Epithelial Tight Junction Anomalies in Nasal Inverted Papilloma
Zhi-Qun Huang PhD
Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, China
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Search for more papers by this authorXiang-Min Zhou MD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Department of Otolaryngology, Second Hospital of Shandong University, Jinan, China
Search for more papers by this authorTian Yuan PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Department of Otolaryngology-Head and Neck Surgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Search for more papers by this authorJing Liu PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Search for more papers by this authorHsiao H. Ong PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Search for more papers by this authorLi-Ying Sun PhD
First School of Clinical Medicine, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorJun-Hao Tu PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China
Search for more papers by this authorMeng-Yue Li MD
Department of Otorhinolaryngology, Changji Branch, The First Affiliated Hospital of Xinjiang Medical University, Changji, China
Search for more papers by this authorKim T. M. Thong MD
Department of Otolaryngology, Head and Neck Surgery, National University Health System, Singapore, Singapore
Search for more papers by this authorJing Ye PhD
Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China
Search for more papers by this authorLi Shi PhD
Department of Otolaryngology, Second Hospital of Shandong University, Jinan, China
Search for more papers by this authorCorresponding Author
De-Yun Wang MD, PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Send correspondence to Yu Xu, MD, PhD, Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, China. Email: [email protected]
De-Yun Wang, MD, PhD, Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Rd, Singapore 119228, Singapore. Email: [email protected]
Search for more papers by this authorCorresponding Author
Yu Xu MD, PhD
Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, China
Send correspondence to Yu Xu, MD, PhD, Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, China. Email: [email protected]
De-Yun Wang, MD, PhD, Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Rd, Singapore 119228, Singapore. Email: [email protected]
Search for more papers by this authorZhi-Qun Huang PhD
Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, China
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Search for more papers by this authorXiang-Min Zhou MD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Department of Otolaryngology, Second Hospital of Shandong University, Jinan, China
Search for more papers by this authorTian Yuan PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Department of Otolaryngology-Head and Neck Surgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Search for more papers by this authorJing Liu PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Search for more papers by this authorHsiao H. Ong PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Search for more papers by this authorLi-Ying Sun PhD
First School of Clinical Medicine, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorJun-Hao Tu PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China
Search for more papers by this authorMeng-Yue Li MD
Department of Otorhinolaryngology, Changji Branch, The First Affiliated Hospital of Xinjiang Medical University, Changji, China
Search for more papers by this authorKim T. M. Thong MD
Department of Otolaryngology, Head and Neck Surgery, National University Health System, Singapore, Singapore
Search for more papers by this authorJing Ye PhD
Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China
Search for more papers by this authorLi Shi PhD
Department of Otolaryngology, Second Hospital of Shandong University, Jinan, China
Search for more papers by this authorCorresponding Author
De-Yun Wang MD, PhD
Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore
Send correspondence to Yu Xu, MD, PhD, Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, China. Email: [email protected]
De-Yun Wang, MD, PhD, Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Rd, Singapore 119228, Singapore. Email: [email protected]
Search for more papers by this authorCorresponding Author
Yu Xu MD, PhD
Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, China
Send correspondence to Yu Xu, MD, PhD, Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, China. Email: [email protected]
De-Yun Wang, MD, PhD, Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Rd, Singapore 119228, Singapore. Email: [email protected]
Search for more papers by this authorThis work was supported by the China Scholarship Council (No. 202206270150); the National Natural Science Foundation of China (No. 81770986 and 82071017); the Central University Basic Research Fund of China (No. 2042022kf1113); the National Medical Research Council (NMRC/CIRG/1458/2016), Singapore; and the Science and Technology Project of Jiangxi Provincial Health Commission (No. 202110119), China.
The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Abstract
Objectives
As a critical component of the epithelial barrier, tight junctions (TJs) are essential in nasal mucosa against pathogen invasion. However, the function of TJs has rarely been reported in nasal inverted papilloma (NIP). This study aims to investigate the potential factors of TJs' abnormality in NIP.
Methods
We assessed the expression of ZO-1, occludin, claudin-1, claudin-3, and claudin-7 in healthy controls and NIP by real-time quantitative polymerase chain reaction and immunofluorescent staining. The correlation between TJs expression and neutrophil count, TH1/TH2/TH17 and regulatory T cell biomarkers, and the proportion of nasal epithelial cells was investigated.
Results
Upregulation of ZO-1, occludin, claudin-1, and claudin-7, along with downregulation of claudin-3, was found in NIP compared to control (all p < 0.05). An abnormal proportion with a lower number of ciliated cells (control vs. NIP: 37.60 vs. 8.67) and goblet cells (12.52 vs. 0.33) together with a higher number of basal cells (45.58 vs. 124.00) in NIP. Meanwhile, claudin-3 was positively correlated with ciliated and goblet cells (all p < 0.01). Additionally, neutrophils were excessively infiltrated in NIP, negatively correlated with ZO-1, but positively with claudin-3 (all p < 0.05). Furthermore, FOXP3, IL-10, TGF-β1, IL-5, IL-13, and IL-22 levels were induced in NIP (all p < 0.01). Occludin level was negatively correlated with IL-10, IL-5, IL-13, and IL-22, whereas ZO-1 was positively with TGF-β1 (all p < 0.05).
Conclusion
Nasal epithelial barrier dysfunction with TJs anomalies is commonly associated with abnormal proliferation and differentiation of epithelial cells and imbalance of immune and inflammatory patterns in NIP.
Level of Evidence
NA Laryngoscope, 134:552–561, 2024
Supporting Information
| Filename | Description |
|---|---|
| lary30845-sup-0001-supinfo.docxWord 2007 document , 15.5 MB | Appendix S1. Supporting information. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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