Modulation of the immunophenotype of ovarian cancer cells by N,N-dimethylformamide and transforming growth factor-β1
Corresponding Author
Thomas W. Grunt
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, AustriaSearch for more papers by this authorHelga Oeller
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorCanatay Somay
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorEvelyn Dittrich
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorBarbara Fazeny
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorChristine Mannhalter
Division of Molecular Genetics, Department for Medical and Chemical Laboratory Diagnosis, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorChristian Dittrich
Ludwig Boltzmann-Institute for Applied Cancer Research, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorCorresponding Author
Thomas W. Grunt
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, AustriaSearch for more papers by this authorHelga Oeller
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorCanatay Somay
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorEvelyn Dittrich
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorBarbara Fazeny
Laboratory for Applied and Experimental Tumor Cell Biology, Division of Oncology, Department of Internal Medicine I, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorChristine Mannhalter
Division of Molecular Genetics, Department for Medical and Chemical Laboratory Diagnosis, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorChristian Dittrich
Ludwig Boltzmann-Institute for Applied Cancer Research, University of Vienna, A-1090 Vienna, Austria
Search for more papers by this authorAbstract
Exposure of HOC-7 ovarian adenocarcinoma cells to regulators of cell differentiation caused inducer-dependent alterations of the antigenic pattern of the cells. Immunocytochemistry revealed that N,N-dimethylformamide (DMF) elevated the membrane staining for epidermal growth factor (EGF)-receptor and for desmoplakins I and II. DMF also stimulated cytoplasmic and surface labeling for CA 125 and the deposition of fibronectin into the extracellular matrix. Stimulation of fibronectin was also seen after addition of transforming growth factor (TGF)-β1. These responses were quantified using a fixed-cell, enzyme-linked immunosorbent assay (ELISA) and revealed that DMF dose-dependently induced expression of EGF-receptor, CA 125, fibronectin, and desmoplakins I and II. TGF-β1 stimulated fibronectin and desmoplakins I and II only. Production of EGF and TGF-α was not affected by these inducers. Immunocytochemistry, ELISA and Western blotting showed that both inducers caused down-regulation of myc oncoproteins. DMF was more effective in changing the immunophenotype of HOC-7 cells than TGF-β1. Desmoplakins I and II demonstrated elevated epithelial differentiation, whereas fibronectin indicated stimulation of extracellular matrix formation. Elevated EGF-receptor could not compensate for the growth inhibition induced by DMF. The expression of myc oncoproteins was inversely related to cell proliferation. CA 125, however, seems to be unrelated to cell growth. © 1993 Wiley-Liss, Inc.
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