Article
Full Access
Effect of direct current on cultured growth cartilage cells in vitro
Dr. Hiroyuki Okihana,
Yutaka Shimomura,
Corresponding Author
Dr. Hiroyuki Okihana
Department of Orthopaedic Surgery, National Defencse Medical College, Namiki, Tokorozawa-shi, Saitama Prefecture, Japan
Department of Orthopaedic Surgery, National Defense Medical College, 3-2. Namiki, Tokorozawa-shi, Saitama Prefecture 359, JapanSearch for more papers by this authorYutaka Shimomura
Department of Orthopaedic Surgery, National Defencse Medical College, Namiki, Tokorozawa-shi, Saitama Prefecture, Japan
Search for more papers by this authorDr. Hiroyuki Okihana,
Yutaka Shimomura,
Corresponding Author
Dr. Hiroyuki Okihana
Department of Orthopaedic Surgery, National Defencse Medical College, Namiki, Tokorozawa-shi, Saitama Prefecture, Japan
Department of Orthopaedic Surgery, National Defense Medical College, 3-2. Namiki, Tokorozawa-shi, Saitama Prefecture 359, JapanSearch for more papers by this authorYutaka Shimomura
Department of Orthopaedic Surgery, National Defencse Medical College, Namiki, Tokorozawa-shi, Saitama Prefecture, Japan
Search for more papers by this authorAbstract
Cultured growth cartilage cells of young rabbits were stimulated by three levels(10, l and 0.l μA) of constant direct current (DC). The effect of DC was examined by measuring the activities of proteoglycan synthesis (uptake of [35S]sodium sulfate and toluidine blue staining) and DNA synthesis (uptake of [3H]thymidine). DC increased syntheses of both proteoglycans and DNA when l μA calculated curren densiy of l μA/cm2 was applied for 3 days. A longer stimulation resulted in a larger difference between stimulated and unstimulted cells. Ten-microampere stimulation devitalized the cells, while 0.l μA had no effect on cell activities.
References
- 1 Baranowski TJ, Black J, Brighton CT, Friedenberg ZB: Electrical osteogenesis by low direct current. J Orthop Res 1: 120–128, 1983
- 2 Bassett CAL, Becker RO: Generation of electric potentials in bone in response to mechanical stress. Science 137: 1063–1064, 1962
- 3 Bassett CAL, Mitchell SN, Norton L, Caulo N, Gaston SR: Electromagnetic reparis of nonunions. In: Electrical Properties of Bone CArtilage, ed by CT Brighton et al., New York, Grune & Stratton, 1979, pp 605–630
- 4 Bassett CAL, Mitchell SN, Gsston SR: Treatment of ununited tibial diaphyseal fractures with pulsing electromagnetic fields. J Bone Joint Surg[Am] 63: 511–523, 1981
- 5 Becker RO: The bioelectric factors in amphibian limb regeneration. J Bone Joint Surg [Am] 43: 643–656, 1961
- 6 Becker RO, Murray DG: The electrical control system regulating fracture healing in amphibians. Clin Orthop 73: 169–198, 1970
- 7 Brighton CT: A multicenter study of the treatment of nonunion with constant direct current. J Bone Joint Surg [A] 63: 2–13, 1981
- 8 Brighton CT: Treatmen of nonunion of the tibia with constant direct current. J Trauma 21: 189–195, 1981
- 9 Brighton CT, Cronkey JE, Osterman AL: In vitro epiphyseal plate growth in various constant electrical fields. J Bone Joint Surg [AM] 58: 971–978, 1976
- 10 Brighton CT, Friedenberg ZB, Mitchell EL, Booth RE: Treatment of nonunion with constant direct current. Clin Orthop 124: 106–123, 1977
- 11 Brighton CT, Pfeffer GB, Pollack SR: In vivo growth plate stimulation in various capacitively coupled electrical fields. J Orthop Res 1: 42–49, 1983
