The ultrastructure of malpighian tubules and the chemical composition of the cocoon of Aeolothrips intermedius Bagnall (Thysanoptera)
Barbara Conti
Department of Tree Science, Entomology and Plant Pathology “G. Scaramuzzi,” University of Pisa, Pisa 56124, Italy
Search for more papers by this authorFrancesco Berti
Novartis Vaccines Research Center, Siena 53100, Italy
Search for more papers by this authorDavid Mercati
Department of Evolutionary Biology, University of Siena, Siena 53100, Italy
Search for more papers by this authorFabiola Giusti
Department of Evolutionary Biology, University of Siena, Siena 53100, Italy
Search for more papers by this authorCorresponding Author
Romano Dallai
Department of Evolutionary Biology, University of Siena, Siena 53100, Italy
Department of Evolutionary Biology, University of Siena, Via A. Moro 2, Siena I-53100, ItalySearch for more papers by this authorBarbara Conti
Department of Tree Science, Entomology and Plant Pathology “G. Scaramuzzi,” University of Pisa, Pisa 56124, Italy
Search for more papers by this authorFrancesco Berti
Novartis Vaccines Research Center, Siena 53100, Italy
Search for more papers by this authorDavid Mercati
Department of Evolutionary Biology, University of Siena, Siena 53100, Italy
Search for more papers by this authorFabiola Giusti
Department of Evolutionary Biology, University of Siena, Siena 53100, Italy
Search for more papers by this authorCorresponding Author
Romano Dallai
Department of Evolutionary Biology, University of Siena, Siena 53100, Italy
Department of Evolutionary Biology, University of Siena, Via A. Moro 2, Siena I-53100, ItalySearch for more papers by this authorAbstract
The secretory activity of the two branched malpighian tubules (MTs) of the second-instar larva in Aeolothrips intermedius is described. MTs of adult thrips have the typical ultrastructure of excretory epithelium with apical microvilli containing long mitochondria and a rich system of basal membrane infoldings. In the second-instar larva just before pupation, the ultrastructure of MT epithelial cells is dramatically different, and there are numerous huge Golgi systems in the cytoplasm. These cells are involved in an intense secretory activity to produce an electron-dense product which is released into the MTs lumen. This secretion is extruded from the hindgut and used by the second-instar larva to build an elaborate protective cocoon for pupation. Electron-spray-ionization mass spectrometry analysis of the cocoon revealed the presence of a β-N-acetyl-glucosamine, the main component of chitin, which is also present in the cocoons of Neuroptera and some Coleoptera. J. Morphol., 2010. © 2009 Wiley-Liss, Inc.
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