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Influence of tissue composition on the final volume of rat liver blocks prepared for electron microscopy
John F. Bertram,
Robert P. Bolender,
Paul D. Sampson,
John F. Bertram
Department of Biological Structure, University of Washington, Seattle, Washington 98195
Search for more papers by this authorRobert P. Bolender
Department of Biological Structure, University of Washington, Seattle, Washington 98195
Search for more papers by this authorPaul D. Sampson
Department of Statistics, University of Washington, Seattle, Washington 98195
Search for more papers by this authorJohn F. Bertram,
Robert P. Bolender,
Paul D. Sampson,
John F. Bertram
Department of Biological Structure, University of Washington, Seattle, Washington 98195
Search for more papers by this authorRobert P. Bolender
Department of Biological Structure, University of Washington, Seattle, Washington 98195
Search for more papers by this authorPaul D. Sampson
Department of Statistics, University of Washington, Seattle, Washington 98195
Search for more papers by this authorAbstract
Methods for measuring and evaluating changes in volume of small tissue blocks prepared for transmission electron microscopy are presented. The results indicate: (1) that some blocks swell and others shrink, and (2) that changes in block volume can be explained by inhomogeneous changes occurring in three different tissue compartments.
For stereological studies attempting to extrapolate changes in fixed and embedded tissue to those of fresh tissue, consequences of inhomogeneous volume changes in tissue compartments include a decrease in reliability and an increase in statistical variance of stereological density estimates. Since factors could not be found to correct for the changes in individual tissue compartments, alternative strategies are considered for dealing with the volume artifacts of fixation.
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