Correlation of dynamic contrast enhancement MRI parameters with microvessel density and VEGF for assessment of angiogenesis in breast cancer
Corresponding Author
Min-Ying Su PhD
Center for Functional Onco-Imaging and Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine, California
Min-Ying Su, Center for Functional Onco-Imaging, University of California, Irvine Hall 164, Irvine, CA 92697-5020
Yung-Liang Wan, First Department of Diagnostic Radiology, Change Gung Memorial Hospital at Linkou, College of Medicine and School of Medical Technology, Chang Gung University, 5 Fu-Hsing Road, Taoyuan, Taiwan 333
Search for more papers by this authorYun-Chung Cheung MD
Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Search for more papers by this authorHon Yu MS
Center for Functional Onco-Imaging and Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine, California
Search for more papers by this authorOrhan Nalcioglu PhD
Center for Functional Onco-Imaging and Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine, California
Search for more papers by this authorShin-Cheh Chen MD
Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Search for more papers by this authorSwei Hsueh MD
Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Search for more papers by this authorChristine E. McLaren PhD
Center for Functional Onco-Imaging and Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine, California
Search for more papers by this authorCorresponding Author
Yung-Liang Wan MD
Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Min-Ying Su, Center for Functional Onco-Imaging, University of California, Irvine Hall 164, Irvine, CA 92697-5020
Yung-Liang Wan, First Department of Diagnostic Radiology, Change Gung Memorial Hospital at Linkou, College of Medicine and School of Medical Technology, Chang Gung University, 5 Fu-Hsing Road, Taoyuan, Taiwan 333
Search for more papers by this authorCorresponding Author
Min-Ying Su PhD
Center for Functional Onco-Imaging and Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine, California
Min-Ying Su, Center for Functional Onco-Imaging, University of California, Irvine Hall 164, Irvine, CA 92697-5020
Yung-Liang Wan, First Department of Diagnostic Radiology, Change Gung Memorial Hospital at Linkou, College of Medicine and School of Medical Technology, Chang Gung University, 5 Fu-Hsing Road, Taoyuan, Taiwan 333
Search for more papers by this authorYun-Chung Cheung MD
Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Search for more papers by this authorHon Yu MS
Center for Functional Onco-Imaging and Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine, California
Search for more papers by this authorOrhan Nalcioglu PhD
Center for Functional Onco-Imaging and Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine, California
Search for more papers by this authorShin-Cheh Chen MD
Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Search for more papers by this authorSwei Hsueh MD
Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Search for more papers by this authorChristine E. McLaren PhD
Center for Functional Onco-Imaging and Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine, California
Search for more papers by this authorCorresponding Author
Yung-Liang Wan MD
Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Min-Ying Su, Center for Functional Onco-Imaging, University of California, Irvine Hall 164, Irvine, CA 92697-5020
Yung-Liang Wan, First Department of Diagnostic Radiology, Change Gung Memorial Hospital at Linkou, College of Medicine and School of Medical Technology, Chang Gung University, 5 Fu-Hsing Road, Taoyuan, Taiwan 333
Search for more papers by this authorAbstract
Purpose
To investigate the association between parameters obtained from dynamic contrast enhanced MRI (DCE-MRI) of breast cancer using different analysis approaches, as well as their correlation with angiogenesis biomarkers (vascular endothelial growth factor and vessel density).
Materials and Methods
DCE-MRI results were obtained from 105 patients with breast cancer (108 lesions). Three analysis methods were applied: 1) whole tumor analysis, 2) regional hot-spot analysis, and 3) intratumor pixel-by-pixel analysis. Early enhancement intensities and fitted pharmacokinetic parameters were studied. Paraffin blocks of 71 surgically resected specimens were analyzed by immunohistochemical staining to measure microvessel counts (with CD31) and vascular endothelial growth factor (VEGF) expression levels.
Results
MRI parameters obtained from the three analysis methods showed significant correlations (P < 0.0001), but a substantial dispersion from the linear regression line was noted (r = 0.72–0.97). The entire region of interest (ROI) vs. pixel population analyses had a significantly higher association compared to the entire ROI vs. hot-spot analyses. Cancer specimens with high VEGF expression had significantly higher CD31 microvessel densities than did specimens with low VEGF levels (P < 0.005). No significant association was found between MRI parameters obtained from the three analysis strategies and IHC based measurements of angiogenesis.
Conclusion
A consistent analysis strategy was important in the DCE-MRI study. In this series, none of these strategies yielded results for MRI based quantitation of tumor vascularity that were associated with IHC based measurements. Therefore, different analyses could not account for the lack of association. J. Magn. Reson. Imaging 2003;18:467–477. © 2003 Wiley-Liss, Inc.
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