- 12 Brighton CT, Ungar AS, Stambough JL: In vivo growth of bovine articular cartilage chondrocytes in various capacitive coupled electrical fileds. J Orthop Res 2: 15–22, 1984
- 13 Friedenberg ZB, Brighton CT: Bioelectric potentials in bone. J Bone Joint Surg [Am] 48: 915–923, 1966
- 14 Freidenberg ZB, Zemsky LM, Pollis RP, Brighton CT: The response of non-traumatized bone to direct current. J Bone Joint Surg [Am] 56; 1023–1030, 1974
- 15 Fukada E, Yasuda I: On the piezoelectric effect of bone. J Phys Soc Jpn 12: 1158–1162, 1957
- 16 Fukada E, Yasuda I: Piezoelectric effects in collagen. Jpn J Appl Phys 3: 117–121, 1964
- 17 Harris WH, Moyen BJL, Thrashe EL, et al.: Differential response to electrical stimulaion. Clin orthop 124: 31–40, 1977
- 18 Iida H: Study on dynamic and electric calluses of bone in vitro [Japanese]. J Jpn Orthop Assoc 31: 643–664, 1957
- 19 Iida H, Shuntan K, Miyashita Y, et al.: Electric callus produced by an alternating current [Japanese]. J Kyoto Pref Med Univ 60: 561–564, 1956
- 20 Inoue S, Ohashi T, Lmai R, Ichida M, Yasuda I: The electrical induction of callus formation and external skeletal fixation using methyl methacrylate for delayed union of open tibial fracture with segmental loss. Clin Orthop 124: 92–96, 1977
- 21 Inoue S, Ohashi T, Kajikawa K, Ibaraki K, Sakaki H, Tada M: Application of electric stimulation methods for bone fracture and disease [Japanese]. Clin Orthop Surg 18: 1291–1298, 1983
- 22 Küntscher G: Callus ohne Knochenbrush. Zentrabl Chir 68: 857–868, 1941
- 23 Maatz R: Die Reaktion des Knochen auf Federdruck. Arch orthop Unfall-Chir 44: 529–539, 1951
- 24 Norton LA: Effects of a pulsed electromagnetic field on a mixed chondroblastic tissue culture. Clin Orthop 167: 280–290, 1982
- 25 Paterson DC, Hillier TM, Carter RF, Ludbrook J, Maxwell GM, Savage JP: Experimental delayed union of the dog tibia and its use in assessing the effet of an electrical bone growth stimulator. Clin Orthop 128: 340–350, 1977
- 26 Shamos M, Lavine LS, Shamos Ml: Piezoelectric effect in bone. Nature 197: 81, 1963
- 27 Shimomura Y, Suzuki F: Cultured growth cartilage cells. Clin Orthop 184: 93–105, 1984
- 28 Suzuki Y: Clinical application of callus formation by electrical stimulation and electromagnetic fields [Japanese]. Orthop Trauma Surg 25: 1097–1108, 1982
- 29 Takahashi H, Togawa Y, Konno T, Suzuki H, Shinokura H, Abe HL: Effect of mcrocurrent on bone resorption [Japanese]. Orthop Res Sci 4: 145–154, 1977
- 30 Watson J, de Hass WG, Hauser SS: Effect of electric fields on growth rate of embryonic chick tibiae in vitro. Naute 25: 331–332, 1975
- 31 Yasuda I: Mechanical and electrical callus. Ann NY Acad Sci 238: 457–465, 1974
- 32 Yasuda I: Nagayama H, Kato T et al: Fundamental problems in the treatment of frature [Japanese]. J Kyoto Med Soc 4: 95–406, 1953
- 33 Yasuda I, Noguchi K, Iida H: Application of electrical callus [Japanese]. J Jpn Orthop Assoc 29: 351–353, 1955
- 34 Yukawa, Y, Mano J, Tsuda Y, et al.: Pathological character of the electrical callus by AC stimulation [Japanese]. Orthop Trauma Surg 25: 1079–1090, 1982
- 35 Yukawa Y, Tsuda Y, Furuya K, et al: Characteristics of the electrical callus by AC stimulation [Japanese]. Clin Orthop Surg 18: 1267–1280, 1983
- 36 Yukawa Y, Tsuda Y, Furuya K, et al.: On the electrodynamics of the electrical callus in time course, and the impedance value of bone and soft tissue. In: Bioelectrical Repair and Growth, ed by E F et al., Nishimura Niigata, 1985, pp 251–256
