Volume 128, Issue 10 pp. E339-E345

Allergy/Rhinology

In Vivo Biofilm Formation, Gram-Negative Infections and TAS2R38 Polymorphisms in CRSw NP Patients

Elena Cantone MD, PhD,

Elena Cantone MD, PhD

Department of Neuroscience, ENT Section

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Rossella Negri PhD,

Corresponding Author

Rossella Negri PhD

[email protected]

orcid.org/0000-0002-7372-681X

Department of Translational Medical Science (DISMET), Section of Pediatrics

Send correspondence to Rossella Negri, MD, Department of Translational Medical Science (DISMET), Section of Pediatrics, University of Naples “Federico II” via Pansini 5, 80131, Naples, Italy. E-mail: [email protected]Search for more papers by this author

Emanuela Roscetto PhD,

Emanuela Roscetto PhD

Department of Molecular Medicine and Medical Biotechnology, Section of Clinical Microbiology, University of Naples “Federico II”

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Rossella Grassia MD,

Rossella Grassia MD

Department of Otolaryngology Head–Neck Surgery, Monaldi-Ospedale dei Colli, Naples, Italy.

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Maria Rosaria Catania PhD,

Maria Rosaria Catania PhD

Department of Molecular Medicine and Medical Biotechnology, Section of Clinical Microbiology, University of Naples “Federico II”

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Pasquale Capasso MD,

Pasquale Capasso MD

Department of Otolaryngology Head–Neck Surgery, Monaldi-Ospedale dei Colli, Naples, Italy.

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Marianna Maffei MD,

Marianna Maffei MD

Department of Otolaryngology Head–Neck Surgery, Monaldi-Ospedale dei Colli, Naples, Italy.

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Amata Amy Soriano MD,

Amata Amy Soriano MD

Department of Molecular Medicine and Medical Biotechnology, Section of Clinical Microbiology, University of Naples “Federico II”

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Carlo Antonio Leone MD,

Carlo Antonio Leone MD

Department of Otolaryngology Head–Neck Surgery, Monaldi-Ospedale dei Colli, Naples, Italy.

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Maurizio Iengo MD,

Maurizio Iengo MD

Department of Neuroscience, ENT Section

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Luigi Greco MD, PhD,

Luigi Greco MD, PhD

Department of Translational Medical Science (DISMET), Section of Pediatrics

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Elena Cantone MD, PhD,

Elena Cantone MD, PhD

Department of Neuroscience, ENT Section

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Rossella Negri PhD,

Corresponding Author

Rossella Negri PhD

[email protected]

orcid.org/0000-0002-7372-681X

Department of Translational Medical Science (DISMET), Section of Pediatrics

Emanuela Roscetto PhD,

Emanuela Roscetto PhD

Department of Molecular Medicine and Medical Biotechnology, Section of Clinical Microbiology, University of Naples “Federico II”

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Rossella Grassia MD,

Rossella Grassia MD

Department of Otolaryngology Head–Neck Surgery, Monaldi-Ospedale dei Colli, Naples, Italy.

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Maria Rosaria Catania PhD,

Maria Rosaria Catania PhD

Department of Molecular Medicine and Medical Biotechnology, Section of Clinical Microbiology, University of Naples “Federico II”

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Pasquale Capasso MD,

Pasquale Capasso MD

Department of Otolaryngology Head–Neck Surgery, Monaldi-Ospedale dei Colli, Naples, Italy.

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Marianna Maffei MD,

Marianna Maffei MD

Department of Otolaryngology Head–Neck Surgery, Monaldi-Ospedale dei Colli, Naples, Italy.

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Amata Amy Soriano MD,

Amata Amy Soriano MD

Department of Molecular Medicine and Medical Biotechnology, Section of Clinical Microbiology, University of Naples “Federico II”

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Carlo Antonio Leone MD,

Carlo Antonio Leone MD

Department of Otolaryngology Head–Neck Surgery, Monaldi-Ospedale dei Colli, Naples, Italy.

