Sealing of open dentinal tubules by laser irradiation: AFM and SEM observations of dentine surfaces
Corresponding Author
Roman Kubínek
Department of Experimental Physics, Faculty of Science, Palacký University, Olomouc, Czech Republic
Department of Experimental Physics, Faculty of Science, Palacký University, 17. listopadu 50, 771 46 Olomouc, Czech Republic.Search for more papers by this authorZdenka Zapletalová
1st Clinic of Stomatology, Medical Faculty, Palacký University, Olomouc, Czech Republic
Search for more papers by this authorMilan Vůjtek
Department of Experimental Physics, Faculty of Science, Palacký University, Olomouc, Czech Republic
Search for more papers by this authorRadko Novotný
Department of Microscopy Methods, Medical Faculty, Palacký University, Olomouc, Czech Republic
Search for more papers by this authorHana Kolářová
Department of Medical Biophysics, Medical Faculty, Palacký University, Olomouc, Czech Republic
Search for more papers by this authorHana Chmelíčková
Joint Laboratory of Optics, Palacký University and Institute of Physics of AS CR, Olomouc, Czech Republic
Search for more papers by this authorJan Peřina Jr
Joint Laboratory of Optics, Palacký University and Institute of Physics of AS CR, Olomouc, Czech Republic
Search for more papers by this authorCorresponding Author
Roman Kubínek
Department of Experimental Physics, Faculty of Science, Palacký University, Olomouc, Czech Republic
Department of Experimental Physics, Faculty of Science, Palacký University, 17. listopadu 50, 771 46 Olomouc, Czech Republic.Search for more papers by this authorZdenka Zapletalová
1st Clinic of Stomatology, Medical Faculty, Palacký University, Olomouc, Czech Republic
Search for more papers by this authorMilan Vůjtek
Department of Experimental Physics, Faculty of Science, Palacký University, Olomouc, Czech Republic
Search for more papers by this authorRadko Novotný
Department of Microscopy Methods, Medical Faculty, Palacký University, Olomouc, Czech Republic
Search for more papers by this authorHana Kolářová
Department of Medical Biophysics, Medical Faculty, Palacký University, Olomouc, Czech Republic
Search for more papers by this authorHana Chmelíčková
Joint Laboratory of Optics, Palacký University and Institute of Physics of AS CR, Olomouc, Czech Republic
Search for more papers by this authorJan Peřina Jr
Joint Laboratory of Optics, Palacký University and Institute of Physics of AS CR, Olomouc, Czech Republic
Search for more papers by this authorAbstract
Dentin of human teeth is a vital hydrated tissue. It is strongly sensitive to dehydration and drying that are commonly used in preparation of samples for scanning electron microscopy. Experience in examination of dentine surfaces of extracted human third molars using contact mode atomic force microscopy under moist conditions is described. The examined dentine surfaces are modified by laser radiation produced by a pulsed Nd:YAG laser that leads to sealing of open dentinal tubules under suitable conditions that are reached after covering dentine surfaces with dye agents. Out of four investigated dye agents erythrosin solution in water has been found the most suitable and the lower and upper limits of pulse energies for sealing of dentinal tubules have been set. Copyright © 2007 John Wiley & Sons, Ltd.
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