCorresponding Author
Songbing He
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Correspondence Songbing He, Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.
Email: [email protected].
Search for more papers by this authorLiurong Liu and Huihui Yao contributed equally to this work.
Abstract
Colorectal cancer (CRC) is the second most common cancer-related deaths throughout the world. Ferroptosis is a recently regulated form of cell death, lately gains attention. MicroRNA-15a-3p (miR-15a-3p) plays a regulatory role in various kinds of cancers. However, the role of miR-15a-3p in cellular ferroptosis is still unclear. Here, we aimed to clarify whether miR-15a-3p could regulate the ferroptosis of CRC. Here we identified miR-15a-3p positively regulates ferroptosis via directly targeting glutathione peroxidase glutathione peroxidase 4 (GPX4) in CRC. Overexpression of miR-15a-3p repressed GPX4 through binding to the 3′-untranslated region of GPX4, resulting in increased reactive oxygen species level, intracellular Fe2+ level, and malondialdehyde accumulation in vitro and in vivo. Correspondingly, suppression of miR-15a-3p reduced the sensitivity of CRC cells to erastin and GPX4. Taken together, these data demonstrate that miR-15a-3p regulates ferroptosis through targeting GPX4 in CRC cells, illustrating the novel role of microRNA in ferroptosis.
CONFLICT OF INTERESTS
The authors declare that there are no conflict of interests.
Open Research
DATA AVAILABILITY STATEMENT
The data used in the current study are available from the corresponding author on reasonable request.
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