Very low density lipoprotein and low density lipoprotein isolated from patients with hepatitis C infection induce altered cellular lipid metabolism
Mariarosaria Napolitano
Department of Haematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy
Search for more papers by this authorAlessandro Giuliani
Department of Environment and Health, Istituto Superiore di Sanità, Rome, Italy
Search for more papers by this authorTonino Alonzi
National Institute for Infectious Diseases “Lazzaro Spallanzani” I.R.C.C.S., Rome, Italy
Search for more papers by this authorCarmine Mancone
National Institute for Infectious Diseases “Lazzaro Spallanzani” I.R.C.C.S., Rome, Italy
Search for more papers by this authorGianpiero D'Offizi
National Institute for Infectious Diseases “Lazzaro Spallanzani” I.R.C.C.S., Rome, Italy
Search for more papers by this authorMarco Tripodi
National Institute for Infectious Diseases “Lazzaro Spallanzani” I.R.C.C.S., Rome, Italy
Fondazione “Istituto Pasteur Cenci-Bolognetti”, Dipartimento di Biotecnologie Cellulari ed Ematologia, Università La Sapienza, Rome, Italy
Search for more papers by this authorCorresponding Author
Elena Bravo
Department of Haematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy
Department of Haematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome Italy.===Search for more papers by this authorMariarosaria Napolitano
Department of Haematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy
Search for more papers by this authorAlessandro Giuliani
Department of Environment and Health, Istituto Superiore di Sanità, Rome, Italy
Search for more papers by this authorTonino Alonzi
National Institute for Infectious Diseases “Lazzaro Spallanzani” I.R.C.C.S., Rome, Italy
Search for more papers by this authorCarmine Mancone
National Institute for Infectious Diseases “Lazzaro Spallanzani” I.R.C.C.S., Rome, Italy
Search for more papers by this authorGianpiero D'Offizi
National Institute for Infectious Diseases “Lazzaro Spallanzani” I.R.C.C.S., Rome, Italy
Search for more papers by this authorMarco Tripodi
National Institute for Infectious Diseases “Lazzaro Spallanzani” I.R.C.C.S., Rome, Italy
Fondazione “Istituto Pasteur Cenci-Bolognetti”, Dipartimento di Biotecnologie Cellulari ed Ematologia, Università La Sapienza, Rome, Italy
Search for more papers by this authorCorresponding Author
Elena Bravo
Department of Haematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy
Department of Haematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome Italy.===Search for more papers by this authorAbstract
Several abnormalities of lipid metabolism, including hypo-β-lipoproteinemia and liver steatosis are associated with infection by hepatitis C virus (HCV). The aim of this study was to determine whether circulating lipoproteins of patients with HCV infection could directly cause alterations of lipid cellular metabolism. To this end the metabolic response of human monocyte-derived macrophages (HMDM) to very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL), measuring the cholesteryl ester (CE) and triglyceride (TG) production was analyzed. Lipoproteins were isolated from 18 patients infected with hepatitis C virus (HCV-VLDL and HCV-LDL) and from normal healthy donors (ct-VLDL and ct-LDL). In comparison to ct-lipoproteins, HCV-lipoproteins induced significant differences in HMDM CE and TG production. HCV-VLDL decreased CE and TG production; while HCV-LDL induced an increased TG synthesis. The present findings suggest that HCV infection modifies VLDL and LDL molecular composition, affecting cellular lipid metabolism, thus promoting intracellular lipid accumulation and hypo-β-lipoproteinemia. J. Med. Virol. 79:254–258, 2007. © 2007 Wiley-Liss, Inc.
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