Simultaneous expression of type I procollagen mRNA and albumin in cirrhotic human liver
Corresponding Author
Dr. Shuhei Yamada
3rd Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan
The 2nd Department of Internal Medicine, Shinshu University School of Medicine, 3–1–1 Asahi, Matsumoto Nagano Pref. 390 JapanSearch for more papers by this authorTakehiko Kohi
3rd Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan
Search for more papers by this authorRyu-Ichiro Hata
Department of Tissue Physiology, Medical Research Institute, Tokyo Medical and Dental University, Kanda-Surugadai, Chiyoda-ku, Tokryo, Japan
Search for more papers by this authorSetsuo Hirohashi
Department of Biochemical Genetics, Medical Research Institute, Tokyo Medical and Dental University, Kanda-Surugadai, Chiyoda-ku, Tokryo, Japan
Search for more papers by this authorShun-Ichi Kurata
Patho. Division, National cancer Center Research Institute, Tsukiji, Chou-ku, Tokyo, Japan
Search for more papers by this authorHaruki Senoo
1st Department of Anatomy, School of Medicine, Tokyo Medical and Dental University, 1–5–45 Yushima, Bunkyo-ku, Tokyo, Japan
Search for more papers by this authorKooko Sakakibara
Department of Carcinogenesis and Cancer Susceptibility, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan
Search for more papers by this authorToshihko Hayashi
Department of Chemistry, College of Arts and Science, University of Tokyo, Tokyo 153, Japan
Search for more papers by this authorPaul K. Nakane
3rd Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan
Search for more papers by this authorCorresponding Author
Dr. Shuhei Yamada
3rd Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan
The 2nd Department of Internal Medicine, Shinshu University School of Medicine, 3–1–1 Asahi, Matsumoto Nagano Pref. 390 JapanSearch for more papers by this authorTakehiko Kohi
3rd Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan
Search for more papers by this authorRyu-Ichiro Hata
Department of Tissue Physiology, Medical Research Institute, Tokyo Medical and Dental University, Kanda-Surugadai, Chiyoda-ku, Tokryo, Japan
Search for more papers by this authorSetsuo Hirohashi
Department of Biochemical Genetics, Medical Research Institute, Tokyo Medical and Dental University, Kanda-Surugadai, Chiyoda-ku, Tokryo, Japan
Search for more papers by this authorShun-Ichi Kurata
Patho. Division, National cancer Center Research Institute, Tsukiji, Chou-ku, Tokyo, Japan
Search for more papers by this authorHaruki Senoo
1st Department of Anatomy, School of Medicine, Tokyo Medical and Dental University, 1–5–45 Yushima, Bunkyo-ku, Tokyo, Japan
Search for more papers by this authorKooko Sakakibara
Department of Carcinogenesis and Cancer Susceptibility, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan
Search for more papers by this authorToshihko Hayashi
Department of Chemistry, College of Arts and Science, University of Tokyo, Tokyo 153, Japan
Search for more papers by this authorPaul K. Nakane
3rd Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan
Search for more papers by this authorAbstract
The gene expression of human type 1 procollagen was investigated in cirrhotic human liver by using in situ hybridization with nonradioactive DNA probes. Using in situ hybridizaiton can provide direct evidence for the cell type capable for type I collagen synthesis in tissues. T-T dimerized DNA probes were used and DNAs hybridized in situ were detected immunohistochemically using specific antibodies against T-T dimer. The data demonstrated that type I collagen is synthesized in hepatocyles and stellate cells in pseudolobules and in fibroblasts in Glissons capsules in cirrhotic human livers. we indicated hepatocytes morphologically and functionally by using immunohistochemical localization of albumin, which ws used as a marker of hepatocyte, since albumin is synthesized exclusively by hepatocytes. © 1992 Wiley-Liss, Inc.
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