Fabrication and Characterization of Reconstituted Silk Microgels for the Storage and Release of Small Molecules
Xizhou Liu
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorZenon Toprakcioglu
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorAlexander J. Dear
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorAviad Levin
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorFrancesco Simone Ruggeri
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorChristopher G. Taylor
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorMengsha Hu
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorJanet R. Kumita
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorMaria Andreasen
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Dr. M. Andreasen, Aarhus University, Wilhelm Meyer's Allé 3, 8000 Aarhus, Denmark
Search for more papers by this authorChristopher M. Dobson
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorUlyana Shimanovich
Dr. U. Shimanovich, Weizmann Institute of Science, Rehovot, 7600 Israel
Search for more papers by this authorCorresponding Author
Tuomas P. J. Knowles
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Prof. T. P. J. Knowles, Department of Physics, J J Thomson Avenue, Cambridge, CB3 0HE UK
E-mail: [email protected]Search for more papers by this authorXizhou Liu
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorZenon Toprakcioglu
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorAlexander J. Dear
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorAviad Levin
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorFrancesco Simone Ruggeri
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorChristopher G. Taylor
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorMengsha Hu
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorJanet R. Kumita
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorMaria Andreasen
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Dr. M. Andreasen, Aarhus University, Wilhelm Meyer's Allé 3, 8000 Aarhus, Denmark
Search for more papers by this authorChristopher M. Dobson
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Search for more papers by this authorUlyana Shimanovich
Dr. U. Shimanovich, Weizmann Institute of Science, Rehovot, 7600 Israel
Search for more papers by this authorCorresponding Author
Tuomas P. J. Knowles
X. Liu, Z. Toprakcioglu, A. J. Dear, Dr. A. Levin, Dr. F. S. Ruggeri, C. G. Taylor, M. Hu, Dr. J. R. Kumita, Dr. M. Andreasen, Prof. C. M. Dobson, Prof. T. P. J. Knowles, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW UK
Prof. T. P. J. Knowles, Department of Physics, J J Thomson Avenue, Cambridge, CB3 0HE UK
E-mail: [email protected]Search for more papers by this authorAbstract
Silk fibroin is a natural protein obtained from the Bombyx mori silkworm. In addition to being the key structural component in silkworm cocoons, it also has the propensity to self-assemble in vitro into hierarchical structures with desirable properties such as high levels of mechanical strength and robustness. Furthermore, it is an appealing biopolymer due to its biocompatability, low immunogenicity, and lack of toxicity, making it a prime candidate for biomedical material applications. Here, it is demonstrated that nanofibrils formed by reconstituted silk fibroin can be engineered into supramolecular microgels using a soft lithography-based microfluidic approach. Building on these results, a potential application for these protein microgels to encapsulate and release small molecules in a controlled manner is illustrated. Taken together, these results suggest that the tailored self-assembly of biocompatible and biodegradable silk nanofibrils can be used to generate functional micromaterials for a range of potential applications in the biomedical and pharmaceutical fields.
Conflict of Interest
The authors declare no conflict of interest.
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