Rapid detection of virus genome from imported dengue fever and dengue hemorrhagie fever patients by direct polymerase chain reaction
Corresponding Author
Kouichi Morita M.D., Ph.D.
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
Department of Virology, Institute of Tropical Medicine, Nagasaki University, 12–4, Sakamoto Nagasaki, Japan 852===Search for more papers by this authorTatsuo Maemoto
Department of Pediatrics, Asahi-chuo Hospital, Chiba, Japan
Search for more papers by this authorShoji Honda
Department of Pediatrics, Asahi-chuo Hospital, Chiba, Japan
Search for more papers by this authorKenji Onishi
Department of Communicable Disease, Bokuto Metropolitan Hospital, Tokyo, Japan
Search for more papers by this authorMisako Murata
Department of Communicable Disease, Bokuto Metropolitan Hospital, Tokyo, Japan
Search for more papers by this authorMariko Tanaka
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
Search for more papers by this authorAkira Igarashi
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
Search for more papers by this authorCorresponding Author
Kouichi Morita M.D., Ph.D.
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
Department of Virology, Institute of Tropical Medicine, Nagasaki University, 12–4, Sakamoto Nagasaki, Japan 852===Search for more papers by this authorTatsuo Maemoto
Department of Pediatrics, Asahi-chuo Hospital, Chiba, Japan
Search for more papers by this authorShoji Honda
Department of Pediatrics, Asahi-chuo Hospital, Chiba, Japan
Search for more papers by this authorKenji Onishi
Department of Communicable Disease, Bokuto Metropolitan Hospital, Tokyo, Japan
Search for more papers by this authorMisako Murata
Department of Communicable Disease, Bokuto Metropolitan Hospital, Tokyo, Japan
Search for more papers by this authorMariko Tanaka
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
Search for more papers by this authorAkira Igarashi
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
Search for more papers by this authorAbstract
Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT-PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. The results of PCR corresponded closely to the results of virus isolation using cultured mosquito cells, suggesting that direct RT-PCR procedure greatly facilitates rapid diagnosis of dengue infection. © 1994 Wiley-Liss, Inc.
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