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Maurizio Iengo MD,

Maurizio Iengo MD

Department of Neuroscience, ENT Section

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Luigi Greco MD, PhD,

Luigi Greco MD, PhD

Department of Translational Medical Science (DISMET), Section of Pediatrics

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First published: 23 March 2018

https://doi.org/10.1002/lary.27175

Citations: 33

The authors have no funding, financial relationships, or conflicts of interest to disclose.

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Abstract

Objectives

Among the predisposing factors implicated in the immune response to airway bacterial infections, genetic variations of the bitter taste receptor TAS2R38, which is expressed in the cilia of the human sinonasal epithelial cells, seem to be associated with susceptibility to chronic rhinosinusitis (CRS) and in vitro biofilm formation. Polymorphisms in TAS2R38 generate two common haplotypes: the nonfunctional AVI (Alanine, Valine, Isoleucine) and the functional PAV (Proline, Alanine, Valine) alleles, with the latter protecting against gram-negative sinonasal infections.

The aim of this study is to investigate for the first time the relevance of TAS2R38 genetic variants in the susceptibility to bacterial infections associated with in vivo biofilm formation in chronic rhinosinusitis with nasal polyps (CRSwNP) patients.

Study Design

A prospective study on 100 adult patients undergoing functional endoscopic sinus surgery (FESS) for CRSwNP.

Methods

Propylthiouracile (PROP) testing and TAS2R38 genotyping were applied to characterize patients for receptor functionality. Sinonasal mucosa samples were processed for microbiological examination and biofilm detection.

Results

The nonfunctional genotype is more frequent among CRS patients than in the general population (25% vs. 18.4%, P = 0.034). Airway gram-negative infections are primarily associated with the AVI haplotype (88.9% vs. 11.1% PAV/PAV-functional genotype, P = 0.023). Biofilm formation is prevalent in CRS patients with the AVI nontaster phenotype (62.5% vs. 33.3% PAV taster or supertaster phenotype, P = 0.05).

Conclusion

Our findings confirm an inverse correlation between TAS2R38 functionality and gram-negative infections in Italian patients with CRSwNP. In addition, for the first time we demonstrated a relationship between in vivo microbial biofilm and TAS2R38 receptor variants.

Level of Evidence

2b. Laryngoscope, 128:E339–E345, 2018

Supporting Information

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Citing Literature

Volume128, Issue10

October 2018

Pages E339-E345

Filename	Description
lary27175-sup-0001-suppinfo1.docx35.4 KB	SUPPORTING FIG. S1. Correlation between perceived intensity of 0.28 mM PROP, the lowest concentration able to discriminate Tasters from Non-tasters, and the TAS2R38 genotype. (ANOVA F=8.621; p=0.000).
lary27175-sup-0002-suppinfo2.tif111.9 KB	SUPPORTING FIG. S2. Lack of correlation between the clinical metrics (LK, LM, SNOT-22) and PROP sensitivity (p>0.05, Pearson correlation).
lary27175-sup-0003-suppinfo3.docx18.8 KB	SUPPORTING Table SI. Correlation between the TAS2R38 genotype and detection of bacterial biofilm on the nasal mucosa of RSC patients.

In Vivo Biofilm Formation, Gram-Negative Infections and TAS2R38 Polymorphisms in CRSw NP Patients

Abstract

Objectives

Study Design

Methods

Results

Conclusion

Level of Evidence

Supporting Information

BIBLIOGRAPHY

Citing Literature

References

Information

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In Vivo Biofilm Formation, Gram-Negative Infections and TAS2R38 Polymorphisms in CRSw NP Patients

Abstract

Objectives

Study Design

Methods

Results

Conclusion

Level of Evidence

Supporting Information

BIBLIOGRAPHY

Citing Literature

References

Related

Information