Volume 57, Issue 5 pp. 1433-1442

Research Article

Macromolecular-Suppressed GABA-Edited MR Spectroscopy in the Posterior Cingulate Cortex of Patients With Acute Mild Traumatic Brain Injury

Andrei V. Manzhurtsev PhD,

Corresponding Author

Andrei V. Manzhurtsev PhD

[email protected]

orcid.org/0000-0001-5022-9952

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

Moscow State University, Moscow, Russian Federation

Address reprint requests to: A.V.M., Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, 22, Bolshaya Polyanka St., 119180 Moscow, Russian Federation. E-mail: [email protected]

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Alexey N. Yakovlev,

Alexey N. Yakovlev

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences, Moscow, Russian Federation

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Petr A. Bulanov,

Petr A. Bulanov

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Moscow State University, Moscow, Russian Federation

Philips Healthcare, Moscow, Russian Federation

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Petr E. Menshchikov PhD,

Petr E. Menshchikov PhD

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

Philips Healthcare, Moscow, Russian Federation

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Maxim V. Ublinskiy PhD,

Maxim V. Ublinskiy PhD

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

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Ilya A. Melnikov MD, PhD,

Ilya A. Melnikov MD, PhD

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

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Tolib A. Akhadov MD, PhD,

Tolib A. Akhadov MD, PhD

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Moscow State University, Moscow, Russian Federation

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Natalia A. Semenova PhD,

Natalia A. Semenova PhD

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

Moscow State University, Moscow, Russian Federation

N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences, Moscow, Russian Federation

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Andrei V. Manzhurtsev PhD,

Corresponding Author

Andrei V. Manzhurtsev PhD

[email protected]

orcid.org/0000-0001-5022-9952

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

Moscow State University, Moscow, Russian Federation

Address reprint requests to: A.V.M., Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, 22, Bolshaya Polyanka St., 119180 Moscow, Russian Federation. E-mail: [email protected]

Search for more papers by this author

Alexey N. Yakovlev,

Alexey N. Yakovlev

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences, Moscow, Russian Federation

Search for more papers by this author

Petr A. Bulanov,

Petr A. Bulanov

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Moscow State University, Moscow, Russian Federation

Philips Healthcare, Moscow, Russian Federation

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Petr E. Menshchikov PhD,

Petr E. Menshchikov PhD

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

Philips Healthcare, Moscow, Russian Federation

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Maxim V. Ublinskiy PhD,

Maxim V. Ublinskiy PhD

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

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Ilya A. Melnikov MD, PhD,

Ilya A. Melnikov MD, PhD

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

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Tolib A. Akhadov MD, PhD,

Tolib A. Akhadov MD, PhD

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Moscow State University, Moscow, Russian Federation

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Natalia A. Semenova PhD,

Natalia A. Semenova PhD

Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russian Federation

Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russian Federation

Moscow State University, Moscow, Russian Federation

N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences, Moscow, Russian Federation

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First published: 29 August 2022

https://doi.org/10.1002/jmri.28410

Citations: 1

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Abstract

Background

Mild traumatic brain injury (mTBI) causes a number of molecular and cellular alterations. There is evidence of an imbalance between the main excitatory (glutamate, Glu) and the main inhibitory (gamma-aminobutyric acid [GABA]) neurotransmitters following mTBI. In vivo human GABA–Glu balance studies following mTBI are sparse.

Purpose

To investigate the effect of acute mTBI on the GABA concentration measured in the posterior cingulate cortex (PCC) of pediatric patients by using the macromolecular (MM)-suppressed GABA J-editing technique.

Study type

Prospective patient and phantom.

Participants

A total of 14 pediatric patients (mean age 16.0 ± 1.7) with acute mTBI (<3 days after trauma; Glasgow Coma Scale 15) and 16 healthy volunteers (mean age 16.9 ± 2.8). Phantom: 524 cm³ sphere containing 10 mM glycine, 10 mM GABA.

Field Strength/Sequence

A 3 T, MEGA-PRESS pulse sequence.

Assessment

GABA spectra were processed in Gannet software. MM-suppressed GABA editing efficiency was derived from the phantom study. Absolute GABA and glutamate + glutamine (Glx) concentrations were quantified using different types of correction and compared between groups. N-acetyl aspartate (NAA) and choline (Cho) levels relative to tCr were also compared.

Statistical tests

Shapiro–Wilk test, Mann–Whitney U test, Student t-test, Pearson or Spearman correlations. P < 0.01 was considered statistically significant.

Results

The MM-suppressed GABA editing efficiency was 0.63. GABA signal fit error was <16% for all participants. The GABA concentration in the PCC of the mTBI group was significantly different from that in healthy controls: GABA/tCr was higher by 27%, absolute GABA concentration with different types of correction was higher by ≈17%. No significant differences were observed in Glx concentrations (P ≥ 0.32) or in Glx/tCr (P ≥ 0.1), NAA/tCr (P = 0.55), and Cho/tCr levels (P = 0.85).

Data conclusion

We report an increase in the GABA concentration in the PCC region in acute mTBI pediatric patients. This may suggest activation of GABA synthesis and impairment of the GABAergic system after acute mTBI.

Evidence Level

Technical Efficacy

Stage 1

Conflict of Interest

The authors declare no conflicts of interest

Supporting Information

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Citing Literature

Volume57, Issue5

May 2023

Pages 1433-1442

Macromolecular-Suppressed GABA-Edited MR Spectroscopy in the Posterior Cingulate Cortex of Patients With Acute Mild Traumatic Brain Injury

Abstract

Background

Purpose

Study type

Participants

Field Strength/Sequence

Assessment

Statistical tests

Results

Data conclusion

Evidence Level

Technical Efficacy

Conflict of Interest

Supporting Information

REFERENCES

Citing Literature

References

Information

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Macromolecular-Suppressed GABA-Edited MR Spectroscopy in the Posterior Cingulate Cortex of Patients With Acute Mild Traumatic Brain Injury

Abstract

Background

Purpose

Study type

Participants

Field Strength/Sequence

Assessment

Statistical tests

Results

Data conclusion

Evidence Level

Technical Efficacy

Conflict of Interest

Supporting Information

REFERENCES

Citing Literature

References

Related

Information