An alternative phase delivery system based on piston pumps and a back-pressure regulator has been developed for capillary column SFC. The chromatography is not affected by the fast piston pump refill. A homogeneous on-line mixing of binary phases with simultaneous pressure programming is easily accessible without any additional computing. Acceptable reproducibilities (< 3.5% RSD for external and < 2.0% RSD for internal standard methods) were found with mixtures of 2-propanol/CO₂ as mobile phases using UV detection and split ratios of 1:60 and 1:120. Variation and control of the split are easily done by simple flow rate volumetric changes.

References

1 K. E. Markides and M. L. Lee, “ SFC Applications”, Workshop on SFC, Provo, USA, January 12–14, 1988, Brigham Young University Press (1988).
Google Scholar
2 H. H. Lauer, D. McManigill, and R. D. Board, Anal. Chem. 55 (1983) 1370–75.
10.1021/ac00259a041
CAS Web of Science® Google Scholar
3 K. Grolimund, W. P. Jackson, M. Joppich, W. Nussbaum, K. Anton, and H. M. Widmer, in D. Ishii, K. Jinno, and P. Sandra (Eds.), “ Proceedings 7th Int. Symp. on Capillary Chromatography”, Gifu, May 11–14, 1986, The University of Nagoya Press, Nagoya, Japan, (1986), p. 625–636.
Google Scholar
4 J. C. Kuei, K. E. Markides, and M. L. Lee, HRC & CC 10 (1987) 257–262.
10.1002/jhrc.1240100509
CAS Web of Science® Google Scholar
5 R. D. Smith, B. W. Wright, and C. R. Yonker, Anal. Chem. 60 (1988) 1323A–1336A.
10.1021/ac00174a720
CAS PubMed Web of Science® Google Scholar
6 C. R. Yonker and R. D. Smith, J. Chromatogr. 361 (1986) 25–32.
10.1016/S0021-9673(01)86890-4
CAS Web of Science® Google Scholar
7 C. R. Yonker, D. G. McMinn, B. W. Wright, and R. D. Smith, J. Chromatogr. 396 (1987) 19–29.
10.1016/S0021-9673(01)94039-7
CAS Web of Science® Google Scholar
8 S. M. Fields, K. E. Markides, and M. L. Lee, J. Chromatogr. 406 (1987) 223–235.
10.1016/S0021-9673(00)94031-7
CAS Web of Science® Google Scholar
9 S. M. Fields, K. E. Markides, and M. L. Lee, HRC & CC 11 (1988), 25–29.
10.1002/jhrc.1240110108
CAS Web of Science® Google Scholar
10 S. Schmidt, L. G. Blomberg, and E. R. Campbell, Chromatographia 25 (1988) 775–780.
10.1007/BF02262083
CAS Web of Science® Google Scholar
11 K. Anton, N. Pericles, S. M. Fields, and H. M. Widmer, Chromatographia 26 (1989) 224–228.
10.1007/BF02268156
Web of Science® Google Scholar
12 C. R. Yonker and R. D. Smith, J. Phys. Chem. 92 (1988) 2374–2378.
10.1021/j100319a054
CAS Web of Science® Google Scholar
13 A. Giorgetti, N. Pericles, K. Anton, P. Dätwyler, and H. M. Widmer, J. Chromatogr. Sci., in press.
Google Scholar
14 C. R. Yonker and R. D. Smith, Anal. Chem. 59 (1987) 727–731.
10.1021/ac00132a010
CAS Web of Science® Google Scholar
15 A. E. Bruno, E. Gassmann, N. Pericles, and K. Anton, Anal. Chem. 61 (1989) 876–883.
10.1021/ac00183a019
CAS Web of Science® Google Scholar
16 M. R. Andersen, LC-GC 6 (1988) 566–570.
CAS Web of Science® Google Scholar
17 J. C. Fjeldsted and M. L. Lee, Anal. Chem. 56 (1984) 619A–628A.
10.1021/ac00268a004
CAS Web of Science® Google Scholar
18 D. W. Later, D. J. Bornhop, E. D. Lee, J. D. Henion, and R. C. Wieboldt, LC-GC 5 (1987) 804–816.
CAS Google Scholar
19 S. M. Fields, K. E. Markides, and M. L. Lee, Anal. Chem. 60 (1988) 802–806.
10.1021/ac00159a014
CAS Web of Science® Google Scholar
20 “ Gas encyclopaedia”, L'Air Liquide, Elsevier, Amsterdam, Netherlands (1976), p. 338.
Google Scholar
21 J. Köhler, A. Rose, and G. Schomburg, HRC & CC 11 (1988) 191–197.
10.1002/jhrc.1240110210
Web of Science® Google Scholar
22 R. C. Weast (Ed.), “ CRC Handbook of Chemistry and Physics”, 66th edition, CRC Press Inc., Boca Raton, Florida, USA, (1985) p. C447.
Web of Science® Google Scholar
23 B. W. Wright and R. D. Smith, J. Chromatogr. 355 (1986) 367–373.
10.1016/S0021-9673(01)97340-6
CAS Web of Science® Google Scholar
24 B. E. Richter, D. E. Knowles, M. R. Andersen, N. L. Porter, E. R. Campbell, and D. W. Later, HRC & CC 11 (1988) 29–32.
10.1002/jhrc.1240110109
CAS Web of Science® Google Scholar

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Volume12, Issue6

June 1989

Pages 394-399

Capillary column SFC instrumentation based on piston pumps with a programmable back-pressure regulator for on-line mixing of mobile phases

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Capillary column SFC instrumentation based on piston pumps with a programmable back-pressure regulator for on-line mixing of mobile phases

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