In vitro investigation of antioxidant, anti-Inflammatory, and antiplatelet adhesion properties of genistein-modified poly(ethersulfone)/poly(vinylpyrrolidone) hemodialysis membranes
Teng Chang
Department of Polymer Engineering, University of Akron, Akron, Ohio, 44325
Search for more papers by this authorLinda DeFine
Department of Pathology and Laboratory Medicine, Summa Health System, Akron, Ohio, 44309
Search for more papers by this authorThomas Alexander
Department of Pathology and Laboratory Medicine, Summa Health System, Akron, Ohio, 44309
Search for more papers by this authorCorresponding Author
Thein Kyu
Department of Polymer Engineering, University of Akron, Akron, Ohio, 44325
Correspondence to: T. Kyu ([email protected])Search for more papers by this authorTeng Chang
Department of Polymer Engineering, University of Akron, Akron, Ohio, 44325
Search for more papers by this authorLinda DeFine
Department of Pathology and Laboratory Medicine, Summa Health System, Akron, Ohio, 44309
Search for more papers by this authorThomas Alexander
Department of Pathology and Laboratory Medicine, Summa Health System, Akron, Ohio, 44309
Search for more papers by this authorCorresponding Author
Thein Kyu
Department of Polymer Engineering, University of Akron, Akron, Ohio, 44325
Correspondence to: T. Kyu ([email protected])Search for more papers by this authorAbstract
Hemocompatibility of genistein-modified poly(ethersulfone)/poly(vinylpyrrolidone) (PES/PVP) hemodialysis (HD) membranes has been investigated in vitro with emphasis on evaluation of cell viability, antioxidant, anti-inflammatory, and antiplatelet adhesion properties. Genistein modified PES/PVP membranes reveal significant reduction of the reactive oxygen species and also considerable suppression of interleukin-1β and tumor necrosis factor-α levels in whole blood, but to a lesser extent ininterleukin-6. The incorporation of PVP into the HD membrane reduces platelet adhesion by virtue of its hydrophilicity. Of particular importance is that platelet adhesion of the genistein modified membranes declines noticeably at low concentrations of genistein for about 5–10%, beyond which it raises the number of adhered platelets. The initial decline in the platelet adhesion is attributable to genistein's ability to inhibit intercellular and/or vascular cell adhesion, whereas the reversal of this adhesion trend with further increase of genistein loading is ascribed to the inherent hydrophobicity of the genistein modified HD membrane. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 539–547, 2015.
REFERENCES
- 1 Descamps-Latscha B, Drüeke T, Witko-Sarsat V. Dialysis-induced oxidative stress: Biological aspects, clinical consequences, and therapy. Semin Dial 2001; 14: 193–199.
- 2 Locatelli, F, Canaud B, Eckardt K-U, Stenvinkel P, Wanner C, Zoccali C. Oxidative stress in end-stage renal disease: An emerging threat to patient outcome. Nephrol Dial Transplant 2003; 18: 1272–1280.
- 3 Carracedo J, Ramírez R, Madueño J, Soriano S, Rodríguez-Benot A, Rodríguez M, Martín-Malo A, Aljama P. Cell apoptosis and hemodialysis-induced inflammation. Kidney Int 2002; 61: 89–93.
- 4 Nguyen-Khoa T, Massy ZA, Bandt JPD, Kebede M, Salama L, Lambrey G, Witko-Sarsat V, Drüeke TB, Lacour B, Thévenin M. Oxidative stress and haemodialysis: Role of inflammation and duration of dialysis treatment. Nephrol Dial Transplant 2001; 16: 335–340.
- 5 Amore A, Coppo R. Immunological basis of inflammation in dialysis. Nephrol Dial Transplant 2002; 17: 16–24.
- 6 Yang M-C, Lin W-C. Protein adsorption and platelet adhesion of polysulfone membrane immobilized with chitosan and heparin conjugate. Polym Adv Technol 2003; 14: 103–113.
- 7 Liu T-Y, Lin W-C, Huang L-Y, Chen S-Y, Yang M-C. Hemocompatibility and anaphylatoxin formation of protein-immobilizing polyacrylonitrile hemodialysis membrane. Biomaterials 2005; 26: 1437–1444.
- 8 Morimoto H, Nakao K, Fukuoka K, Sarai A, Yano A, Kihara T, Fukuda S, Wada J, Makino H. Long-term use of vitamin E-coated polysulfone membrane reduces oxidative stress markers in haemodialysis patients. Nephrol Dial Transplant 2005; 20: 2775–2782.
- 9 Polkowski K, Mazurek A. Biological properties of genistein. A review of in vitro and in vivo data. Acta Pol Pharm 2000; 57: 135–155.
- 10 Xu J-W, Ikeda K, Yamori Y. Genistein inhibits expressions of NADPH oxidase p22phox and angiotensin II type 1 receptor in aortic endothelial cells from stroke-prone spontaneously hypertensive rats. Hypertens Res 2004; 27: 675–683.
- 11 Wei H, Bowen R, Cai Q, Barnes S, Wang Y. Antioxidant and antipromotional effects of the soybean isoflavone genistein. Exp Biol Med 1995; 208: 124–130.
- 12 Verdrengh M, Jonsson IM, Holmdahl R, Tarkowski A. Genistein as an anti-inflammatory agent. Inflamm Res 2003; 52: 341–346.
- 13 Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 2004; 96: 229–245.
- 14 Rimbach G, Weinberg PD, de Pascual-Teresa S, Alonso MG, Ewins BA, Turner R, Minihane AM, Botting N, Fairley B, Matsugo S, Uchida Y, Cassidy A. Sulfation of genistein alters its antioxidant properties and its effect on platelet aggregation and monocyte and endothelial function. Biochim Biophys Acta 2004; 1670: 229–237.
