New model for bone resorption study in vitro: Human osteoclast-like cells from giant cell tumors of bone
Maria Grano
Institute of Human Anatomy, University of Bari, Bari, Italy
Istituto di Citomorfologia Normale e Patologica di Chieti, Istituto Ortopedico Rizzoli, Bologna, Italy
Search for more papers by this authorSilvia Colucci
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorMichele De Bellis
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorPaola Zigrino
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorLuigi Argentino
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorGiovanni Zambonin
Institute of Human Anatomy, University of Bari, Bari, Italy
I Clinica Ortopedica, University of Bari, Bari, Italy
Search for more papers by this authorMassimo Serra
Laboratorio di Ricerca Oncologica, Istituto Ortopedico Rizzoli, Bologna, Italy
Search for more papers by this authorKatia Scotlandi
Laboratorio di Ricerca Oncologica, Istituto Ortopedico Rizzoli, Bologna, Italy
Search for more papers by this authorAnna Teti
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorCorresponding Author
Prof. Alberta Zambonin Zallone
Institute of Human Anatomy, University of Bari, Bari, Italy
Istituto di Anatomia Umana Normale Policlinico Piazza G. Cesare 70124 Bari, ItalySearch for more papers by this authorMaria Grano
Institute of Human Anatomy, University of Bari, Bari, Italy
Istituto di Citomorfologia Normale e Patologica di Chieti, Istituto Ortopedico Rizzoli, Bologna, Italy
Search for more papers by this authorSilvia Colucci
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorMichele De Bellis
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorPaola Zigrino
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorLuigi Argentino
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorGiovanni Zambonin
Institute of Human Anatomy, University of Bari, Bari, Italy
I Clinica Ortopedica, University of Bari, Bari, Italy
Search for more papers by this authorMassimo Serra
Laboratorio di Ricerca Oncologica, Istituto Ortopedico Rizzoli, Bologna, Italy
Search for more papers by this authorKatia Scotlandi
Laboratorio di Ricerca Oncologica, Istituto Ortopedico Rizzoli, Bologna, Italy
Search for more papers by this authorAnna Teti
Institute of Human Anatomy, University of Bari, Bari, Italy
Search for more papers by this authorCorresponding Author
Prof. Alberta Zambonin Zallone
Institute of Human Anatomy, University of Bari, Bari, Italy
Istituto di Anatomia Umana Normale Policlinico Piazza G. Cesare 70124 Bari, ItalySearch for more papers by this authorAbstract
Cells harvested from 12 human giant cell tumors of bone and kept in culture for several passages were characterized for bone-resorbing capability, total and tartrate-resistant acid phosphatase activity, response to the calciotropic hormone calcitonin, cell proliferation, multinucleation after passages, and presence of calcium sensing. Cells obtained from three tumors presented a complete panel of osteoclast characteristics and maintained their multinuclearity after several passages. Cells from four other tumors increased their cAMP levels after treatment with calcitonin, and the other five apparently consisted of cells of stromal origin. These human cell populations with osteoclast characteristics may provide valid in vitro models for the investigation of osteoclastic differentiation and activity.
References
- 1 Jaffe HL, Lichtenstein L, Portis RB 1940 Giant cell tumor of bone. Its pathologic appearance, grading, supposed variants, and treatment. Arch Pathol 30: 993–1031.
- 2 Johnston J 1977 Giant cell tumor of bone. The role of the giant cell in orthopaedic pathology. Orthop Clin North Am 8: 751–770.
- 3
Dahlin DC,
Cupps RE,
Johnson EW Jr
1970
Giant cell tumor: A study of 195 cases.
Cancer
25:
1061–1070.
10.1002/1097-0142(197005)25:5<1061::AID-CNCR2820250509>3.0.CO;2-E CAS PubMed Web of Science® Google Scholar
- 4 Goldenberg RR, Campbell CJ, Bonofiglio M 1970 Giant cell tumor of bone: An analysis of two hundred and eighteen cases. J Bone Joint Surg [Am] 52: 619–664.
- 5 Larsson SE, Lorentzon R, Boquist L 1975 Giant-cell tumor of bone: A demographic, clinical, and histopathological study of all cases recorded in the Swedish Cancer Registry for the years 1958 through 1968. J Bone Joint Surg [Am] 57: 167–173.
- 6 McGrath PJ 1972 Giant-cell tumour of bone: An analysis of fifty-two cases. J Bone Joint Surg [Br] 54: 213–229.
- 7 Shifrin LZ 1972 Giant cell tumor of bone. Clin Orthop 82: 59–66.
- 8 Horton MA, Lewis D, McNulty K, Pringle JAS, Chambers TJ 1985 Monoclonal antibodies to osteoclastomas (giant cell bone tumors): Definition of osteoclast-specific cellular antigens. Cancer Res 45: 5663–5669.
- 9 Schajowicz F 1981 Giant cell tumor (osteoclastoma). In: F Schajowicz (ed) Tumors and Tumor-Bone Lesions of Bone and Joint. Springer-Verlag, New York, pp. 205–239.
- 10 Aparisi T, Arborgh B, Ericsson JLE 1977 Giant cell tumor of bone: Detailed fine structural analysis of different cell components. Virchows Arch [A] 376: 273–298.
