Biogenic Calcium Mineral-Chitosan Composite Nanofiber Dressing for Control of Traumatic Hemorrhage
Pranabesh Kumar Sasmal
Department of Mechanical Engineering, Jadavpur University, Kolkata, West Bengal, India
Search for more papers by this authorShalini Dasgupta
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Howrah, West Bengal, India
Search for more papers by this authorJerzy Kubacki
Institute of Physics, University of Silesia, and Silesian Center for Education and Interdisciplinary Research, Chorzów, Poland
Search for more papers by this authorSamsamul Hoque
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India
Search for more papers by this authorSamit Kumar Nandi
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India
Search for more papers by this authorAbhijit Chanda
Department of Mechanical Engineering, Jadavpur University, Kolkata, West Bengal, India
Search for more papers by this authorCorresponding Author
Pallab Datta
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Kolkata, West Bengal, India
Correspondence:
Pallab Datta ([email protected]; [email protected])
Search for more papers by this authorPranabesh Kumar Sasmal
Department of Mechanical Engineering, Jadavpur University, Kolkata, West Bengal, India
Search for more papers by this authorShalini Dasgupta
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Howrah, West Bengal, India
Search for more papers by this authorJerzy Kubacki
Institute of Physics, University of Silesia, and Silesian Center for Education and Interdisciplinary Research, Chorzów, Poland
Search for more papers by this authorSamsamul Hoque
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India
Search for more papers by this authorSamit Kumar Nandi
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India
Search for more papers by this authorAbhijit Chanda
Department of Mechanical Engineering, Jadavpur University, Kolkata, West Bengal, India
Search for more papers by this authorCorresponding Author
Pallab Datta
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Kolkata, West Bengal, India
Correspondence:
Pallab Datta ([email protected]; [email protected])
Search for more papers by this authorFunding: The authors received no specific funding for this work.
ABSTRACT
Effective biomaterials for traumatic hemorrhage control remain an unmet clinical challenge, while several potential biomaterials, like oyster shell waste, are emerging as key ecological hazards. Hemostatic nanofiber dressing using biogenic calcium powders from Magallana cuttackensis is reported here. Extracted and treated powders were characterized by XRD, EDX, XPS, and Raman spectroscopy to confirm the presence of CaO and Ca(OH)2 as the calcium phases. A chitosan-based nanofiber matrix, one of the most commonly used hemostatic dressing materials, was used as a control nanofiber (CNF) and integrated with shell waste calcium minerals (MSWNF). The hemolysis of CNF and MSWNF nanofibers was 5% ± 0.3% and 4% ± 0.2%, respectively. The in vitro clotting time (CT) under CNF and MSWNF nanofibers was 230 ± 11 s and 198 ± 4 s, which, along with other parameters—prothrombin times (27 ± 0.4 s and 22 ± 0.6 s) and plasma recalcification time (76 ± 4 s and 47 ± 1 s)—indicated enhancement in hemostasis performance by MSWNF over CNF. After in vivo evaluation in the rabbit incision model, the bleeding time of the control group (274 ± 6 s) was longer than CNF (97 ± 8 s) or MSWNF (75 ± 5 s). Blood oozing until hemostasis was 0.380 g, 0.354 g, and 0.121 g under control gauge, CNF, and MSWNF, respectively, demonstrating enhancement in hemostasis performance by MSWNF. Further, histopathological examination proved continued epithelialization and formation of capillaries, indicative of wound healing. The results provide promising evidence for a more effective biomaterial from biogenic oyster shell waste than commonly employed hemostatic dressing materials.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
Filename | Description |
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jbmb35601-sup-0001-Figures.docxWord 2007 document , 4.8 MB |
Figure S1. The morphological appearances of marine shell waste powder obtained after extraction (MSW-O), marine shell waste powder extracted and calcined at 1000°C (MSW-T), control nanofibers (CNF), and nanofibers loaded with calcined marine shell waste powders (MSWNF), and MSWNF after sterilization before administration into animals. Figure S2. The antibacterial activity of marine shell waste powder obtained after extraction (MSW-O), marine shell waste powder extracted and calcined at 1000°C (MSW-T), control nanofibers (CNF), and nanofibers loaded with calcined marine shell waste powders (MSWNF) against (a) S. aureus and (b) E. coli. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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