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Sequence variation within the human immunodeficiency virus V3 loop at seroconversion
Mounir Ait-Khaled,
Vincent C. Emery,
Corresponding Author
Mounir Ait-Khaled
Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill Street, London, United Kingdom
Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, United Kingdom===Search for more papers by this authorVincent C. Emery
Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill Street, London, United Kingdom
Search for more papers by this authorMounir Ait-Khaled,
Vincent C. Emery,
Corresponding Author
Mounir Ait-Khaled
Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill Street, London, United Kingdom
Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, United Kingdom===Search for more papers by this authorVincent C. Emery
Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill Street, London, United Kingdom
Search for more papers by this authorAbstract
Analysis of the HIV-1 V3 quasispecies present in an individual at the time of seroconversion was carried out. The polymerase chain reaction (PCR) was used to amplify proviral HIV-1 DNA extracted from peripheral blood mononuclear cells from a patient who was viraemic (p24 = 15 pg/ml) and had an equivocal HIV-1 antibody status. The PCR products were cloned and the DNA sequence determined for 15 clones. These data showed that the V3 region contained only limited sequence heterogeneity with a major variant accounting for 66% of the protein quasispecies present. The protein sequence of the principal neutralising domain on all species contained the relatively rare GPGKTL motif rather than GPGRAF. The relevance of these data for early stages of HIV infection are discussed.
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