First report on fatal myocarditis associated with adenovirus infection in Cuba
Corresponding Author
Odalys Valdés
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Instituto de Medicina Tropical Pedro Kourí, Ciudad de la Habana, Cuba; Autopista Novia del Mediodía km 6½, la Lisa, P.O. Box 601, Marianao 13, CP 17 100, Cuba.===Search for more papers by this authorBelsy Acosta
National Centre of Influenza, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorAlexander Piñón
National Centre of Influenza, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorClara Savón
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorAngel Goyenechea
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorGrehete Gonzalez
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorGuelsys Gonzalez
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorLidice Palerm
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorLuis Sarmiento
Enteroviruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorMas Lago Pedro
Enteroviruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorPedro A. Martínez
Sexually Transmitted Diseases Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorDelfina Rosario
Arboviruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorVivian Kourí
Sexually Transmitted Diseases Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorMaría Guadalupe Guzmán
Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorAlina Llop
Microbiology Division, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorInmaculada Casas
Virology Department, Respiratory Viruses Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
Search for more papers by this authorMa. Pilar Perez Breña
Virology Department, Respiratory Viruses Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
Search for more papers by this authorCorresponding Author
Odalys Valdés
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Instituto de Medicina Tropical Pedro Kourí, Ciudad de la Habana, Cuba; Autopista Novia del Mediodía km 6½, la Lisa, P.O. Box 601, Marianao 13, CP 17 100, Cuba.===Search for more papers by this authorBelsy Acosta
National Centre of Influenza, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorAlexander Piñón
National Centre of Influenza, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorClara Savón
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorAngel Goyenechea
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorGrehete Gonzalez
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorGuelsys Gonzalez
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorLidice Palerm
Respiratory Viruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorLuis Sarmiento
Enteroviruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorMas Lago Pedro
Enteroviruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorPedro A. Martínez
Sexually Transmitted Diseases Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorDelfina Rosario
Arboviruses Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorVivian Kourí
Sexually Transmitted Diseases Laboratory, Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorMaría Guadalupe Guzmán
Virology Department, Division of Microbiology, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorAlina Llop
Microbiology Division, Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba
Search for more papers by this authorInmaculada Casas
Virology Department, Respiratory Viruses Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
Search for more papers by this authorMa. Pilar Perez Breña
Virology Department, Respiratory Viruses Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
Search for more papers by this authorAbstract
Myocarditis is caused frequently by viral infections of the myocardium. In the past, enteroviruses (EV) were considered the most common cause of myocarditis in all age groups. Other viruses that cause myocarditis are adenovirus and influenza viruses. Parvovirus B19 infection is associated sometimes with myocarditis. Members of the Herpesviridae family, cytomegalovirus (CMV), and human herpesvirus 6 (HHV-6) have been associated occasionally with myocarditis. During an atypical outbreak of acute febrile syndrome, eight children, with ages from 5 months to 15 years, died in cardiogenic shock due to myocarditis in July–August 2005, in the city of Havana, Cuba. Nested polymerase chain reaction (nPCR) and nested reverse transcription-PCR (nRT-PCR) were carried out on fresh heart muscle and lung tissue to analyze the genomic sequences of adenovirus, CMV, HHV-6, herpes simplex virus, Epstein–Barr virus (EBV), varizella zoster virus, influenza virus A, B, C, respiratory syncytial virus (RSV) A and B, parainfluenza viruses, rhinoviruses, coronavirus, flaviruses and enteroviruses. Evidence was for the presence of the adenovirus genome in 6 (75%) of the children. Phylogenetic analyses of a conserved hexon gene fragment in four cases showed serotype 5 as the causal agent. No others viruses were detected. Histological examination was undertaken to detect myocardial inflammation. After exclusion of other possible causes of death, the results indicated that viral myocarditis was the cause of death in patients with adenovirus infection. J. Med. Virol. 80:1756–1761, 2008. © 2008 Wiley-Liss, Inc.
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