- 11 Aparisi T, Arborgh B, Ericsson JLE 1977 Giant cell tumor of bone. Fine structural localization of acid phosphatase. Virchows Arch [A] 376: 299–308.
- 12
Hanaoka H,
Friedman B,
Mack RP
1970
Ultrastructure and histogenesis of giant-cell tumor of bone.
Cancer
25:
1408–1423.
10.1002/1097-0142(197006)25:6<1408::AID-CNCR2820250622>3.0.CO;2-# CAS PubMed Web of Science® Google Scholar
- 13 Jeffree GM, Price CHG 1965 Bone tumors and their enzymes: A study of the phosphatases, non-specific esterases and beta-glucuronidase of osteogenic and cartilaginous tumors, fibroblastic and giant-cell lesions. J Bone Joint Surg [Br] 47: 120–136.
- 14 Steiner GC, Ghosh L, Dorfman HD 1972 Ultrastructure of giant cell tumors of bone. Hum Pathol 3: 569–586.
- 15 Goldring SR, Schiller AL, Mankin HJ, Dayer JM, Krane SM 1986 Characterization of cells from human giant cell tumors of bone. Clin Orthop 204: 59–75.
- 16 Goldring SR, Roelke MS, Petrison KK, Bhan AK 1987 Human giant cell tumors of bone. Identification and characterization of cell types. J Clin Invest 79: 483–491.
- 17 Joyner CJ, Quinn JM, Triffitt JT, Owen ME, Athanasou NA 1992 Phenotypic characterization of mononuclear and multinucleated cells of giant cell tumour of bone. Bone Miner 16: 37–48.
- 18 Burmester GR, Winchester RJ, Dimitriu-Bona A, Klein M, Steiner G, Sissons HA 1983 Delineation of four cell types comprising the giant cell tumor of bone. Expression of 1a and monocyte-macrophage lineage antigens. J Clin Invest 71: 1633–1648.
- 19 Komiya S, Sasaguri Y, Inoue A, Nakashima M, Yamamoto S, Yanagida I, Morimatsu M 1990 Characterization of cells cultured from human giant-cell tumors of bone. Clin Orthop 258: 304–309.
- 20 Campanacci M, Bagnara GP, Serra M, Giovannini M, Tomasi P, Pileri S, Poggi S, Lollini PL, Picci P, and Paolucci G 1989 Giant cell tumor of bone: A model for the in vitro human osteoclast characterization. Tumori 75: 389–395.
- 21 Lowry OH, Rosembrough NJ, Farr AL, Randall RJ 1951 Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275.
- 22 Blair HC, Kahn AJ, Crouch EC, Jeffrey JJ, Teitelbaum SL 1986 Isolated osteoclasts resorb the organic and inorganic components of bone. J Cell Biol 102: 1164–1172.
- 23 Arnett TR, Dempster DW 1987 A comparative study of disaggregated chick and rat osteoclasts in vitro: Effects of calcitonin and prostaglandins. Endocrinology 120: 602–608.
- 24 Grynkiewicz G, Poenie M, Tsien RY 1985 A new generation of Ca++ indicators with greatly improved fluorescence properties. J Biol Chem 260: 3440–3450.
- 25 Oreffo ROC, Teti A, Triffitt JT, Francis MJO, Carano A, Zallone Zambonin A 1988 Effect of vitamin A on bone resorption: Evidence for direct stimulation of isolated chick osteoclasts by retinol and retinoic acid. J Bone Miner Res 3: 203–210.
- 26 Malgaroli A, Meldolesi J, Zallone Zambonin A, Teti A 1989 Control of cytosolic free calcium in rat and chicken osteoclasts. The role of extracellular calcium and calcitonin. J Biol Chem 264: 14342–14347.
- 27 Teti A, Zallone Zambonin A 1992 Synergistic control of osteoclast activity by calcitonin and extracellular calcium concentration. Ital J Miner Electrolyte Metab 6: 17–21.
- 28 Lindunger A, MacKay CA, Ek-Rylander B, Andersson G, Marks SC Jr 1990 Histochemistry and biochemistry of tartrate-resistant acid phosphatase (TRAP) and tartrate-resistant acid adenosine triphosphate (TrATPase) in bone, bone marrow and spleen: Implications for osteoclast ontogeny. Bone Miner 10: 109–119.
- 29 Chambers TJ, Magnus CJ 1982 Calcitonin alters behaviour of isolated osteoclasts. J Pathol 136: 27–39.
- 30 Zaidi M, Datta HK, Patchell A, Moonga B, MacIntyre I 1989 Calcium-activated intracellular calcium elevation: A novel mechanism of osteoclast regulation. Biochem Biophys Res Commun 163: 1461–1465.
- 31 Miyauchi A, Hruska KA, Greenfield EM, Duncan R, Alvarez J, Barattolo R, Colucci S, Zallone Zambonin A, Teitelbaum SL, Teti A 1990 Osteoclast cytosolic calcium, regulated by voltage-gated calcium channels and extracellular calcium, controls podosome assembly and bone resorption. J Cell Biol 111: 2543–2552.
- 32 Ohsaki Y, Takahashi S, Scarcez T, Demulder A, Nishihara T, Williams R, Roodman GD 1992 Evidence for an autocrine/paracrine role for interleukin-6 in bone resorption by giant cells from giant cell tumors of bone. Endocrinology 131: 2229–2234.