- 15 Neelakandan C, Chang T, Alexander T, DeFine L, Evancho-Chapman M, Kyu T. In vitro evaluation of antioxidant and anti-inflammatory properties of genistein-modified hemodialysis membranes. Biomacromolecules 2011; 12: 2447–2455.
- 16 Barth C, Gonçalves MC, Pires ATN, Roeder J, Wolf BA. Asymmetric polysulfone and polyethersulfone membranes: Effects of thermodynamic conditions during formation on their performance. J Membr Sci 2000; 169: 287–299.
- 17
Locatelli F,
Ronco C,
Tetta C. Polyethersulfone: Membranes for Multiple Clinical Applications. Basel: Karger, S. Inc; 2002.
10.1159/isbn.978-3-318-00903-3 Google Scholar
- 18 Wavhal DS, Fisher ER. Hydrophilic modification of polyethersulfone membranes by low temperature plasma-induced graft polymerization. J Memb Sci 2002; 209: 255–269.
- 19 Neelakandan C, Kyu T. Miscibility characterization in relation to phase morphology of poly(ether sulfone)/poly(vinyl pyrrolidone) blends containing a phytochemical. J Phys Chem B 2009; 113: 8520–8526.
- 20 Marchese J, Ponce M, Ochoa NA, Prádanos P, Palacio L, Hernández A. Fouling behaviour of polyethersulfone UF membranes made with different PVP. J Memb Sci 2003; 211: 1–11.
- 21 Okano T, Nishiyama S, Shinohara I, Akaike T, Sakurai Y, Kataoka K, Tsuruta T. Effect of hydrophilic and hydrophobic microdomains on mode of interaction between block polymer and blood platelets. J Biomed Mater Res 1981; 15: 393–402.
- 22 Tamada Y, Kulik E, Ikada Y. Simple method for platelet counting. Biomaterials 1995; 16: 259–261.
- 23 Watanabe W, Sudo K, Asawa S, Konno K, Yokota T, Shigeta S. Use of lactate dehydrogenase to evaluate the anti-viral activity against influenza A virus. J Virol Methods 1995; 51: 185–191.
- 24 Chenoweth DE, Cheung K, Henderson LW. Anaphylatoxin formation during hemodialysis: Effects of different dialyzer membranes. Kidney Int 1983; 24: 764–769.
- 25 Shepro D, Belamarich FA, Merk FB, Chao FC. Changes in thrombocyte ultrastructure during clot retraction. J Cell Sci 1969; 4: 763–779.
- 26 Kruk I, Aboul-Enein HY, Michalska T, Lichszteld K, Kladna A. Scavenging of reactive oxygen species by the plant phenols genistein and oleuropein. Luminescence 2005; 20: 81–89.
- 27 Zhang H-Y, Wang L-F, Sun Y-M. Why B-ring is the active center for genistein to scavenge peroxyl radical: A DFT study. Bioorg Med Chem Lett 2003; 13: 909–911.
- 28 Li J, Shah A M. ROS generation by nonphagocytic NADPH oxidase: Potential relevance in diabetic nephropathy. J Am Soc Nephrol 2003; 14: 221–226.
- 29 Apel K, Hirt H. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 2004; 55: 373–399.
- 30 Geng Y, Zhang B, Lotz M. Protein tyrosine kinase activation is required for lipopolysaccharide induction of cytokines in human blood monocytes. J Immunol 1993; 151: 6692–6700.
- 31 Davis J, Kucuk O, Djuric Z, Sarkar F. Soy isoflavone supplementation in healthy men prevents NF-κB activation by TNF-α in blood lymphocytes. Free Radical Biomed 2001; 30: 1293–1302.
- 32 Schindler R, Lonnemann G, Shaldon S, Koch KM, Dinarello C. Transcription, not synthesis, of interleukin-1 and tumor necrosis factor by complement. Kidney Int 1990; 37: 85–93.
- 33 Lafreniere LY, Talbot FDF, Matsuura T, Sourirajan S. Effect of polyvinylpyrrolidone additive on the performance of polyethersulfone ultrafiltration membranes. Ind Eng Chem Res 1987; 26: 2385–2389.
- 34 Lee JH, Ju YM, Kim DM. Platelet adhesion onto segmented polyurethane film surfaces modified by addition and crosslinking of PEO-containing block copolymers. Biomaterials 2000; 21: 683–691.
- 35 Gölander C-G, Jönsson S, Vladkova T, Stenius P, Eriksson J C. Preparation and protein adsorption properties of photopolymerized hydrophilic films containing N-vinylpyrrolidone (NVP), acrylic acid (AA) or ethyleneoxide (EO) units as studied by ESCA. Colloid Surf 1986; 21: 149–165.
- 36 Evangelista V, Manarini S, Sideri R, Rotondo S, Martelli N, Piccoli A, Totani L, Piccardoni P, Vestweber D, de Gaetano G, CerlettiC. Platelet/polymorphonuclear leukocyte interaction: P-selectin triggers protein–tyrosine phosphorylation-dependent CD11b/CD18 adhesion: Role of PSGL-1 as a signaling molecule. Blood 1999; 93: 876–885.
- 37 Tsunoda N, Kokubo K, Sakai K, Fukuda M, Miyazaki M, Hiyoshi T. Surface roughness of cellulose hollow fiber dialysis membranes and platelet adhesion. ASAIO J 1999; 45: 418–423.
- 38 Zingg W, Neumann AW, Strong AB, Hum OS, Absolom DR. Effect of surface roughness on platelet adhesion under static and under flow conditions. Can J Surg 1982; 25: 16–19.
