Growth and repair of cartilage: Organ culture system utilizing chondroprogenitor cells of condylar cartilage in newborn mice
Anna Weiss
Laboratory for Musculoskeletal Research, The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine Technion—Israel Institute of Technology, Haifa, Israel
Search for more papers by this authorErella Livne
Laboratory for Musculoskeletal Research, The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine Technion—Israel Institute of Technology, Haifa, Israel
Search for more papers by this authorKlaus von der Mark
Laboratory for Connective Tissue Research, The Max-Planck-Institut for Biochemistry, Martinsried, FRG
Search for more papers by this authorDick Heinegard
Department of Physical Chemistry, University of Lund, Lund, Sweden
Search for more papers by this authorCorresponding Author
Michael Silbermann
Laboratory for Musculoskeletal Research, The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine Technion—Israel Institute of Technology, Haifa, Israel
The Rappaport Family Institute for Research in the Medical Sciences P.O. Box 9649 Haifa, 31096 IsraelSearch for more papers by this authorAnna Weiss
Laboratory for Musculoskeletal Research, The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine Technion—Israel Institute of Technology, Haifa, Israel
Search for more papers by this authorErella Livne
Laboratory for Musculoskeletal Research, The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine Technion—Israel Institute of Technology, Haifa, Israel
Search for more papers by this authorKlaus von der Mark
Laboratory for Connective Tissue Research, The Max-Planck-Institut for Biochemistry, Martinsried, FRG
Search for more papers by this authorDick Heinegard
Department of Physical Chemistry, University of Lund, Lund, Sweden
Search for more papers by this authorCorresponding Author
Michael Silbermann
Laboratory for Musculoskeletal Research, The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine Technion—Israel Institute of Technology, Haifa, Israel
The Rappaport Family Institute for Research in the Medical Sciences P.O. Box 9649 Haifa, 31096 IsraelSearch for more papers by this authorAbstract
The zone of progenitor cells of mandibular condyles of neonatal mice was kept in an organ culture system for up to 8 days. Qualitative and quantitative determinations indicated a pronounced proliferative activity during the initial phases of the culture followed by a differentiation phase and the acquisition of typical hyaline cartilage. The mature hypertrophic chondrocytes were found to be surrounded by cartilage-specific macromolecules such as type II collagen, cartilage proteoglycans, and cartilage anchorin. The extracellular mineralization proceeded along matrix vesicles as is usually noted in vivo. A unique finding in this study was the observation that explants comprising cartilage progenitor cells and their adjacent extracellular matrix succeeded in repairing the damaged condylar in vitro.
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