Phenotype and chondrogenic differentiation of mesenchymal cells from adipose tissue of different species
Corresponding Author
María José Martínez-Lorenzo
Banco de Sangre y Tejidos de Aragón, Área de Tejidos, Zaragoza, Spain
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Banco de Sangre y Tejidos de Aragón, Área de Tejidos, Zaragoza, Spain, T: 34-876 764-300; F: 34-876-764-321.Search for more papers by this authorMaría Royo-Cañas
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Search for more papers by this authorElena Alegre-Aguarón
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Servicio de Inmunología del Hospital Clínico Universitario Lozano Blesa
Search for more papers by this authorPaula Desportes
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Servicio de Inmunología del Hospital Clínico Universitario Lozano Blesa
Search for more papers by this authorTomás Castiella
Servicio de Anatomía Patológica del Hospital Clínico Universitario Lozano Blesa
Search for more papers by this authorFelícito García-Álvarez
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Servicio de Traumatología del Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain
Search for more papers by this authorLuis Larrad
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Servicio de Inmunología del Hospital Clínico Universitario Lozano Blesa
Search for more papers by this authorCorresponding Author
María José Martínez-Lorenzo
Banco de Sangre y Tejidos de Aragón, Área de Tejidos, Zaragoza, Spain
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Banco de Sangre y Tejidos de Aragón, Área de Tejidos, Zaragoza, Spain, T: 34-876 764-300; F: 34-876-764-321.Search for more papers by this authorMaría Royo-Cañas
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Search for more papers by this authorElena Alegre-Aguarón
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Servicio de Inmunología del Hospital Clínico Universitario Lozano Blesa
Search for more papers by this authorPaula Desportes
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Servicio de Inmunología del Hospital Clínico Universitario Lozano Blesa
Search for more papers by this authorTomás Castiella
Servicio de Anatomía Patológica del Hospital Clínico Universitario Lozano Blesa
Search for more papers by this authorFelícito García-Álvarez
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Servicio de Traumatología del Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain
Search for more papers by this authorLuis Larrad
Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain
Servicio de Inmunología del Hospital Clínico Universitario Lozano Blesa
Search for more papers by this authorAbstract
Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into several mesoderm lineages. They have been isolated from different tissues, such as bone marrow, adult peripheral blood, umbilical cord blood, and adipose tissue. The aim of this study was to analyze the differences in proliferation and phenotype of adipose tissue-derived MSCs from three different species, and to evaluate their capacity to differentiate into chondrocytes in vitro. A comparative study of cultured human, rabbit, and sheep mesenchymal cells from adipose tissue was carried out, and the main morphological parameters, proliferative activity, and expression of surface markers were characterized. Proliferation and flow cytometry data showed species-related differences between animal and human MSCs. Histological staining suggested that rabbit and sheep mesenchymal cells were able to differentiate into chondrocytic lineages. Human mesenchymal cells, though they could also differentiate, accomplished it with more difficulty than animal MSCs. These results could help to explain the differences in the chondrogenic capacity of sheep and rabbit MSCs when they are used as animal models compared to human mesenchymal cells in a clinical assay. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1499–1507, 2009
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