Effects of the intranasal application of gold nanoparticles on the pulmonary tissue after acute exposure to industrial cigarette smoke
Germano Duarte Porto
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorDaniela Pacheco dos Santos Haupenthal
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorPriscila Soares Souza
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorGustavo de Bem Silveira
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorRenata Tiscoski Nesi
Biochemistry in Health, Graduate Program in Health Sciences, Medicine School, Pontifícia Universidade Católica do Paraná, Puerto Rico, Brazil
Search for more papers by this authorPaulo Emilio Feuser
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorJonathann Corrêa Possato
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorVanessa Moraes de Andrade
Translational Biomedicine Laboratory, Graduate Program of Health Sciences, Department of Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorRicardo Aurino Pinho
Biochemistry in Health, Graduate Program in Health Sciences, Medicine School, Pontifícia Universidade Católica do Paraná, Puerto Rico, Brazil
Search for more papers by this authorCorresponding Author
Paulo Cesar Lock Silveira
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Correspondence
Paulo Cesar Lock Silveira, UNESC – Universidade do Extremo Sul Catarinense PPGCS – Programa de Pós-Graduação em Ciências da Saúde, Av. Universitária, 1105 – Bairro Universitário, Criciúma – SC CEP, 88806-000, Brazil.
Email: [email protected]
Search for more papers by this authorGermano Duarte Porto
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorDaniela Pacheco dos Santos Haupenthal
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorPriscila Soares Souza
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorGustavo de Bem Silveira
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorRenata Tiscoski Nesi
Biochemistry in Health, Graduate Program in Health Sciences, Medicine School, Pontifícia Universidade Católica do Paraná, Puerto Rico, Brazil
Search for more papers by this authorPaulo Emilio Feuser
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorJonathann Corrêa Possato
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorVanessa Moraes de Andrade
Translational Biomedicine Laboratory, Graduate Program of Health Sciences, Department of Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Search for more papers by this authorRicardo Aurino Pinho
Biochemistry in Health, Graduate Program in Health Sciences, Medicine School, Pontifícia Universidade Católica do Paraná, Puerto Rico, Brazil
Search for more papers by this authorCorresponding Author
Paulo Cesar Lock Silveira
Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil
Correspondence
Paulo Cesar Lock Silveira, UNESC – Universidade do Extremo Sul Catarinense PPGCS – Programa de Pós-Graduação em Ciências da Saúde, Av. Universitária, 1105 – Bairro Universitário, Criciúma – SC CEP, 88806-000, Brazil.
Email: [email protected]
Search for more papers by this authorFunding information: This work was supported by CAPES-001, FAPESC, CNPQ, and UNESC.
Abstract
Inhalation of harmful particles appears as a primary factor for the onset and establishment of chronic obstructive pulmonary disease (COPD). Cigarette smoke acutely promotes an exacerbated inflammatory response with oxidative stress induction with DNA damage. Administration of Gold Nanoparticles (GNPs) with 20 nm in different concentrations can revert damages caused by external aggravations. The effects of GNPs in a COPD process have not been observed until now. The objective of this work was to evaluate the therapeutic effects of intranasal administration of different doses of GNPs after acute exposure to industrial cigarette smoke. Thirty male Swiss mice were randomly divided into five groups: Sham; cigarette smoke (CS); CS + GNPs 2.5 mg/L; CS + GNPs 7.5 mg/L and CS + GNPs 22.5 mg/L. The animals were exposed to the commercial cigarette with filter in an acrylic inhalation chamber and treated with intranasal GNPs for five consecutive days. The results demonstrate that exposure to CS causes an increase in inflammatory cytokines, histological changes, oxidative and nitrosive damage in the lung, as well as increased damage to the DNA of liver cells, blood plasma and lung. Among the three doses of GNPs (2.5, 7.5, and 22.5 mg/L) used, the highest dose had better anti-inflammatory effects. However, GNPs at a dose of 7.5 mg/L showed better efficacies in reducing ROS formation, alveolar diameter, and the number of inflammatory cells in histology, in addition to significantly reduced rate of DNA damage in lung cells without additional systemic genotoxicity already caused by cigarette smoke.
CONFLICT OF INTEREST
The authors declare no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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