Effect of radiation and cell implantation on wound healing in a rat model
Dale Dantzer MD
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorPeter Ferguson MD, FRCSC
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorRichard P. Hill PhD
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
Search for more papers by this authorArmand Keating MD, FRCPC
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
Search for more papers by this authorRita A. Kandel MD, FRCPC
Department of Pathology, Mount Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorJay S. Wunder MD, FRCSC
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorBrian O'Sullivan MD, FRCPC
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
Search for more papers by this authorJas Sandhu PhD
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorJennifer Waddell MSc
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorCorresponding Author
Robert S. Bell MD, FRCSC
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
Suite 476-E, Mount Sinai Hospital, Toronto, Ontario, Canada. M5G 1X5. Fax: 416-946-4585.Search for more papers by this authorDale Dantzer MD
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorPeter Ferguson MD, FRCSC
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorRichard P. Hill PhD
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
Search for more papers by this authorArmand Keating MD, FRCPC
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
Search for more papers by this authorRita A. Kandel MD, FRCPC
Department of Pathology, Mount Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorJay S. Wunder MD, FRCSC
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorBrian O'Sullivan MD, FRCPC
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
Search for more papers by this authorJas Sandhu PhD
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorJennifer Waddell MSc
The Samuel Lunenfeld Research Institute, Sinai Hospital, Toronto, Ontario, Canada
Search for more papers by this authorCorresponding Author
Robert S. Bell MD, FRCSC
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada
Suite 476-E, Mount Sinai Hospital, Toronto, Ontario, Canada. M5G 1X5. Fax: 416-946-4585.Search for more papers by this authorAbstract
Background and Objectives
Having shown that intra-dermal injection of fibroblasts decreases the effect of radiation on healing of superficial wounds, we now test the effect of fibroblasts and syngeneic marrow stromal cells on irradiated deep and superficial wounds.
Methods
Wistar rats received bilateral buttock irradiation followed by partial excision of the gluteus muscle bilaterally. In Protocol 1, one irradiated wound was treated with 1.2 × 107 autologous cells injected intra-dermally. In Protocol 2, the experimental side was treated with a fibrin and autologous cell implant (1.2 × 107 cells). Twenty-one days later, wound mechanical characteristics were tested. In Protocol 3, the effect of pooled marrow stromal cells on healing of superficial irradiated wounds in Lewis rats was similarly tested.
Results
The fibrin–fibroblast implant (Protocol 2) had no effect on wound mechanics. Superficial injection of fibroblasts (Protocol 1) significantly improved wound breaking strength when compared to the control group (mean ± SEM, breaking strength: treated 504.6 ± 37.0 g vs. control 353.4 ± 35.2 g, P = 0.005). The dermal injection of marrow stromal cells also resulted in marked increases in breaking strength (mean ± SEM, breaking strength: treated 338.5 ± 39.9 g vs. control 187.1 ± 12.0 g, P < 0.01). In both Protocols 1 and 3, ultimate tensile strength and toughness were increased in the side receiving cell transplantation.
Conclusions
Cell implantation holds promise for decreasing the effect of radiation on healing of irradiated wounds. J. Surg. Oncol. 2003;83:185–190. © 2003 Wiley-Liss, Inc.
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