Concurrent environmental enrichment and chronic restraint stress: Effects on innate anxiety and depressive-like behavior in male adolescent mice
Yu Mao
Scientific Research Center, Yunnan University of Chinese Medicine, Kunming, P.R. China
Search for more papers by this authorYanmei Chen
Medical Faculty, Kunming University of Science & Technology, Kunming, P.R. China
Search for more papers by this authorXiaomi Yang
Scientific Research Center, Yunnan University of Chinese Medicine, Kunming, P.R. China
Search for more papers by this authorJianhong Wang
Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P.R. China
Search for more papers by this authorLongjiao Ge
Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P.R. China
Search for more papers by this authorJuan Fu
College of Biological and Environmental Engineering, Binzhou University, Binzhou, P.R. China
Search for more papers by this authorCorresponding Author
Huaying Sun
Scientific Research Center, Yunnan University of Chinese Medicine, Kunming, P.R. China
The Key Laboratory of External Drug Delivery System and Preparation Technology in University of Yunnan Province, School of Chinese Materia Medica, Yunnan Univertity of Chinese Medicine, Kunming, P.R. China
Correspondence
Huaying Sun, Yunnan University of Chinese Medicine, Kunming, P.R. China.
Email: [email protected]
Yuanye Ma, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Yuanye Ma
Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P.R. China
Correspondence
Huaying Sun, Yunnan University of Chinese Medicine, Kunming, P.R. China.
Email: [email protected]
Yuanye Ma, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
Email: [email protected]
Search for more papers by this authorYu Mao
Scientific Research Center, Yunnan University of Chinese Medicine, Kunming, P.R. China
Search for more papers by this authorYanmei Chen
Medical Faculty, Kunming University of Science & Technology, Kunming, P.R. China
Search for more papers by this authorXiaomi Yang
Scientific Research Center, Yunnan University of Chinese Medicine, Kunming, P.R. China
Search for more papers by this authorJianhong Wang
Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P.R. China
Search for more papers by this authorLongjiao Ge
Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P.R. China
Search for more papers by this authorJuan Fu
College of Biological and Environmental Engineering, Binzhou University, Binzhou, P.R. China
Search for more papers by this authorCorresponding Author
Huaying Sun
Scientific Research Center, Yunnan University of Chinese Medicine, Kunming, P.R. China
The Key Laboratory of External Drug Delivery System and Preparation Technology in University of Yunnan Province, School of Chinese Materia Medica, Yunnan Univertity of Chinese Medicine, Kunming, P.R. China
Correspondence
Huaying Sun, Yunnan University of Chinese Medicine, Kunming, P.R. China.
Email: [email protected]
Yuanye Ma, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Yuanye Ma
Kunming Primates Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P.R. China
Correspondence
Huaying Sun, Yunnan University of Chinese Medicine, Kunming, P.R. China.
Email: [email protected]
Yuanye Ma, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
Email: [email protected]
Search for more papers by this authorFunding information
This study was supported by grant from National Science Foundation of China (81703497, 31771194), National Key R&D Program of China (2018YFC1313803), scientific research fund project of Yunnan Provincial Department of Education (2019J1069) and the Doctor Foundation of Binzhou University (2016Y18)
Abstract
Adolescence is a period that exhibits both vulnerability and adaptation to environmental stimulus. This study explored the co-existence effect of environmental enrichment (EE) and restraint stress (RS) on innate anxiety and depressive-like behavior in adolescent mice. Male ICR mice were treated with daily EE and RS (4 h/d or 8 h/d) for 2 or 4 weeks from early adolescence (postnatal day 30) and emotional behaviors were evaluated 24 h after the end of treatment. 4 weeks of 8 h RS treatment decreased immobility time in forced swimming test, demonstrating an antidepressant-like effect. For 2 weeks of treatment, 8 h RS significantly reduced the time spent in the lighted compartment of the light-dark box, indicating an increased anxiety level. These results show that under the present experimental design, RS treatment with different duration could have different effect on mice emotion-related behavior, but there was no interaction between EE and RS.
CONFLICT OF INTEREST
The authors declare that they have no competing interests.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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