Detection of sentinel nodes by a novel red-fluorescent dye, ATX-S10Na (II), in an orthotopic xenograft rat model of human gastric carcinoma
Tomoki Koyama MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorCorresponding Author
Akihito Tsubota MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan.Search for more papers by this authorKoichi Nariai PhD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorTetsuya Yoshikawa MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorMakoto Mitsunaga MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorMakoto Sumi MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorHiroshi Nimura MD
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorKatsuhiko Yanaga MD
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorYoko Yumoto BS
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorYasuo Mabashi BS
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorHiroshi Takahashi MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorTomoki Koyama MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorCorresponding Author
Akihito Tsubota MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan.Search for more papers by this authorKoichi Nariai PhD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorTetsuya Yoshikawa MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorMakoto Mitsunaga MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorMakoto Sumi MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorHiroshi Nimura MD
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorKatsuhiko Yanaga MD
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo 105-8461, Japan
Search for more papers by this authorYoko Yumoto BS
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorYasuo Mabashi BS
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorHiroshi Takahashi MD
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan
Search for more papers by this authorAbstract
Background and Objective
We developed a new imaging system to detect sentinel nodes (SNs) using a novel fluorescent tracer, ATX-S10Na(II), and investigated its usefulness in an animal model.
Study Design/Materials and Methods
Human gastric carcinoma cells were implanted orthotopically into nude rats. ATX-S10Na(II) was injected subserosally into the primary tumor lesion, and visualized by a fluorescence spectro-laparoscope. Presence of tumor cells in lymph nodes (LNs) was determined by RT-PCR specific for human β-actin.
Results
Injection of ATX-S10Na(II) was successful in 27 tumor-bearing rats. A red fluorescence was incorporated into the left gastric and hepatic LNs in 25 and 2 rats, respectively. Of note, human β-actin was detected in most of these LNs. Fluorescence was not detected in LNs that did not contain cancer.
Conclusion
ATX-S10Na(II) is useful for the detection of cancer-containing SNs in an animal model of gastric carcinoma, and may serve as a novel tracer in SN navigation surgery. Lasers Surg. Med. © 2006 Wiley-Liss, Inc.
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