Identification of trypsin-like activity in sturgeon spermatozoa
Andrzej Ciereszko
School of Natural Resources, The Ohio State University, Columbus, Ohio 43210
Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, 10-718 Olsztyn, Poland
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Konrad Dabrowski
School of Natural Resources, The Ohio State University, Columbus, Ohio 43210
The Ohio State University, School of Natural Resources, 210 Kottman Hall, 2021 Coffey Rd., Columbus, OH 43210===Search for more papers by this authorFeng Lin
School of Natural Resources, The Ohio State University, Columbus, Ohio 43210
Search for more papers by this authorSerge I. Doroshov
Department of Animal Sciences, University of California, Davis, California 95616
Search for more papers by this authorAndrzej Ciereszko
School of Natural Resources, The Ohio State University, Columbus, Ohio 43210
Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, 10-718 Olsztyn, Poland
Search for more papers by this authorCorresponding Author
Konrad Dabrowski
School of Natural Resources, The Ohio State University, Columbus, Ohio 43210
The Ohio State University, School of Natural Resources, 210 Kottman Hall, 2021 Coffey Rd., Columbus, OH 43210===Search for more papers by this authorFeng Lin
School of Natural Resources, The Ohio State University, Columbus, Ohio 43210
Search for more papers by this authorSerge I. Doroshov
Department of Animal Sciences, University of California, Davis, California 95616
Search for more papers by this authorAbstract
We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N-α-p-tosyl-L-lysine chloromethyl ketone-HCl (TLCK), but not by N-tosyl-L-phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin-like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin-like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin-like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley-Liss, Inc.
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